Rhythmic photostimulation pattern system and application thereof
A light stimulation, rhythmic technology, applied in the field of biomedicine, to achieve the effect of improving osteoporosis, high safety and convenient operation
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Embodiment 1
[0045] Construction of a Nude Mouse Model of Osteoporosis Induced by Secondary Hyperparathyroidism
[0046] (1) Gland tissue culture of human secondary hyperparathyroidism:
[0047] Cut the parathyroid tissue from the patient with secondary hyperparathyroidism into 1mm pieces 3 Tissue culture for tissue blocks of different sizes, specifically: place the obtained hyperparathyroid tissue in pre-cooled D-Hanks solution, use fine forceps and surgical scissors to peel off the vascular tissue, and then carefully cut the tissue to 1mm 3 Size: Add culture medium (RPMI1640+10% FBS+1% double antibody) to the 6-well plate, then move the tissue block into it and culture it in a 37°C, 5% CO2 incubator. Subsequent virus infection / transplantation experiments were performed after 3-7 days of culture.
[0048] (2) Virus infection:
[0049] Dilute the lentiviral particles Lenti-CMV-ChETA-eYFP or Lenti-CMV-GFP with RPIM1640 medium without serum and double antibody at a concentration of 1:200 ...
Embodiment 2
[0053] Photostimulation test:
[0054] One week after the operation of the nude mice described in Example 1, the transplanted parathyroid glands were stimulated by blue light for four weeks, which were divided into two modes: (1) stimulated three times a week, each time for 30 minutes, and the light wavelength was 470nm , light frequency 20Hz, 50ms stimulation interval; (2) stimulation three times a week, each containing two 15min (15min stimulation in the morning, 15min stimulation in the afternoon), light wavelength 470nm, light frequency 20Hz, 50ms stimulation interval.
Embodiment 3
[0056] In this example, kits (PTH measurement: Mouse PTH ELISA Kit, LSBio, product number LS-F5549; blood calcium determination: Calcium Colormetric Assay sufficient for 250colorimetric tests, Sigma, product number MAK022) were used to test the nude mice in each group after the operation in Example 2. The changes of parathyroid hormone and blood calcium concentration in serum were monitored (for mode (1), blood samples were collected before stimulation, immediately after stimulation, 5min after stimulation, 15min after stimulation, and 30min after stimulation for PTH and blood Calcium analysis; for mode (2), blood samples were also collected before stimulation, immediately after stimulation, 5min after stimulation, 15min after stimulation, and 30min after stimulation for PTH and blood calcium analysis. The purpose is to compare and analyze the effects of different stimulation modes on Effects of PTH and Calcium).
[0057] The result is as Figure 1-Figure 6 shown (where figu...
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