Preparation method of composite Pickering emulsion for improving survival rate of probiotics

A technology of Pickering emulsion and composite emulsion, which is applied in the field of preparation of composite Pickering emulsion, can solve problems such as low survival rate, and achieve the effects of stable protection, high content of probiotics, and improved stability

Inactive Publication Date: 2020-07-03
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem of low survival rate of traditional probiotics reaching the intestinal tract during the digestion process, the present invention proposes a novel preparation method for compound Pickering emulsion that improves the survival rate of probiotics

Method used

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  • Preparation method of composite Pickering emulsion for improving survival rate of probiotics
  • Preparation method of composite Pickering emulsion for improving survival rate of probiotics
  • Preparation method of composite Pickering emulsion for improving survival rate of probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of the oil phase: Add Span-80 with a volume ratio of 2% as the primary emulsifier to the evening primrose oil, and stir well to obtain the oil phase of the primary emulsion;

[0024] W 1 The preparation of / O primary emulsion: the probiotic suspension after washing is used as internal phase solution (W 1 ) was dispersed into the oil phase (O) composed of evening primrose oil and lipophilic emulsifier Span-80, the oil phase accounted for 50% by volume, the mixture was magnetically stirred for 5min at 200rpm, and then again at 4000rpm Use a homogenizer to homogenize for 3 minutes, emulsify for 15 seconds, and pause for 10 seconds to prepare the primary emulsion W 1 / O;

[0025] Preparation of β-cyclodextrin aqueous solution: Accurately weigh 2.5g of β-cyclodextrin, add it to 97.5g of deionized water to make the mass concentration of β-cyclodextrin 2.5%, and magnetically stir in a water bath at 70°C for 10 minutes , cooled to room temperature;

[0026] W 1...

Embodiment 2

[0028] Preparation of the oil phase: Add Span-80 with a volume ratio of 4% as the primary emulsifier to the evening primrose oil, and stir evenly to obtain the oil phase of the primary emulsion;

[0029] W 1 The preparation of / O primary emulsion: the probiotic suspension after washing is used as internal phase solution (W 1 ) into the oil phase (O) composed of evening primrose oil and lipophilic emulsifier Span-80, the volume percentage of the oil phase is 60%, the mixture is magnetically stirred for 6min under the condition of 300rpm, and then the Use a homogenizer to homogenize for 4 minutes, emulsify for 15 seconds, and pause for 10 seconds to prepare the primary emulsion W 1 / O;

[0030] Preparation of β-cyclodextrin aqueous solution: Accurately weigh 3g of β-cyclodextrin, add it to 97g of deionized water to make the mass concentration of β-cyclodextrin 3%, and continue the magnetic stirring treatment in a water bath at 70°C for 12min, cool to room temperature;

[003...

Embodiment 3

[0033] Preparation of the oil phase: Add Span-80 with a volume ratio of 6% as the primary emulsifier to the evening primrose oil, and stir well to obtain the oil phase of the primary emulsion;

[0034] W 1 The preparation of / O primary emulsion: the probiotic suspension after washing is used as internal phase solution (W 1 ) into the oil phase (O) composed of evening primrose oil and lipophilic emulsifier Span-80, the oil phase accounted for 70% by volume, the mixture was magnetically stirred for 7min at 300rpm, and then again at 5000rpm Use a homogenizer to homogenize for 4 minutes, emulsify for 15 seconds, and pause for 10 seconds to prepare the primary emulsion W 1 / O;

[0035] Preparation of β-cyclodextrin aqueous solution: Accurately weigh 3.5g of β-cyclodextrin, add it to 96.5g of deionized water, so that the mass concentration of β-cyclodextrin is 3.5%, and magnetically stir in a water bath at 70°C for 13 minutes , cooled to room temperature;

[0036] W 1 / O / W 2 P...

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Abstract

The invention belongs to the technical field of preparation of probiotics, and relates to a preparation method of a composite Pickering emulsion for improving the survival rate of probiotics. The preparation method comprises the following steps: adding span-80 into evening primrose oil, uniformly stirring the span-80 and the evening primrose oil to prepare an oil phase, adding a washed probioticssolution in the oil phase, and carrying out homogenizing to obtain a product A; and mixing a cyclodextrin aqueous solution with the product A to obtain the composite emulsion. According to the preparation method, a double-layer emulsification system is adopted, firstly, a high internal phase processing technology can ensure high content of probiotics in an internal water phase, secondly, the antioxidant activity of the evening primrose oil phase can prevent the probiotics from being oxidized and damaged after the probiotics are stored and orally taken, and in addition, a nano-scale Pickering emulsion wall layer formed by beta-cyclodextrin on the outer layer has a very stable protection effect, can protect the probiotics from being damaged when passing through the stomach and the small intestine, improves the survival rate of the probiotics, and enables the probiotics to smoothly reach the large intestine to play a role. The preparation method can be used for developing novel probioticsfoods, medicines and health products.

Description

technical field [0001] The invention belongs to the technical field of probiotic preparation, and relates to a method for preparing a compound Pickering emulsion for improving the survival rate of probiotics. Background technique [0002] Probiotics refer to active microorganisms that are beneficial to the host. Probiotics can promote the digestion and absorption of nutrients, improve host immunity and maintain intestinal health by regulating the balance of intestinal flora or by regulating the function of the host mucosa and immune system . Common probiotics mainly include yeast, lactobacilli, probiotic spores, and actinomycetes. Among them, Lactobacillus helveticus is a kind of Lactobacillus, which is a Gram-positive bacterium with a long rod shape, no flagella, no spores, milky white colonies, neat edges, and facultative anaerobic. Compared with other probiotics, Lactobacillus helveticus has stronger proteolytic activity, higher polypeptide content in fermented dairy pr...

Claims

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Application Information

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IPC IPC(8): A61K9/113A61K47/44A61K47/40A61K47/26A61K35/741A23L33/135A23L29/10A23L29/00A23L29/30
CPCA23V2002/00A61K9/113A61K35/741A61K47/26A61K47/40A61K47/44A23L29/03A23L29/10A23L29/37A23L33/135A23V2200/222A23V2200/3204A23V2250/5112A23V2300/26
Inventor 袁超崔波田亚超于滨郭丽吴正宗刘鹏飞陶海腾
Owner QILU UNIV OF TECH
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