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Panax japonicus glycosyltransferase UGTPjm2 and application thereof to preparation of panax japonicus sapinogenin IVa

A technology of ginseng saponins and ginseng sugar bases is applied in the field of bioengineering, and can solve the problems of unclear synthesis path of ginseng saponins IVa, affecting the progress of biosynthesis of ginseng saponins IVa and the like.

Active Publication Date: 2020-06-05
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, the synthesis route of bamboo ginseng saponin IVa is not clear and has not been verified, thus affecting the advancement of the biosynthesis of bamboo ginseng saponin IVa

Method used

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  • Panax japonicus glycosyltransferase UGTPjm2 and application thereof to preparation of panax japonicus sapinogenin IVa
  • Panax japonicus glycosyltransferase UGTPjm2 and application thereof to preparation of panax japonicus sapinogenin IVa
  • Panax japonicus glycosyltransferase UGTPjm2 and application thereof to preparation of panax japonicus sapinogenin IVa

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Embodiment 1

[0034] The preparation of embodiment 1 pearl ginseng glycosyltransferase UGTPjm2

[0035] The glycosyltransferase UGT74M1 identified in Saponaria vaccaria, the glycosyltransferase UGT73F3 identified in Medicago truncatula (Medicago truncatula), and the glycosyl identified in Centella asiatica (L.) Urban Using the transferase UGT73AH1 as a clue, the 59 glycosyltransferases (length greater than 900 bp) contained in Ginseng ginseng and the amino acid sequences of the above three glycosyltransferases were analyzed in a phylogenetic tree, and the phylogenetic tree was constructed using MEGA 6 software.

[0036] On the basis of the constructed phylogenetic tree, combined with the comprehensive analysis of the content characteristics of ginseng saponin IVa and gene expression, the candidate genes that may be involved in the biosynthesis of ginsenoside IVa were preliminarily screened out. After a series of work such as cDNA preparation, candidate gene amplification and recovery, homol...

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Abstract

The invention discloses panax japonicus glycosyltransferase UGTPjm2 and an application thereof to preparation of panax japonicus sapinogenin IVa. The panax japonicus glycosyltransferase UGTPjm2 has anamino acid sequence as shown in SEQ ID No.1. The panax japonicus glycosyltransferase UGTPjm2 disclosed by the invention can catalyze glycosylation of C-28 site carboxyl groups of oleanolic acid-3-O-beta-glucuronide, and further, the panax japonicus sapinogenin IVa is generated. An important way is provided for artificial production of the panax japonicus sapinogenin IVa.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a ginseng glycosyltransferase UGTPjm2 and its application in preparing ginseng saponin IVa. Background technique [0002] According to the types of ginsenosides contained in ginseng plants, ginseng plants can be divided into two categories, one is mainly dammarane-type saponins, such as ginseng (Panax. ), American ginseng (P.quinquefolius), etc., and the other type is mainly oleanane-type saponins, such as pearl ginseng (P.japonicus var.major), bamboo ginseng (P. P. japonicus var. angustifolius), P. zingiberensis, P. stipuleanatus, P. japonicus var. bipinnatifidus, etc. Bamboo ginseng saponin IVa is an oleanane-type saponin, and it is one of the components with the highest content in ginseng plants such as pearl ginseng, ginger-shaped notoginseng, and bamboo ginseng. It has been clearly stated in the 2015 edition of the Pharmacopoeia of the People's Republic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/70C12N1/21C12P19/56C12R1/19
CPCC12N9/1048C12N15/70C12P19/56
Inventor 杨生超唐军荣张广辉卢迎春唐卿雁陈庚林源李莹
Owner YUNNAN AGRICULTURAL UNIVERSITY
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