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Application of multi-type mixed cells in myocardial infarction cell treatment

A myocardial infarction, multi-type technology, applied in the field of biomedicine, can solve problems such as poor curative effect, and achieve the effects of improving cardiac function, reducing the interference of physical and chemical factors, and reducing myocardial area

Active Publication Date: 2020-05-22
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cell therapy has emerged as the times require. At present, it has been reported that there are single stem cells, fibroblasts, genes, extracellular matrix components, etc. for the treatment of myocardial infarction, or a combination of the above-mentioned cells, but the effect is minimal.

Method used

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  • Application of multi-type mixed cells in myocardial infarction cell treatment
  • Application of multi-type mixed cells in myocardial infarction cell treatment
  • Application of multi-type mixed cells in myocardial infarction cell treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Establishment of mouse arteriovenous transplantation animal model and acquisition of multi-type mixed cells

[0038] Both C57 mice and wild-type mice were kept in an animal room with constant temperature and humidity, and had free access to food and water. The terrarium was covered with soft sawdust to reduce the discomfort and stimulation of the animals. Fasting for 12h before the experiment, without water. The experimental conditions were all carried out at about 25°C.

[0039] C57 mice, male or female, weighing 30 g, were anesthetized by intraperitoneal injection of 1% pentobarbital at a concentration of 0.1 mL / 10 g. Fix in supine position, fully expose the sterilized surgical field of the neck, make a midline incision, and bluntly dissect the tissue around the right common carotid artery of the recipient mouse. The chest cavity of the donor mouse was opened, a window was opened in the right atrium of the mouse, and heparinized saline was perfused from t...

Embodiment 2

[0048] Example 2 In vivo experiment of multiple types of mixed cells on mice with myocardial infarction

[0049] The multi-type mixed cell suspension obtained in Example 1 was injected into the myocardium of mice in the experimental group at 7 days and 28 days after myocardial infarction.

[0050] Establishment of the myocardial infarction model in mice: C57 mice, male or female, weighing 30 g, were anesthetized by intraperitoneal injection of 1% pentobarbital at a concentration of 0.1 mL / 10 g. The supine position was fixed, the skin of the incision was disinfected with alcohol, and a 1.5 cm incision was made at the third intercostal space in the heart. Tie a purse knot with 4-0proline silk thread and set aside. Bluntly dissect the muscle with microscopic forceps. Pierce the intercostal muscles, squeeze out the heart with the left hand, and peel off the pericardium. With the lower edge of the left atrial appendage as a mark, the left coronary artery was ligated with 6-0prol...

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Abstract

The invention discloses an application of multi-type mixed cells in myocardial infarction cell treatment, wherein the multi-type mixed cells are cells derived from vein bridge vessels after a vein istransplanted to an artery. The invention also provides the application of the multi-type mixed cells as a medicament for preventing or treating myocardial infarction. When the multi-type mixed cell disclosed by the invention is applied to myocardial infarction treatment, the cardiac function after myocardial infarction can be improved, the left ventricular ejection fraction can be increased, the infarct area and the wall thickness can be reduced, and the inner diameter of the ventricle can be reduced, so that symptoms can be improved, and the application has important clinical significance.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of multi-type mixed cells in the treatment of myocardial infarction cells. Background technique [0002] Myocardial infarction (MI) is an acute and persistent coronary artery disease characterized by severe and persistent substernal pain that cannot be completely relieved by rest and drugs, accompanied by increased serum myocardial enzyme spectrum activity and progressive ECG changes. Myocardial necrosis caused by ischemia and hypoxia. In recent years, research and development personnel have conducted a number of clinical studies. The results of the research show that the anatomical stenosis of single or multiple coronary artery atherosclerosis is the disease basis of MI. Factors lead to coronary atherosclerotic plaque rupture, platelet aggregation in the coronary lumen, activation of prothrombin to form thrombus, resulting in myocardial necrosis. In additio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/44A61P9/10
CPCA61K35/44A61P9/10
Inventor 葛均波石洪涛孙爱军骆晨涛孙晓垒
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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