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Preparation method of monodisperse high-load hydrophilic ion exchange polymethacrylate microspheres

A technology of polymethacrylate and polymethacrylic acid, which is applied in the direction of cation exchange materials, can solve problems such as limiting the scope of application, and achieve the effects of stable physical and chemical properties, simple cleaning and regeneration, and improved selectivity

Active Publication Date: 2020-06-26
无锡瑞格生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003]At present, most of the ion exchange media used in the field of biological protein analysis and purification are agarose, silica and other surface hydrophilic media, and there are also a small amount of polystyrene- Divinylbenzene and polyacrylate type media, but few of them use polysuccinimide on the surface of microspheres to make synthetic hydrophilic ion exchange media. The US20130240444 patent of polyLC company in the United States is to modify polysuccinimide into On the porous silica gel microspheres, a series of ion-exchange silica gel media can be obtained by ring-opening with amine reagents, but silica gel cannot be used under alkaline conditions with a pH greater than 8.5, which limits its application range

Method used

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  • Preparation method of monodisperse high-load hydrophilic ion exchange polymethacrylate microspheres
  • Preparation method of monodisperse high-load hydrophilic ion exchange polymethacrylate microspheres
  • Preparation method of monodisperse high-load hydrophilic ion exchange polymethacrylate microspheres

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Embodiment 1

[0024] Such as figure 1 As shown, a preparation method of monodisperse high-capacity hydrophilic ion-exchange polymethacrylate microspheres, first, the porous polyglycidyl methacrylate microspheres are aminated; then grafted polysuccinyl The amine undergoes a ring-opening reaction, and finally a tentacle-type ion-exchange group is obtained through a radical-initiated reaction with an olefinic monomer, thereby obtaining a hydrophilic high-loading polymethacrylate microsphere.

[0025] The steps of the preparation method are as follows:

[0026] Step 1, adding monodisperse porous polyglycidyl methacrylate microspheres into the reaction kettle, adding a solvent and ultrasonically mixing, then adding an amine reagent and mechanically stirring until uniformly mixed, and reacting at a constant temperature for 12 hours to obtain aminated microspheres; The mass volume (g / mL) ratio of described microsphere and solvent is 0.1, and described solvent adopts the mixed solution of water an...

Embodiment 2

[0033] A method for preparing monodisperse high-capacity hydrophilic ion-exchange polymethacrylate microspheres. First, porous polyglycidyl methacrylate microspheres are subjected to amination treatment; Ring reaction, and finally the tentacle-type ion exchange group is obtained through the free radical reaction with olefinic monomers, so as to obtain hydrophilic high-load polymethacrylate microspheres.

[0034] The steps of the preparation method are as follows:

[0035] Step 1, adding monodisperse porous polyglycidyl methacrylate microspheres into the reaction kettle, adding a solvent and ultrasonically mixing, then adding an amine reagent and mechanically stirring until uniformly mixed, and reacting at a constant temperature for 24 hours to obtain aminated microspheres; The mass volume (g / mL) ratio of described microsphere and solvent is 0.5, and described solvent adopts the mixed liquor of water and methanol, and the mass ratio of water and methanol is 1:3, and described a...

Embodiment 3

[0042] A method for preparing monodisperse high-capacity hydrophilic ion-exchange polymethacrylate microspheres. First, porous polyglycidyl methacrylate microspheres are subjected to amination treatment; Ring reaction, and finally the tentacle-type ion exchange group is obtained through the free radical reaction with olefinic monomers, so as to obtain hydrophilic high-load polymethacrylate microspheres.

[0043] The steps of the preparation method are as follows:

[0044] Step 1, adding monodisperse porous polyglycidyl methacrylate microspheres into the reaction kettle, adding a solvent and ultrasonically mixing, then adding an amine reagent and mechanically stirring until uniformly mixed, and reacting at a constant temperature for 18 hours to obtain aminated microspheres; The mass volume (g / mL) ratio of described microsphere and solvent is 0.3, and described solvent adopts the mixed liquor of water and methanol, and the mass ratio of water and methanol is 1:3, and described a...

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Abstract

The invention belongs to the field of biological separation and purification, and particularly relates to a preparation method of monodisperse high-load hydrophilic ion exchange polymethacrylate microspheres, which comprises the following steps: carrying out amination treatment on porous polyglycidyl methacrylate microspheres; then grafting polysuccinimide to carry out ring-opening reaction, and finally initiating reaction with free radicals of olefin monomers to obtain tentacle type ion exchange groups, so as to obtain the hydrophilic high-load polymethacrylate microspheres. According to theinvention, a hydrophilic covering layer similar to a polypeptide structure is formed on the surface of the porous microsphere; compared with the prior art, the composite material has good biocompatibility, and can effectively avoid most of non-specific adsorption, mainly hydrophobic adsorption, in the field of separation and purification, so that the composite material can only generate cation exchange with biomacromolecules, thereby enhancing the purification or separation selectivity.

Description

technical field [0001] The invention belongs to the field of bioseparation and purification, relates to a bioseparation and purification material, in particular to a method for preparing monodisperse high-capacity hydrophilic ion-exchange polymethacrylate microspheres. Background technique [0002] Ion exchange material is a natural or artificial organic or inorganic solid material that can extract ions from solution or slurry. These materials have the functions of ion adsorption, desorption, and ion exchange, and have the functions of H + , Na + , K + These cation exchange materials have OH - , Cl - , SO 4 2- These anions are exchanged with the material. If these groups with ion exchange functions are combined with the resin, they are called ion exchange resins or simply called cation resins and anion resins. [0003] At present, most of the ion exchange media used in the field of biological protein analysis and purification are surface hydrophilic media such as aga...

Claims

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Application Information

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IPC IPC(8): B01J39/20
CPCB01J39/20
Inventor 刘晓庆
Owner 无锡瑞格生物科技有限责任公司
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