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Method for extracting ginsenoside by biomimetic method and ginsenoside extract by using method

A ginsenoside and biomimetic extraction technology is applied in pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., which can solve the problems of low extraction efficiency, long extraction time, and inability to invest, and achieve high extraction efficiency and solvent consumption. Small volume, good accuracy

Inactive Publication Date: 2020-07-28
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the extraction methods have deficiencies, such as: long extraction time, low extraction efficiency, large amount of solvent, etc., so that the above methods cannot be put into industrial production at present, and the extraction of ginsenosides still needs to be solved for further research on its activity. main problem

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  • Method for extracting ginsenoside by biomimetic method and ginsenoside extract by using method
  • Method for extracting ginsenoside by biomimetic method and ginsenoside extract by using method
  • Method for extracting ginsenoside by biomimetic method and ginsenoside extract by using method

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Embodiment 1

[0041] The present embodiment provides a process for extracting ginsenosides using pepsin biomimetic method, which is mainly carried out as follows:

[0042] Fresh ginseng is washed, dried and crushed to obtain ginseng powder. Select ginseng that has been planted for more than 4 years, wash it with water until there is no sludge on the surface, dry the ginseng in a blast drying oven at 45°C to constant weight, crush the dried ginseng with a pulverizer, and pass it through a 60-mesh sieve. Add 1.64mL, 2.34mol / L dilute hydrochloric acid solution to 80mL deionized water, weigh 1.000g pepsin powder and dissolve in it, and dilute to 100mL with deionized water to obtain 10mg / mL bionic gastric juice. Among them, the specific activity of pepsin is not lower than 1:3000. Take 1g of ginseng powder and 15mL of bionic gastric juice, heat and reflux at 37°C under normal pressure, extract for 60 minutes, and centrifuge at 8000rpm for 8 minutes to separate the extract and ginseng powder. T...

Embodiment 2

[0046] The present embodiment provides a technique for extracting ginsenosides by biomimetic method using trypsin, which is mainly carried out as follows:

[0047] Fresh ginseng is washed, dried and crushed to obtain ginseng powder. Select ginseng that has been planted for more than 4 years, wash it with water until there is no sludge on the surface, dry the ginseng in a blast drying oven at 45°C to constant weight, crush the dried ginseng with a pulverizer, and pass it through a 60-mesh sieve. Weigh 0.680 g of potassium dihydrogen phosphate, ultrasonically dissolve it in 50 mL of deionized water, and adjust the pH value to 6.8 with 0.1 mol / L sodium hydroxide aqueous solution. Weigh 1.000 g of trypsin and add it to the prepared phosphate buffer solution, dissolve it by ultrasonic and dilute it to 100 mL with deionized water to obtain 10 mg / mL bionic intestinal juice. Take 1g of ginseng powder and 15mL of bionic intestinal juice, heat and reflux at 37°C under normal pressure, ...

Embodiment 3

[0051] This embodiment provides a preparation method of artificial gastric juice applied to the bionic extraction of ginsenosides:

[0052] Add 16.4mL, 2.34mol / L dilute hydrochloric acid solution to 800mL deionized water, weigh 10.00g pepsin powder and dissolve in it, and dilute to 1000mL with deionized water to obtain 10mg / mL bionic gastric juice, and its UV absorption spectrum See Figure 4 .

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Abstract

The invention discloses a method for extracting ginsenoside by a biomimetic method and a ginsenoside extract by using the method. The extraction method comprises the steps of preparation of a biomimetic extraction solvent, biomimetic extraction of ginsenoside and extraction of an extracting liquid, wherein the biomimetic extraction solvent is biotic gastric juice or biotic intestinal juice; in thestep of biomimetic extraction of ginsenoside, ginseng is extracted by utilizing the prepared biomimetic extraction solvent after being dried and ground, the extracting liquid is centrifuged and filtered, and the extraction solvent is utilized to extract; in the step of extraction of the extracting liquid, the extracting liquid and the extraction solvent are mixed and separated, and the ginsenoside extract is obtained after the extracting liquid volatized and dried. by utilizing the biomimetic extraction method, the ginsenoside extract has high content of effective ingredients. Compared with atraditional organic solvent extraction method, the method has the characteristics of environmental friendliness, no toxicity or harm and high extraction efficiency. The method is low in energy consumption, short in time consumption, small in solvent consumption and simple and convenient to operate, has good accuracy, repeatability and precision, and is applicable to industrial production.

Description

technical field [0001] The invention relates to the technical field of extraction of natural extracts, in particular to a method for extracting ginsenosides from ginseng by using a bionic technology and the extracted ginsenoside extracts. Background technique [0002] Ginseng (Panax ginseng C.A.Mayer) is a perennial herb of the Araliaceae genus Panax, mainly produced in East Asia. It is a traditional precious Chinese medicine in my country and has a medicinal basis for thousands of years. Ginseng is suitable for regulating blood pressure, restoring heart function, treating neurasthenia and physical weakness, and also has anti-allergic, anti-tumor, and hypoglycemic effects. Ginseng contains ginsenosides, polysaccharides, volatile oils, proteins, amino acids and other ingredients, among which ginsenosides are the most important active ingredients. [0003] Ginsenoside is a kind of sterol compound, triterpene saponin, which mainly exists in Panax genus medicinal materials. Ac...

Claims

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Application Information

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IPC IPC(8): A61K36/258
CPCA61K36/258A61K2236/19A61K2236/333A61K2236/55
Inventor 修洋赵幻希万茜淋王秋颖孙秀丽
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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