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A kind of detection method of isocitrunate content in blood plasma

A technology of isoartin and detection method, which is applied in the field of detection of isoarin content in plasma, and can solve problems such as inability to quantitatively detect isoarin

Active Publication Date: 2021-05-11
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to research reports, methods such as liquid chromatography, ultraviolet spectroscopy, NMR, and liquid chromatography-mass spectrometry have been used for the detection of isoagarin in citrus extracts, but these methods cannot quantitatively detect isoaurin in biological samples

Method used

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  • A kind of detection method of isocitrunate content in blood plasma
  • A kind of detection method of isocitrunate content in blood plasma
  • A kind of detection method of isocitrunate content in blood plasma

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Experimental program
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Effect test

Embodiment 1

[0027] 1. Instruments and reagents

[0028] 1) Analytical instrument

[0029] The liquid mass spectrometer is an Agilent 1290UHPLC-6420 triple quadrupole mass spectrometer system, equipped with a 1290 quaternary pump, a 1290 autosampler, a 1260 column oven, an electrospray ion source and a MassHunter workstation. The chromatographic column is an Agilent ZORBAX Eclipse Plus C18 RRHD column.

[0030] 2) Reagent

[0031] Isoagarin and keratophyllin were provided by Chengdu Mansite Biotechnology Co., Ltd., and the content was greater than 99%. Methanol, acetonitrile and formic acid were chromatographically pure, other reagents were analytically pure, and water was prepared by a Millipore water purifier.

[0032] 2. Experimental methods and results

[0033] 1) Solution preparation

[0034] Preparation of standard solution: Accurately weigh about 1 mg of isoaurin standard product, and dissolve it in a certain amount of methanol to obtain 1 mg / mL isoaurin mother solution. Dilute...

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Abstract

The invention provides a method for detecting the content of isoatrienin in blood plasma, which belongs to the field of drug analysis. The present invention establishes a method for detecting isoartin in plasma based on precipitation method and liquid chromatography-mass spectrometry, which can be used to detect the concentration of isoartin in living organisms and provide reliable analytical means for clinical and basic research. The present invention uses protein Precipitation method is used to treat plasma samples containing isoaurin, and remove impurities and endogenous interference substances through a one-step precipitation reaction. During the separation process of high performance liquid chromatography, a certain amount of formic acid is added to the mobile phase to make isoaurin After that, more hydrogenated quasi-molecular ion peaks are formed, and the abundance of the corresponding characteristic fragment ions increases accordingly, which improves the detection sensitivity; The specific and high-sensitivity monitoring of molecular ion peak→product ion realizes the quantitative detection of isoagarin.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for detecting the content of isoaurin in blood plasma. Background technique [0002] Isoatrienone is a polymethoxylated flavone, which widely exists in a variety of citrus plants and has a variety of biological activities. Molecular formula is C 20 h 20 o 7 , with a molecular weight of 372.1. Isoaurinones have multiple pharmacological activities, including antitumor, antiproliferative, and antioxidant effects. In addition, there may be drug interactions between isoagarin and other drugs, and it can increase the plasma drug concentration of some drugs when taken at the same time. The research on polymethoxylated flavones represented by isoagarin is increasing day by day, and the determination of its plasma drug concentration in vivo plays an important role in evaluating drug efficacy and analyzing pharmacological effects. According to research reports, methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/04G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/04G01N30/06G01N30/34G01N30/7266G01N2030/027G01N2030/045G01N2030/067
Inventor 叶炜剑牛超孙佳陈根黄晓忠
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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