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Integrated fluorescent test paper for detecting mercaptoacetic acid in real time and application

A technology of thioglycolic acid and test paper, which is applied to the integrated fluorescent test paper for real-time detection of thioglycolic acid and its application field, can solve the problems of inability to obtain detection results in time, long sample processing and detection time, etc. The effect of simple method

Active Publication Date: 2020-05-15
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the analytical methods now, such as liquid chromatography-mass spectrometry, chromatography, etc., require large-scale instruments, professional technicians and a long time for sample pretreatment and detection, and the detection results cannot be obtained in time

Method used

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  • Integrated fluorescent test paper for detecting mercaptoacetic acid in real time and application
  • Integrated fluorescent test paper for detecting mercaptoacetic acid in real time and application
  • Integrated fluorescent test paper for detecting mercaptoacetic acid in real time and application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The preparation of copper nanoclusters with glutathione as a stabilizer is carried out according to the following steps at room temperature:

[0034] (1) Preparation of 0.1 M copper chloride solution: weigh 1.7048 g CuCl 2 ∙2H 2 O was dissolved in 100 mL of high-purity water, fully dissolved for later use;

[0035] (2) Preparation of copper nanoclusters: at room temperature, 0.29 g glutathione was weighed and dissolved in 15 mL H 2 O, to which was added 400 mL of CuCl 2 (0.1M), after fully reacting, add 0.1 g ascorbic acid (AA), then add 1.2 mL NaOH (1M), react for 1 h, until the white suspension is completely dissolved and turns into a light yellow clear solution, which proves that the copper nanoclusters form.

Embodiment 2

[0037] Copper nano-clusters are used as a method for fluorescent probe specific detection of thioglycolic acid, which is characterized in that it is carried out according to the following steps:

[0038] (1) First cut the filter paper into strips.

[0039] (2) Prepare a copper nanocluster solution synthesized with glutathione as a protective agent (see Example 1).

[0040] (3) Soak the cut filter paper strips in the copper nanocluster solution (3.8 mM, 5 mL), soak for 20 minutes, and dry at room temperature for about 30 minutes.

[0041] (4) After drying at room temperature, place the filter paper strip under ultraviolet light (under the condition of 365 nm), and you will immediately find that the filter paper strip has significant fluorescence.

[0042] (5) Drop 5 drops of TGA solution (100 μM) on a section of the prepared luminescent test paper, and after the test paper is dry (30 minutes), put the test paper under the ultraviolet lamp (under the condition of 365 nm), it is...

Embodiment 3

[0044] Copper nano-clusters are used as a method for fluorescent probe specific detection of thioglycolic acid, which is characterized in that it is carried out according to the following steps:

[0045] (1) First cut the filter paper into strips.

[0046] (2) Prepare a copper nanocluster solution synthesized with glutathione as a protective agent (see Example 1).

[0047] (3) Soak the cut filter paper strips in the copper nanocluster solution (3.8 mM, 5 mL) for 30 minutes, and then dry them at room temperature for about 60 minutes.

[0048] (4) After drying at room temperature, place the filter paper strip under ultraviolet light (under the condition of 365 nm), and you will immediately find that the filter paper strip has significant fluorescence.

[0049] (5) Drop 5 drops of TGA solution (100 μM) on a section of the prepared luminescent test paper, and after the test paper is dried (60 minutes), put the test paper under the ultraviolet lamp (under the condition of 365 nm), i...

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Abstract

The invention discloses integrated fluorescent test paper for detecting mercaptoacetic acid in real time and application. A filter paper strip is soaked in a 3.8 mM copper nano-cluster solution. The test paper can be used as fluorescent test paper for real-time, rapid and visual detection of sulfur-containing pollutant thioglycolic acid (TGA) in the environment. The test paper is prepared by uniformly infiltrating a filter paper strip with a certain shape into a copper nano-cluster solution synthesized by taking glutathione as a protective agent, drying and shaping. The copper nano-cluster fluorescent test paper has excellent fluorescent performance, fluorescence of the copper nano-cluster fluorescent test paper can be effectively quenched by mercaptoacetic acid, real-time rapid analysis and detection of mercaptoacetic acid in an actual environment sample can be realized, and the fluorescent test paper has extremely high application value and market prospect.

Description

[0001] This patent has been supported by the National Natural Science Foundation of China General Project (No.21375095), Tianjin Natural Science Foundation Youth Project (No.17JCQNJC05800), National Environmental Protection Odor Pollution Control Key Laboratory Open Fund Project (No.201903201) and Tianjin Normal University Supported by the university's "Future Thousand Talents Program" project (WLQR201913). technical field [0002] The invention belongs to the application field of metal copper nano-clusters in real-time detection, and specifically relates to a luminescent test paper prepared based on the fluorescence characteristics of copper nano-clusters synthesized with glutathione as a protective agent, and further real-time detection of thioglycolic acid. Background technique [0003] Thiols (RSH) play an important role in the biogeochemical cycling of sulfur, cellular redox and detoxification of cations, and the chemical speciation of many trace metals. In the environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 张菲张俊华李妍孟洁张子彤霍建中区杨靖方照临
Owner TIANJIN NORMAL UNIVERSITY
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