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EPA extract and preparation method and application thereof

An extract and extract technology, applied in the field of EPA extract and its preparation, can solve the problems of high protein and pigment residues, complicated purification steps, low EPA content, etc. Effect

Pending Publication Date: 2020-06-16
GUOTOU BIO TECH INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing methods for extracting EPA from microalgae raw materials usually rely on the action of enzymes, the extraction efficiency is low, the content of EPA is low, and there are many protein and pigment residues in the extract, which requires complicated purification steps in the later stage. Downstream products

Method used

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  • EPA extract and preparation method and application thereof
  • EPA extract and preparation method and application thereof
  • EPA extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] This example is used to illustrate the method for extracting fatty acids from algae raw materials provided by the present invention

[0058] (1) Add the spherical silica beads (about 1mm in diameter) and the Xanthellae algae powder into the extraction tube, add the sulfuric acid-methanol solution to shake and mix, wherein the dosage of the spherical silica beads is 0.3 relative to 1g of the Xanthellae algae powder. g, the amount of sulfuric acid-methanol solution is 30 mL, and the concentration of sulfuric acid in the sulfuric acid-methanol solution is 3% by weight. After that, the mixture was placed in a rapid nucleic acid extractor, and the oscillation speed was set to 5m / s, the running time was 20s / cycle, and the wall-breaking cycle was run 8 times at room temperature to obtain the lysate.

[0059] (2) Add n-hexane-ethanol (volume ratio 2:1) to the lysate. The amount of n-hexane-ethanol added is 4 mL relative to 1 g of Xanthophyllum algae powder. Centrifuge the mixture at...

Embodiment 2

[0062] This example is used to illustrate the method for extracting fatty acids from algae raw materials provided by the present invention

[0063] (1) Add spherical silica beads (about 0.5mm in diameter) and Xanthellae algae powder into the extraction tube, add sulfuric acid-methanol solution and shake and mix, wherein the amount of spherical silica beads relative to 1g of Xanthellae algae powder is 0.4g, the amount of sulfuric acid-methanol solution is 20 mL, and the concentration of sulfuric acid in the sulfuric acid-methanol solution is 1% by weight. After that, the mixture was placed in a rapid nucleic acid extractor, and the oscillation speed was set to 4m / s, the running time was 40s / cycle, and the wall-breaking cycle was run 8 times at room temperature to obtain the lysate.

[0064] (2) Add n-hexane-ethanol (volume ratio 1.5:1) to the lysate. Relative to 1g of Xanthophyllum powder, the amount of n-hexane-ethanol added is 10mL. Centrifuge the mixture at room temperature to ob...

Embodiment 3

[0067] This example is used to illustrate the method for extracting fatty acids from algae raw materials provided by the present invention

[0068] (1) Add spherical silica beads (about 1.5mm in diameter) and Xanthellae algae powder into the extraction tube, add sulfuric acid-methanol solution and shake and mix evenly, wherein the amount of spherical silica beads relative to 1g of Xanthellae algae powder is 0.5 g, the amount of sulfuric acid-methanol solution is 40 mL, and the concentration of sulfuric acid in the sulfuric acid-methanol solution is 5% by weight. After that, the mixture was placed in a rapid nucleic acid extractor, and the oscillation speed was set to 6m / s, the running time was 60s / cycle, and the wall-breaking cycle was run 8 times at room temperature to obtain the lysate.

[0069] (2) Add n-hexane to the lysate. The amount of n-hexane added is 20 mL relative to 1 g of Xanthophyllum algae powder. Centrifuge the mixture at room temperature to take the upper extract i...

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Abstract

The invention relates to the field of EPA extraction, in particular to an EPA extract and a preparation method and application thereof. The method comprises the following steps: (1) in the presence ofa grinding medium, contacting an alga raw material with an extracting solution and / or a phase splitting solvent, and conducting oscillating to realize wall breaking treatment; and (2) in the presenceof the phase splitting solvent, carrying out split-phase extraction on a wall-broken product obtained in the step (1) to obtain a first raffinate phase and a first extraction phase, thereby obtaininga fatty acid-rich extraction phase containing the first extraction phase. According to the method, the purpose of improving the extraction efficiency of algal EPA can be achieved, and the extract with low protein and pigment content and high EPA content can be obtained.

Description

Technical field [0001] The invention relates to the field of extraction of EPA, in particular to an EPA extract and a preparation method and application thereof. Background technique [0002] Eicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid, which is an essential fatty acid that the body cannot synthesize and must be taken from food. It is closely related to the physiological functions of the human body. It can maintain the normal function and growth and development of the brain and retina. It has the effects of inhibiting platelet aggregation, lowering blood lipids, preventing arteriosclerosis, strengthening the brain, protecting vision, and improving immunity. Inflammation, some cancers, and diabetes are also effective. At present, it is widely used in various health products, milk powder, dairy products and various bakery products. [0003] Land plants and marine organisms are the main sources of EPA in nature. Microalgae are also a major source of EPA, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23K10/30A61K36/02A61P29/00A61P3/04A61P3/10
CPCA23L33/105A23K10/30A61K36/02A61P29/00A61P3/04A61P3/10A23V2002/00A23V2200/30A23V2200/328A23V2200/332A23V2250/202A23V2300/14
Inventor 加晶杨小红喻正保韩丹翔楚平广胡强
Owner GUOTOU BIO TECH INVESTMENT CO LTD
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