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Antigen stabilizer

A stabilizer and antigen technology, applied in the field of biochemical analysis, can solve the problems of poor antigen storage stability, no thermal stability and transportation stability, and achieve good thermal stability and transportation stability, stable activity, and cost reduction. Effect

Pending Publication Date: 2020-06-12
SUZHOU DIAGVITA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an antigen stabilizer that can solve the aforementioned problems in view of the existing antigen stabilizers described in the background technology, which have poor storage stability for low-concentration antigens, and do not have thermal stability and transport stability. agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An antigen stabilizer comprising a buffer, a stabilizer, a surfactant and a preservative;

[0018] Buffer is 2-(N-morpholine) ethanesulfonic acid aqueous solution, and its concentration is 20mmol / L;

[0019] Stabilizers include inorganic salts: 2%, metal complexing agents: 2%, antioxidants: 2%, trehalose: 1% and sucrose: 2.5%;

[0020] The surfactant is Tween-20: 0.03%;

[0021] The preservative is methylchloroisothiazolinone: 0.05%;

[0022] The percentages of the above-mentioned components are all percentages by mass.

[0023] The antigen stabilizer of the present embodiment is used for the assay value of antigen and the comparative data of existing stabilizer as follows:

[0024]

[0025]

Embodiment 2

[0027] An antigen stabilizer comprising a buffer, a stabilizer, a surfactant and a preservative;

[0028] The buffer solution is an aqueous solution of 4-hydroxyethylpiperazineethanesulfonic acid, and its concentration is 50mmol / L;

[0029] Stabilizers include inorganic salts: 1%, antioxidants: 5%, trehalose: 0.8% and sucrose: 5%;

[0030] The surfactant is Tween-80: 0.01%;

[0031] Preservatives for antimicrobial peptides: 0.1%

[0032] The percentages of the above-mentioned components are all percentages by mass.

[0033] The antigen stabilizer of the present embodiment is used for the assay value of antigen and the comparative data of existing stabilizer as follows:

[0034]

[0035]

Embodiment 3

[0037] An antigen stabilizer comprising a buffer, a stabilizer, a surfactant and a preservative;

[0038] Buffer is 2-(N-morpholine) ethanesulfonic acid aqueous solution, and its concentration is 40mmol / L;

[0039] Stabilizers include metal complexing agent: 5%, antioxidant: 1%, trehalose: 0.2% and sucrose: 1%;

[0040] The surfactant is brij35: 0.03%;

[0041] The preservative is methylisothiazolinone: 0.05%;

[0042] The percentages of the above-mentioned components are all percentages by mass.

[0043] The antigen stabilizer of the present embodiment is used for the assay value of antigen and the comparative data of existing stabilizer as follows:

[0044]

[0045]

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PUM

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Abstract

The invention discloses an antigen stabilizer. The antigen stabilizer comprises a buffer solution, a stabilizer, a surfactant and a preservative, the concentration of the buffer solution is 20-200 mmol / L, the mass percent of the stabilizer is 1%-26%, the mass percent of the surfactant is 0.01%-0.15%, and the mass percent of the preservative is 0.001%-0.46%. The buffer solution is prepared from oneor two of 100mM of 2-(N-morpholine) ethanesulfonic acid and 100mM of 4-hydroxyethyl piperazine ethanesulfonic acid, the stabilizer comprises one or more of inorganic salt, a metal complexing agent, an antioxidant, trehalose, cane sugar and glycerol, the surfactant is prepared from one or more of tween-20, tween-80 and brij35, and the preservative comprises one or more of antibacterial peptide, methyl isothiazolinone, methyl isothiazolinone and polylysine. According to the antigen stabilizer, the antigen can be stably stored for a long time at the low concentration of 20 ng / ml or below, the activity of the antigen is basically stable, the antigen has better thermal stability and transportation stability, a product is more convenient to transport and store, and the transportation and storage cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to an antigen stabilizer. Background technique [0002] Calibrators and quality controls of in vitro diagnostic reagents are important tools to achieve accurate and consistent results of clinical testing and supervisory testing of in vitro diagnostic reagents. play a very important role in the inspection. [0003] Many of the main components included in the calibration quality control are biologically active antigenic proteins. The protein itself is easy to degrade when stored for a long time, or its functional activity changes due to changes in the storage environment. The patent document with the publication number CN106483282B discloses an antigen stabilizer, specifically disclosed, including the following components by weight, 100 parts of Tris-HCl buffer solution, 1-5 parts of bovine serum albumin, and 1-10 parts of glycerol 1-10 parts of mannitol, 0.1-0.5 parts ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 贾引军金君玉赵年福王明伟张辉吴一凡
Owner SUZHOU DIAGVITA BIOTECH
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