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Biocontrol fungus identification method of soft rot diseases of boilen taros

A technology of biocontrol fungus and identification method, which is applied in the field of biocontrol fungus identification of betel nut taro soft rot disease

Inactive Publication Date: 2020-07-10
NINGDE NORMAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a biocontrol fungal identification method for betel nut taro soft rot disease, which solves the problem of biocontrol fungal identification for preventing and treating betel nut taro soft rot disease

Method used

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  • Biocontrol fungus identification method of soft rot diseases of boilen taros
  • Biocontrol fungus identification method of soft rot diseases of boilen taros
  • Biocontrol fungus identification method of soft rot diseases of boilen taros

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Embodiment

[0046] The embodiment of the present invention provides the biocontrol fungus identification method of betel nut taro soft rot disease, comprising the following steps:

[0047] S1. Test material preparation

[0048] Materials: Soil samples and healthy betel nut taro bulbs were collected from the betel nut taro planting fields in Hekeng Village, Guanling Town, Fuding City, Fujian Province. Different planting plots were selected, and the soil samples were taken from the rhizosphere soil of healthy betel nut taro plants at a depth of 10-15cm 200g each in 3 places, 5 healthy betel nut taro bulbs, put them in an incubator equipped with ice packs after marking, and take them back for later use. The pathogenic bacteria of soft rot of betel nut taro came from the strains isolated and identified in the laboratory.

[0049] S2. Isolation and purification of fungi

[0050] Isolation of fungi from soil: Weigh 10g of soil into a glass pearl triangle bottle filled with 90mL of sterile wat...

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Abstract

The invention provides a biocontrol fungus identification method of soft rot diseases of boilen taros, and relates to the technical field of biological control. The biocontrol fungus identification method of soft rot diseases of boilen taros comprises the steps of preparing experiment materials, performing separation and purification on fungi, performing in vitro stem tuber returning and screeningtest, observing the shape of a bacterial strain, performing strain molecular identification, screening the growth characteristics of the bacterial strain, and performing field living body control effect test on the strain plants. 22 fungi obtained by separation and purification from Fuding boilen taro rhizosphere soil and healthy plant corms are identified, and in vitro returning and field livingbody control effect test is used for identifying the biocontrol effect of boilen taro bacterial strains. A molecule sequencing method is adopted for screening independent unduplicated bacterial strains for performing conventional shape characteristic and physiological and biochemical test measurement, and the best screening method is researched, so that a research reference is provided for biological control of the soft rot diseases of the boilen taros in the future, and the method is worth of being widely popularized.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a method for identifying fungi for biological control of taro soft rot disease. Background technique [0002] Betel nut taro is a plant of the genus Araceae. Its plant height is about 1.3m. Each plant has a mother taro and a small taro, which grow vigorously. Betel nut taro has a strong aroma and soft taste after cooking, so it is also called taro. Betel nut taro has high nutritional value, rich in protein, starch, minerals and multivitamins, and has the effects of nourishing the spleen and stomach, nourishing qi and nourishing the kidney. , because it is an excellent raw material for daily cooking and has a nourishing effect, the market demand is broad. [0003] Soft rot is the most important disease of betel nut taro. In recent years, the damage caused by agricultural production has become increasingly serious. The incidence rate of plants is generally 3% to 15%. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/04C12Q1/18C12R1/645
CPCC12Q1/04C12Q1/18C12Q1/6895G01N2333/37
Inventor 董晓菲叶祖云周银珠陈美霞刘盛荣
Owner NINGDE NORMAL UNIV
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