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Photodynamic sterilization method for acidic foods

A photodynamic and food technology, applied in the preservation of food ingredients as anti-microbial, food preservation, food ingredients containing organic compounds, etc., can solve the problems of easy loss of antibacterial activity, limited application, and inability to apply in food fields, and achieve sterilization effect. Good, low cost, mild sterilization effect

Active Publication Date: 2020-06-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since photodynamic sterilization is a method of inactivating microorganisms by using photosensitizer molecules to absorb specific wavelengths of light to generate reactive oxygen species (ROS) with high oxidative capacity, photosensitizers are one of the key factors for photodynamic antibacterial. Currently, researchers The developed photosensitizers (Yang, Su-Geun, Biomaterials Research 2018, 22(1), 25) are mainly used in the fields of clinical cancer treatment or inflammation treatment. These photosensitizers usually have certain toxicity and cannot be used in the food field
In addition, most photosensitizers are easy to lose antibacterial activity under acidic conditions due to the protonation sites such as N and O, which limits their application in acidic foods.

Method used

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  • Photodynamic sterilization method for acidic foods
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  • Photodynamic sterilization method for acidic foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3、 comparative example 1

[0032] (1) Select fresh passion fruit, wash the outer skin and squeeze the juice, and filter the obtained juice for later use;

[0033] (2) the erythrosine photosensitizer with a purity of more than 95% is dissolved in purified water at room temperature to prepare a high-concentration erythrosine stock solution of 880 mg / kg;

[0034] (3) adding the erythrosine photosensitizer solution prepared by the final concentration of erythrosine to 8.8 mg / kg, and making it evenly distributed in the passion fruit juice;

[0035] (4) Put the passion fruit juice mixed with the erythrosine photosensitizer solution under the irradiance of 0, 6.0, 8.5, 11.0mW / cm 2 Under the green LED light (wavelength is 520-550nm) light, the light time is 10min, then seal and pack immediately, see the following table 1.

[0036] (5) Bacterial culture: test according to the determination method of the total number of bacterial colonies stipulated in the national standard (GB 4789.2-2016). The results of the ...

Embodiment 4-8、 comparative example 2

[0040] (1) Select fresh passion fruit, wash the outer skin and squeeze the juice, and filter the obtained juice for later use;

[0041] (2) the erythrosine photosensitizer with a purity of more than 95% is dissolved in purified water at room temperature to prepare a high-concentration erythrosine stock solution of 880 mg / kg;

[0042](3) adding the erythrosine photosensitizer solution prepared by the final concentration of erythrosine to 8.8 mg / kg, and making it evenly distributed in the passion fruit juice;

[0043] (4) Put the passion fruit juice mixed with erythrosine photosensitizer solution under irradiance of 100mW / cm 2 Illumination is carried out under a xenon lamp lamp, and the illumination time is 0, 1, 2, 5, 10, 20 minutes, and then sealed and packaged immediately, see Table 2 below.

[0044] (5) Bacterial culture: test according to the determination method of the total number of bacterial colonies stipulated in the national standard (GB 4789.2-2016). The results of...

Embodiment 9-13

[0048] (1) Select fresh passion fruit, wash the outer skin and squeeze the juice, and filter the obtained juice for later use;

[0049] (2) the erythrosine photosensitizer with a purity of more than 95% is dissolved in purified water at room temperature to prepare a high-concentration erythrosine stock solution of 880 mg / kg;

[0050] (3) Add the prepared erythrosine photosensitizer solution according to the final concentration of erythrosine as 4.4, 8.8, 17.5, 35.1, 43.9 mg / kg, and make it evenly distributed in the passion fruit juice, see Table 3 below;

[0051] (4) Put the passion fruit juice mixed with erythrosine photosensitizer solution under irradiance of 100mW / cm 2 Illumination was performed under a xenon lamp for 10 minutes, and then immediately sealed and packaged.

[0052] (5) Bacterial culture: test according to the determination method of the total number of bacterial colonies stipulated in the national standard (GB 4789.2-2016). The results of the total number o...

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Abstract

The invention discloses a photodynamic cold sterilization method for acidic foods. The method comprises the following steps: firstly preparing a stock solution with a high concentration of >=800 mg / kgfrom an erythrosine photosensitizer, adding the stock solution into the acidic foods according to a dose of <45 mg / kg, performing uniform distribution, finally putting the acidic foods mixed with theerythrosine photosensitizer solution under a visible light source for irradiation, and performing immediate sealed package. The method provided by the invention has mild sterilization conditions andlow costs, does not destroy nutrient substances and flavor in the acid foods (because of cold sterilization technology), and has a good sterilization effect after light irradiation, the inactivation rate of microorganisms in the treated products are all >90%, and the method prolongs the shelf life (>15 days), has no toxic effects on human bodies, and is a novel safe, efficient low-cost and easy-to-operate sterilization method.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a photodynamic sterilization method for acidic food. Background technique [0002] With the improvement of people's living standards and the increasing health problems related to diet, more and more people pay more attention to the nutritional value of food while pursuing the taste. Acidic foods (fruit and vegetable products, such as fruit and vegetable juices, pickles, etc.) are favored by people because of their good taste and high nutritional value, and their sales are also increasing year by year. [0003] As food to be sold on the shelves requires a certain period of sales, it needs to be sterilized during the production process. Traditional sterilization methods are mainly thermal sterilization (such as pasteurization and ultra-high temperature sterilization), that is, heat treatment methods that use steam and hot water as heat transfer media to transfe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/42A23L3/26A23L3/3508
CPCA23L2/42A23L3/3508A23L3/26A23V2002/00A23V2200/10A23V2250/30
Inventor 吴鹏王颖蒋小明侯贤灯
Owner SICHUAN UNIV
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