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Hydrogel particle and preparation method thereof

A hydrogel and microparticle technology is applied in the field of preparing the hydrogel microparticles, carboxymethyl-β-cyclodextrin grafted carboxymethyl chitosan hydrogel microparticles, and can solve allergies, hypoglycemia, infections, etc. problem, to achieve the effect of prolonged release, good biocompatibility, and avoidance of damage and degradation

Pending Publication Date: 2020-07-24
SHANTOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, long-term injections are likely to cause side effects such as allergies, hypoglycemia, infection, and subcutaneous fat atrophy, which brings a lot of trouble and inconvenience to diabetic patients who need life-long medication

Method used

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  • Hydrogel particle and preparation method thereof
  • Hydrogel particle and preparation method thereof
  • Hydrogel particle and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1, preparing carboxymethyl-β-cyclodextrin grafted carboxymethyl chitosan hydrogel particles.

[0032] process such as figure 1 As shown, it mainly includes the following steps:

[0033] (1) Preparation of carboxymethyl-β-cyclodextrin solution: Weigh 4.0g carboxymethyl-β-cyclodextrin and dissolve it in 80mL deionized water to obtain carboxymethyl-β-cyclodextrin solution;

[0034] (2) Prepare carboxymethyl chitosan solution: take 4.0g carboxymethyl chitosan and dissolve in 100 mL deionized water to obtain carboxymethyl chitosan solution;

[0035] (3) Preparation of carboxymethyl-β-cyclodextrin grafted carboxymethyl chitosan hydrogel particles: mix carboxymethyl chitosan solution and carboxymethyl-β-cyclodextrin solution, add 3.68g 1-Ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and 2.21g of N-N-hydroxysuccinimide were grafted and cross-linked, and fully stirred at 25°C for 24 hours to obtain Grafted product solution; the grafted product solution was ...

Embodiment 2

[0036] Example 2, Structural Characterization Determination.

[0037] Fourier transform infrared spectrum detection: Carboxymethyl-β-cyclodextrin, carboxymethyl chitosan and a small amount of KBr are mixed and ground respectively, and pressed into thin slices. The freeze-dried gel sample was analyzed by Fourier transform infrared spectroscopy at a wavelength of 500-4000 cm with the aid of the ATR accessory. -1 range to scan.

[0038] The characterization of carboxymethyl-β-cyclodextrin grafted carboxymethyl chitosan hydrogel particles is shown in the figure below, where, as figure 2 As shown, the three are at 3400cm -1 The peaks at all positions are attributed to the OH group vibration. Carboxymethyl-β-cyclodextrin at 2930, 1601, 1420cm -1 The absorption peaks at are assigned to carboxymethyl aliphatic CH 2 The asymmetric stretching vibration of the group, the asymmetric stretching vibration and the symmetric stretching vibration of the carboxylate group C=O. In the spe...

Embodiment 3

[0045] Embodiment 3, physical and chemical performance measurement index evaluation.

[0046] Swelling experiment: take a certain mass (W 0 ) was placed in 5 mL of 0.1M HCl solution with pH = 1.2 and 0.1% (w / v) PBS phosphate solution with pH = 6.8 and pH = 7.4, and shaken at 37° C. for 2 h at 100 rpm. At 30, 60, 90, and 120 min, gently absorb the surface of excess liquid with filter paper, and weigh (W t ). The swelling rate is calculated with the following formula, and the change curve of the swelling rate with time is drawn.

[0047] Swelling rate: SR=(W t -W 0 ) / W 0 ×100%

[0048] The results clarified the physicochemical properties of carboxymethyl-β-cyclodextrin grafted carboxymethyl chitosan hydrogel particles, in which, Figure 6 It shows that the swelling rate of hydrogel particles is high under different pH conditions, and has excellent swelling performance. Hydrogel particles can not only absorb a large amount of water molecules, but also facilitate the stora...

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Abstract

The invention discloses a hydrogel particle and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a carboxymethyl-beta-cyclodextrin solution; (2) preparing a carboxymethyl chitosan solution; (3) preparing the carboxymethyl-beta-cyclodextrin grafted carboxymethyl chitosan hydrogel particle. The hydrogel particle is obtained by performing grafting andcrosslinking on carboxymethyl-beta-cyclodextrin and carboxymethyl chitosan, the carboxymethyl-beta-cyclodextrin and carboxymethyl chitosan serving as raw materials which are easily available are mainly used, so that use of high-cost raw materials is avoided. and preparation steps are concise, and the preparation process is safe and easy to operate; the hydrogel particle almost has no cytotoxicity,has excellent biocompatibility, can be taken in an orally intragastric manner and does not have toxic or side effect; and the sustained release effect is excellent after insulin is loaded, destruction and degradation of gastric acid and pepsin on insulin can be avoided, drug release can be controlled, the medicinal curative effect can be prolonged, the medicinal activity is kept constant, and thebioavailability of orally-taking insulin protein medicines can be improved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a carboxymethyl-β-cyclodextrin grafted carboxymethyl chitosan hydrogel particle and a method for preparing the hydrogel particle. Background technique [0002] Diabetes is one of the most common chronic diseases in modern people's life and it is difficult to cure. Insulin has always been the main treatment. However, long-term injections are likely to cause side effects such as allergies, hypoglycemia, infection, and subcutaneous fat atrophy, which brings a lot of trouble and inconvenience to diabetic patients who need life-long medication. Oral administration is convenient and reasonable, and is easy to be accepted by patients, so oral insulin is undoubtedly the ideal and best treatment. At present, the oral administration of insulin-like protein needs to overcome the problems of physiological barriers, enzyme barriers and physical and chemical stability. Avoid the destruct...

Claims

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Application Information

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IPC IPC(8): A61K38/28A61K9/52A61K47/40A61K47/36A61P3/10
CPCA61K38/28A61K9/5036A61P3/10
Inventor 刘杨杨宇陈胜勤
Owner SHANTOU UNIV
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