Method for constructing rat model of non-diabetes-based severe non-alcoholic chronic steatohepatitis

A steatohepatitis and non-alcoholic technology, applied in application, animal feed, additional food elements, etc., can solve the problems of long time, not obvious pathological changes of NASH, NASH model does not have the characteristics of liver fibrosis, etc., and achieve no death High efficiency and good stability

Inactive Publication Date: 2020-06-09
凯斯艾生物科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reagents used by DEN to induce liver cancer in mice and rats take a long time, more than 36 weeks, and the pathological changes of NASH are not obvious enough
[0005] Currently applied NASH models do not have the characteristics of moderate to severe liver fibrosis

Method used

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  • Method for constructing rat model of non-diabetes-based severe non-alcoholic chronic steatohepatitis
  • Method for constructing rat model of non-diabetes-based severe non-alcoholic chronic steatohepatitis
  • Method for constructing rat model of non-diabetes-based severe non-alcoholic chronic steatohepatitis

Examples

Experimental program
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Embodiment 1

[0025] Example 1 Preparation of Severe Nonalcoholic Steatohepatitis Rat Model on Non-diabetic Basis

[0026] Taking SD male rats as an example, a method for making an animal model of severe non-alcoholic steatohepatitis (NASH) with moderate liver fibrosis on a non-diabetic basis is provided, including the following steps:

[0027] Six pregnant SD female mice were selected, with a 12-h light and night cycle, a temperature of 22°C±2°C, and a humidity of 50%-60%.

[0028] (1) Five male pups were not treated and fed with breastmilk normally.

[0029] (2) Five male pups were given a single subcutaneous injection of normal saline two weeks after birth.

[0030] (3) Other male pups were given a single subcutaneous injection of DEN (50mg / kg) 2 weeks after birth.

[0031] (4) After breastfeeding (4 weeks after birth), the male pups were divided into cages and grouped:

[0032] Naive-12wk group: male animals without DEN injection, fed with normal maintenance feed for 8 weeks;

[0033]...

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Abstract

The invention discloses a method for constructing a rat model of non-diabetes-based severe non-alcoholic chronic steatohepatitis. Rats are given a single injection of diethylnitrosamine in two weeks after birth, and fed with high-fat and high-cholesterol cholate feeds for 8-12 consecutive weeks from four weeks after birth to construct the rat model of severe non-alcoholic chronic steatohepatitis accompanied with moderate chronic hepatic fibrosis. The model has the characteristic of severe non-alcoholic chronic steatohepatitis (NASH) accompanied with moderate chronic hepatic fibrosis; induced animals have severe fatty changes, severe inflammatory lesions and ballooning degeneration of aggravating hepatocyte apoptosis in hepatic tissue; and the rat model generates a basic pathological process of the severe NASH, especially a pathologic change accompanied with significant hepatic fibrosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rat model of severe non-alcoholic chronic steatohepatitis on a non-diabetic basis and its construction method and application. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is one of the most common clinical liver diseases, among which non-alcoholic steatohepatitis (NASH) is the liver, NAFL) to non-alcoholic fatty liver cirrhosis is a very important link, is one of the important causes of cryptogenic liver cirrhosis. Studies have shown that the prognosis of NASH is poor. About 50% of NASH patients will develop into liver fibrosis after 6 years of follow-up, 10%-15% will develop into liver cirrhosis within 10-20 years, and 9%-26% will develop into liver cirrhosis after follow-up. within 4-10 years of death due to end-stage liver disease. Therefore, research on the prevention and treatment strategies of NASH is one of the hot spots in the field of liver di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K20/158A23K20/22A23K50/50
CPCA01K67/02A23K50/50A23K20/158A23K20/22
Inventor 杨祖经陈继巧庄永杰
Owner 凯斯艾生物科技(苏州)有限公司
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