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Application of amomum maximum roxb extract in preparation of alpha-glucosidase inhibitor drugs

A technology of glucosidase and cardamom, applied in the field of medicinal chemistry, to achieve the effects of reducing production and absorption, protecting pancreatic function, and slowing blood sugar rise

Active Publication Date: 2020-06-05
广东省科学院南繁种业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the effect of some specific components in the extract of cardamom japonica on α-glucosidase

Method used

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  • Application of amomum maximum roxb extract in preparation of alpha-glucosidase inhibitor drugs
  • Application of amomum maximum roxb extract in preparation of alpha-glucosidase inhibitor drugs
  • Application of amomum maximum roxb extract in preparation of alpha-glucosidase inhibitor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 formula I compound

[0033] Take 2 kg of dry cardamom rhizomes and crush them, heat and reflux them with 5L of 95% ethanol for three times, combine the three extracts and concentrate under reduced pressure until there is no alcohol smell, add pure water until the total volume is 1L, and use an equal volume of ethyl acetate The ester was extracted three times, and the ethyl acetate extracts were combined and concentrated to dryness under reduced pressure.

[0034] The ethyl acetate extract was subjected to silica gel column chromatography with petroleum ether-acetone gradient elution. Collect petroleum ether-acetone 9:1 eluted fractions, and recover to dryness under reduced pressure. Then through silica gel column chromatography, dichloromethane-methanol gradient elution, collect 98:2 elution fraction, this fraction utilizes preparative liquid chromatography to carry out separation, as figure 1 Shown is the liquid chromatogram of the fra...

Embodiment 2

[0035] The preparation of embodiment 2 formula I compound

[0036]Take 2 kilograms of dried cardamom rhizomes and grind them, extract by percolation with 20L 95% ethanol, combine the alcohol extracts, concentrate under reduced pressure to about 2L, add 500 grams of pretreated macroporous resin (resin model can be D101, AB -8, HPD-100 and HP20, etc.). After fully volatilizing to remove ethanol, pack the column, ethanol-water gradient elution (0, 30%, 60%, 80% and 95% ethanol), the fraction eluted with 80% ethanol is the target fraction, concentrated to dryness under reduced pressure; Column chromatography, dichloromethane-methanol gradient elution, collect 98:2 elution fraction, the fraction is separated by preparative liquid chromatography, the mobile phase is methanol-water 85:15, the flow rate is 6ml / min, the chromatographic column is RP-C18 chromatographic column (10*250mm), the detection wavelength is set at 280nm, and the chromatographic peaks with a retention time of 18...

Embodiment 3

[0037] Embodiment 3 formula I compound structure identification

[0038] The structure of the compound of formula I was determined by nuclear magnetic resonance spectrum and mass spectrometry analysis.

[0039] Its carbon spectrum and hydrogen spectrum data are as follows:

[0040] 1 H-NMR (600MHz, CDCl3) δ7.67(d, J=6.0Hz, 1H), 7.56(s, 1H), 6.33(d, J=6.0Hz, 1H), 4.82(s, 1H), 4.64( s,1H),2.69(d,J=16.2Hz,1H),2.45(dd,J=11.4,16.2Hz,1H),2.40-2.36(m,1H),2.04-1.95(m,2H),1.89 -1.85(m,1H),1.78-1.73(m,1H),1.64-1.56(tq,J=3.0,13.8Hz,1H),1.55-1.48(m,1H),1.43-1.38(m,1H) ,1.37-1.32(dq,J=4.2,12.6Hz,1H),1.24-1.15(m,3H),0.88(s,3H),0.82(s,3H),0.78(s,3H).

[0041] 13 C-NMR (150MHz, CDCl3) δ178.70(-CO-,C-13), 154.61(=CH-,C-15), 152.99(=CH-,C-16), 147.70(C,C-8 ),129.85(C,C-12),116.36(=CH-,C-14),107.77(=CH-,C-17),55.54(CH,C-5),53.96(CH,C-9) ,42.06(CH2,C-3),39.98(C,C-10),39.01(CH2,C-1),38.07(CH2,C-7),33.63(CH3,C-18),33.63(C, C-4), 24.37(CH2,C-6), 21.72(CH3,C-19), 19.54(CH2,C-11), 19.36(CH2,C-...

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Abstract

The invention relates to the field of medicinal chemistry, and in particular to application of amomum maximum roxb extract in the preparation of alpha-glucosidase inhibitor drugs. Specifically, the invention relates to application of a compound shown by a formula I or pharmaceutically acceptable salt of the compound in the preparation of the alpha-glucosidase inhibitor drugs. The compound of the formula I is extracted and isolated from the complex chemical components of amomum maximum roxb, and alpha-glucosidase activity can be significantly inhibited.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to the application of cardamom japonicus extract in the preparation of α-glucosidase inhibitor drugs. Background technique [0002] α-glucosidase (α-glucosidase) mainly includes enzymes such as maltase, sucrase, isomerized maltase, and lactase, which are widely distributed in the brush border of the small intestinal villous mucosa cells and participate in the digestion of food, glycoprotein It is a general term for a class of enzymes that can catalyze the hydrolysis of α-glucose groups from the non-reducing ends of substrates containing α-glucosidic bonds. [0003] α-glucosidase affects the formation of complex carbohydrates on the cell surface, and diseases such as diabetes, obesity, hyperlipidemia, inflammation, cancer, immune response, and viral infection are closely related to the formation of complex carbohydrates on the cell surface, so α-glucosidase inhibitors not only hav...

Claims

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Application Information

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IPC IPC(8): A61K31/351A23L33/105A61P3/10A61P3/04A61P3/06A61P29/00A61P35/00A61P37/02A61P31/12
CPCA23V2002/00A61K31/351A23L33/105A61P3/04A61P3/06A61P3/10A61P29/00A61P31/18A61P35/00A61P37/02A23V2200/30A23V2200/328A23V2200/3262A23V2200/332A23V2200/308A23V2200/324A23V2250/21
Inventor 卢传礼刘娟李亚军
Owner 广东省科学院南繁种业研究所
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