Preparation method of composite Pickering emulsion for improving survival rate of probiotics
A technology of Pickering emulsion and composite emulsion, which is applied in the field of preparation of composite Pickering emulsion, can solve problems such as low survival rate, and achieve the effects of stable protection, high content of probiotics, and improved stability
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Embodiment 1
[0023] Preparation of the oil phase: Add Span-80 with a volume ratio of 2% as the primary emulsifier to the evening primrose oil, and stir well to obtain the oil phase of the primary emulsion;
[0024] W 1 The preparation of / O primary emulsion: the probiotic suspension after washing is used as internal phase solution (W 1 ) was dispersed into the oil phase (O) composed of evening primrose oil and lipophilic emulsifier Span-80, the oil phase accounted for 50% by volume, the mixture was magnetically stirred for 5min at 200rpm, and then again at 4000rpm Use a homogenizer to homogenize for 3 minutes, emulsify for 15 seconds, and pause for 10 seconds to prepare the primary emulsion W 1 / O;
[0025] Preparation of β-cyclodextrin aqueous solution: Accurately weigh 2.5g of β-cyclodextrin, add it to 97.5g of deionized water to make the mass concentration of β-cyclodextrin 2.5%, and magnetically stir in a water bath at 70°C for 10 minutes , cooled to room temperature;
[0026] W 1...
Embodiment 2
[0028] Preparation of the oil phase: Add Span-80 with a volume ratio of 4% as the primary emulsifier to the evening primrose oil, and stir evenly to obtain the oil phase of the primary emulsion;
[0029] W 1 The preparation of / O primary emulsion: the probiotic suspension after washing is used as internal phase solution (W 1 ) into the oil phase (O) composed of evening primrose oil and lipophilic emulsifier Span-80, the volume percentage of the oil phase is 60%, the mixture is magnetically stirred for 6min under the condition of 300rpm, and then the Use a homogenizer to homogenize for 4 minutes, emulsify for 15 seconds, and pause for 10 seconds to prepare the primary emulsion W 1 / O;
[0030] Preparation of β-cyclodextrin aqueous solution: Accurately weigh 3g of β-cyclodextrin, add it to 97g of deionized water to make the mass concentration of β-cyclodextrin 3%, and continue the magnetic stirring treatment in a water bath at 70°C for 12min, cool to room temperature;
[003...
Embodiment 3
[0033] Preparation of the oil phase: Add Span-80 with a volume ratio of 6% as the primary emulsifier to the evening primrose oil, and stir well to obtain the oil phase of the primary emulsion;
[0034] W 1 The preparation of / O primary emulsion: the probiotic suspension after washing is used as internal phase solution (W 1 ) into the oil phase (O) composed of evening primrose oil and lipophilic emulsifier Span-80, the oil phase accounted for 70% by volume, the mixture was magnetically stirred for 7min at 300rpm, and then again at 5000rpm Use a homogenizer to homogenize for 4 minutes, emulsify for 15 seconds, and pause for 10 seconds to prepare the primary emulsion W 1 / O;
[0035] Preparation of β-cyclodextrin aqueous solution: Accurately weigh 3.5g of β-cyclodextrin, add it to 96.5g of deionized water, so that the mass concentration of β-cyclodextrin is 3.5%, and magnetically stir in a water bath at 70°C for 13 minutes , cooled to room temperature;
[0036] W 1 / O / W 2 P...
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