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A sex-specific molecular marker primer for pompano ovata and its application

A specific technology for ovoid pomfret, applied in biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc. Anatomy of individual death and other issues to achieve the effect of avoiding inbreeding depression, breaking through technical bottlenecks, and high accuracy

Active Publication Date: 2020-12-04
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) In scientific research work, the biological sex of pomfret can be identified through dissection and other methods, but dissection will inevitably lead to the death of the dissected individual, and this method is inefficient and cannot achieve large-scale identification
[0005] (2) In production practice, it is impossible to identify the sex of live pomfrets by phenotype
[0006] (3) There is currently no technology for live sex identification through biotechnological means

Method used

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  • A sex-specific molecular marker primer for pompano ovata and its application

Examples

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Embodiment 1

[0030] The sex-specific molecular marker primers for ovoid pomfret provided in this example, the primers are primers P2, and the primers P2 include forward primers P2-F and reverse primers P2-R, and the forward primers P2-F The base sequence of the reverse primer P2-R is shown in SEQ ID NO:1, and the base sequence of the reverse primer P2-R is shown in SEQ ID NO:2.

[0031] Specifically, the base sequence of primer P2 is as follows:

[0032] Forward primer P2-F: 5'-CATGGACAAGAAGGTGGTGC-3';

[0033] Reverse primer P2-R: 5'-TACCCAGTGCAAGCTCTCTC-3'.

[0034] A full-sib family of Pompano ovata was collected, including 2 parents and 100 offspring individuals, and the physiological sex was identified after dissection. High-throughput sequencing was used to mark the parents and offspring with the genome sequence of the pomfret pomfret as a reference sequence, and linkage analysis was used to locate the sex quantitative trait loci and locate them in a single interval. Development o...

Embodiment 2

[0040] The method for male and female identification of oval pomfret provided by this embodiment comprises the following steps: using PCR amplification to amplify specific fragments in the genomic DNA of male and female individuals, specifically including: designing the primers in Example 1, according to the embodiment The method in 1 extracts the genomic DNA of the ovoid pomfret to be tested, adopts the primers in Example 1, and uses the extracted genomic DNA as a template to carry out PCR amplification, using gel electrophoresis (specifically, an automatic sequencer ABI 3730XL can be used) The amplified products were detected, and male and female individuals were identified according to the electrophoresis results.

[0041] The object of implementation in this embodiment is pomfret ovata, and 47 females and 47 males were taken, and the sexes were identified through anatomy, and then the feasibility of the primer was verified.

[0042] (1) Extraction of DNA

[0043] Cut abou...

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Abstract

The invention discloses a trachinotus ovatus gender specific molecular marker primer. The primer is a primer P2 which comprises a forward primer P2-F and a reverse primer P2-R; and the base sequencesof the forward primer P2-F and the reverse primer P2-R are respectively as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The invention also discloses a test kit used for gender determination of trachinotusovatus, and an application of the above-mentioned primer or the test kit in gender determination of the trachinotus ovatus. The primer provided by the invention is good in specificity, high in sensitivity and high in accuracy, does not harm trachinotus ovatus living bodies, can quickly and accurately identify the gender of the trachinotus ovatus in batches at a time, and has important scientific research value and practical application value.

Description

technical field [0001] The invention belongs to the technical field of sex of ovoid pomfret, and in particular relates to a sex-specific molecular marker primer and application thereof. Background technique [0002] Oval pomfret (Trachinotus ovatus), belongs to Perciformes, Trevalidae, Pomfret genus, commonly known as golden pomfret, yellow pomfret, yellow waxed pomfret. After the breakthrough of artificial induced spawning in 2000, large-scale aquaculture began in my country. At present, it has developed into an important marine aquaculture species in China, with an output of more than 100,000 tons. This species has the characteristics of rapid growth, strong disease resistance, and turbulent flow. The best species for large-scale farming in deep and open seas in the future. Although the scale of its breeding has increased, the breeding work has lagged behind. The important limiting factor is that the oval pomfret can't distinguish the sex from its appearance, which makes i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6879C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6879C12Q1/6888C12Q2565/125
Inventor 张殿昌郭梁杨静文刘宝锁郭华阳朱克诚张楠杨权
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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