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Method for analyzing drug resistance of tumor cells

A technology of tumor cells and drug resistance, applied in the field of tumor treatment, can solve problems such as failure of tumor chemotherapy, and achieve simple and efficient results

Pending Publication Date: 2020-07-24
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic and foreign studies have shown that tumor multidrug resistance is the main reason for the failure of tumor chemotherapy

Method used

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  • Method for analyzing drug resistance of tumor cells
  • Method for analyzing drug resistance of tumor cells

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Dissolve 0.4g aminodextran in 25mL water and heat until dissolved. The temperature was then lowered to 30°C. Nitrogen gas was passed for 1 h, and dilute nitric acid solution containing 0.05 g of ceric ammonium nitrate was added under the protection of nitrogen gas. After 5 min, 0.06 mL of methyl acrylate was added. After 0.5h, add 0.012g of diallyl disulfide and react for 4h. After the reaction was completed, it was dialyzed at room temperature for 3 days to obtain aminodextran nanoparticles.

[0039]The aminodextran nanoparticles were dissolved in water, and the pH was adjusted to 8.5 with alkali to form an aqueous solution of aminodextran nanoparticles with a concentration of 8 mg / mL. The cyanine dye CY5.5 succinimide was dissolved in dimethyl sulfoxide to form a cyanine dye CY5.5 succinimide dimethyl sulfoxide solution (CY5.5-NHS solution) at a concentration of 10 mg / mL. The aminodextran nanoparticle solution and the CY5.5-NHS solution are mixed according to a ma...

Embodiment 2

[0041] Dissolve 1g of hydroxymethyl chitosan in 25mL of water and heat until dissolved. The temperature was lowered to 30°C. Nitrogen was passed for 1 hour, and dilute nitric acid solution containing 0.06 g of ceric ammonium nitrate was added under the protection of nitrogen. After 5 min, 0.05 mL of methyl acrylate was added. After 0.5h, add 0.012g of diallyl disulfide and react for 4h. After the reaction was completed, it was dialyzed for 3 days to obtain hydroxymethyl chitosan nanoparticles.

[0042] The hydroxymethyl chitosan nanoparticles were dissolved in water to form an aqueous solution of hydroxymethyl chitosan nanoparticles with a concentration of 5 mg / mL. The cyanine dye CY5.5 succinimide was dissolved in dimethyl sulfoxide to form a cyanine dye CY5.5 succinimide dimethyl sulfoxide solution (CY5.5-NHS solution) at a concentration of 10 mg / mL. The hydroxymethyl chitosan nano particle solution and the CY5.5-NHS solution are mixed according to a mixing ratio of 50-2...

Embodiment 3

[0044] Dissolve 0.5 g of dextran (molecular weight of 40,000 Da) and 0.5 g of chitosan in 25 mL of water and heat until dissolved. The temperature was lowered to 30°C. Nitrogen was blown for 1 hour, and dilute nitric acid solution containing 0.06 g of cerium ammonium nitrate was added under the protection of nitrogen, 0.05 mL of methyl acrylate was added after 5 minutes, 0.012 g of diallyl disulfide was added after 0.5 hours, and the reaction was carried out for 4 hours. After the reaction was completed, it was dialyzed for 3 days to obtain dextran / chitosan nanoparticles.

[0045] Dextran / chitosan nanoparticles were dissolved in water to form an aqueous solution of dextran / chitosan nanoparticles with a concentration of 10 mg / mL. Dissolve the graphene quantum dots in water to form an aqueous solution of graphene quantum dots with a concentration of 2 mg / mL. The dextran / chitosan nano particle aqueous solution and the graphene quantum dot aqueous solution are mixed according to...

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Abstract

The invention discloses a method for analyzing drug resistance of tumor cells. The method comprises the following steps: (a) providing polysaccharide-based nanoparticles; (b) adding the polysaccharide-based nanoparticles into a tumor cell culture medium; and (c) detecting the affinity of the tumor cells and the polysaccharide-based nanoparticles marked by functional molecules. According to the analysis method, the drug-resistant tumor cells can be clearly, visually and efficiently analyzed; after the polysaccharide-based nanoparticles and tumor cells are jointly hatched, a flow cytometer or alaser confocal microscope is adopted for detection, the drug resistance of the tumor cells and the proportion of drug-resistant cells are judged according to a detection result, and the method is simple and efficient.

Description

technical field [0001] The invention relates to the field of tumor treatment, in particular to a method for analyzing drug resistance of tumor cells. Background technique [0002] Tumors seriously endanger human health. In recent years, the morbidity and mortality of tumors have been increasing year by year. At present, clinical tumor treatment mainly includes surgical resection, chemical drug therapy, radiotherapy, gene therapy and cell therapy, among which the use of chemical drug therapy is the most common. But the problem it faces is that when using chemical drugs, it is easy to make tumor cells develop drug resistance, which leads to the failure of tumor chemotherapy. We call this drug resistance induced by a chemotherapeutic drug during chemotherapy as acquired drug resistance. In addition, some tumor cells already have drug resistance before starting chemotherapy, which is called natural drug resistance. Acquired drug resistance can be further divided into original...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCG01N33/5011
Inventor 窦红静王成龙许国雄
Owner SHANGHAI JIAO TONG UNIV
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