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Injectable hyaluronic acid hydrogel for cartilage repair and preparation method of injectable hyaluronic acid hydrogel

A technology for hyaluronic acid and cartilage repair, which is used in pharmaceutical formulations, medical science, prostheses, etc. It can solve the problems of low inducibility of biomaterials, high risk of immune rejection, and high treatment costs, optimize graft concentration, and reduce surgery. Number of times, significant social and economic effects

Inactive Publication Date: 2020-06-09
GUANGDONG GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Therefore, in order to be able to simultaneously solve the deficiencies in the current practice of cartilage repair, that is, low inductivity of biomaterials, high risk of immune rejection, low effectiveness and high cost of treatment, in addition to considering the selection and preparation process of biomimetic scaffold materials, it is also necessary to consider Design and improvement of its molecular weight, cross-linking degree, curing performance, degradation performance, mechanical properties, etc.

Method used

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  • Injectable hyaluronic acid hydrogel for cartilage repair and preparation method of injectable hyaluronic acid hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Step 1: dissolving sodium hyaluronate in water at room temperature to form a transparent solution with a mass fraction of 0.1%, adding a cation exchange resin and stirring overnight, and filtering to obtain a hyaluronic acid stock solution;

[0067] Step 2 Add periodate to the hyaluronic acid stock solution obtained in step 1, the molar ratio of the two is 10:1, place the system in the dark for 0.5 hours; add ethylene glycol to remove excess oxidant, and continue stirring for 1 hour After the reaction is terminated, the product is purified by dialysis and freeze-dried to obtain ring-opened hyaluronic acid;

[0068] Step 3: Add cross-linking agent EDC / NHS to the hyaluronic acid stock solution obtained in step 1, the molar ratio is 1:1, stir for 0.5 hours, add excess hydrazine to react for 24 hours; dialysis purification, freeze-drying to obtain hyaluronic acid grafted hydrazide acid;

[0069] Step 4 Dissolve the above two modified hyaluronic acids in PBS solution respec...

Embodiment 2

[0071] Step 1: Dissolve sodium hyaluronate in water at room temperature to form a transparent solution with a mass fraction of 3%, add cation exchange resin and stir overnight, and filter to obtain a hyaluronic acid stock solution;

[0072] Step 2 Add permanganate to the hyaluronic acid stock solution obtained in step 1, the molar ratio of the two is 1:10, and place the system in a dark place to react for 6 hours; add ethylene glycol to remove excess oxidant, and continue stirring for 1 hour After the reaction is terminated, the product is purified by dialysis and freeze-dried to obtain ring-opened hyaluronic acid;

[0073] Step 3: Add cross-linking agent EDC / NHS to the hyaluronic acid stock solution obtained in step 1, the molar ratio is 1:10, stir for 0.5 hours, add excess adipic dihydrazide, react for 24 hours; dialysis purification, freeze-drying to obtain graft hydrazide hyaluronic acid;

[0074] Step 4 Dissolve the above two modified hyaluronic acids in PBS solution res...

Embodiment 3

[0076] Step 1: dissolving sodium hyaluronate in water at room temperature to form a transparent solution with a mass fraction of 0.1%, adding a cation exchange resin and stirring overnight, and filtering to obtain a hyaluronic acid stock solution;

[0077] Step 2 Add methacrylic anhydride in an equal mass ratio to the hyaluronic acid stock solution obtained in step 1, maintain the pH of the system at 8, and react at 4°C for 12 hours to obtain hyaluronic acid modified with methacrylic acid; then add hyaluronic acid The pH of the system was adjusted to 6.0, the cross-linking agent EDC / NHS was added to the solution, stirred for 0.5 hours, the TGF-β affinity peptide HSNGLPL was added, reacted for 3 hours, purified by dialysis, and freeze-dried to obtain the grafted TGF-β affinity peptide Polypeptide hyaluronic acid.

[0078] Step 3 Add chlorate to the hyaluronic acid stock solution obtained in step 1, the molar ratio of the two is 10:1, and place the system in a dark place to reac...

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Abstract

The invention relates to a medical material, in particular relates to the field of cartilage damage regeneration and mainly discloses an injectable hyaluronic acid hydrogel for cartilage repair and apreparation method of the injectable hyaluronic acid hydrogel. The hyaluronic acid hydrogel product provided by the invention mainly aims at cases that cartilage defect shapes are irregular and conventional regenerated cartilage is not well combined with host cartilage, can be injected, can be spontaneously formed as a gel in a liquid environment, is good in adhesiveness, and achieves seamless combination of cartilage defect parts and filling materials.

Description

technical field [0001] The invention relates to a medical material, in particular to the field of cartilage injury regeneration, and mainly discloses an injectable hyaluronic acid hydrogel for cartilage repair and a preparation method thereof. Background technique [0002] Osteochondral defects in joints caused by trauma or bone disease are common in clinical practice and seriously affect the quality of life of patients. It has become one of the main causes of physical disability. Knee disease can occur in people of all ages. Cartilage defects in the knee can result from trauma, sprains, overuse of the knee, muscle weakness, or general wear and tear. Due to the special tissue structure and biological characteristics of articular cartilage, although many methods are known to treat articular cartilage defects, including but not limited to: subchondral bone drilling, electrical stimulation, laser, drug and cell injection, gene therapy, etc., However, none of the above methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/50A61L27/20A61L27/22A61L27/54C08J3/075C08L5/08C08B37/08
CPCA61L27/20A61L27/227A61L27/50A61L27/52A61L27/54A61L2300/414A61L2400/06A61L2430/06C08B37/0072C08J3/075C08J2305/08C08J2405/08C08L5/08C08L89/00
Inventor 郑秋坚邓展涛马元琛李梦远郑铭豪
Owner GUANGDONG GENERAL HOSPITAL
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