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pcr hot cover assembly and pcr instrument

A thermal cover and component technology, applied in instruments, optical components, biochemical instruments, etc., can solve the problems of large volume and complex structure of PCR instruments, and achieve the effects of reducing costs, improving accuracy, and improving optical stability.

Active Publication Date: 2020-11-24
HANGZHOU BIOER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a PCR hot cover assembly and a PCR instrument to alleviate the technical problems that although the existing hot cover has the function of preventing the test solution from evaporating, its arrangement with the optical system makes the PCR instrument larger in size and complex in structure.

Method used

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  • pcr hot cover assembly and pcr instrument
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  • pcr hot cover assembly and pcr instrument

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Embodiment Construction

[0043] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0044] Although the heat covers of most existing PCR instruments have the function of preventing the test solution from evaporating, since the heat covers are covered on the PCR orifice plate and are closely attached to the test tubes of the samples to be tested, during the PCR detection process, the optical system of the PCR instrument The emitted light needs to be irradiated on the tested sample test tube on the PCR well plate, so how to arrange the structure between the thermal cover, the optical system and the PCR wel...

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Abstract

The invention provides a PCR heat cover assembly and a PCR instrument, which relate to the technical field of biological detection and include: a heat cover base, an optical fiber positioning seat is arranged on the heat cover base, an optical fiber is installed on the optical fiber positioning seat; A lens fixing plate and a heating module are provided; a through hole corresponding to the position of the optical fiber is arranged on the lens fixing plate, and a lens is installed on the lens fixing plate, and the through hole can be aligned with the sample hole on the sample module. The invention integrates the thermal cover with the optical system including the optical fiber and the lens, so that the thermal cover can not only prevent the evaporation of the test solution, but also carry the optical system, which reduces the volume of the PCR instrument and reduces its structural complexity.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a PCR heat cover assembly and a PCR instrument. Background technique [0002] Real-time fluorescence quantitative polymerase chain reaction (Polymerase Chain Reaction, PCR) technology can realize the quantitative analysis of DNA template, which is of great significance to molecular biology research and medical research. Accompanied by rapid changes in the ambient temperature during the PCR gene amplification process, severe evaporation of the reaction reagents is inevitable, which greatly affects the normal progress of the reaction and the judgment of the results. In order to overcome the evaporation phenomenon in PCR gene amplification, hot lid technology is widely used. [0003] Although the heat covers of most existing PCR instruments have the function of preventing the test solution from evaporating, since the heat covers are covered on the PCR orifice plate and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38G02B7/02
CPCB01L7/52B01L3/50851G02B7/02
Inventor 曹进涛李冬贺贤汉
Owner HANGZHOU BIOER TECH CO LTD
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