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An endophytic fungus that can enhance casuarina acid phosphatase activity under low phosphorus environment

A technology of endophytic fungi and casuarina, applied in the field of microorganisms to achieve the effect of enhancing acid phosphatase activity and promoting plant growth

Active Publication Date: 2022-02-01
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Endophytic fungi can enhance plant physiological characteristics under adversity stress, but there are few reports on endophytic fungi enhancing the stress resistance of woody host plants

Method used

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  • An endophytic fungus that can enhance casuarina acid phosphatase activity under low phosphorus environment
  • An endophytic fungus that can enhance casuarina acid phosphatase activity under low phosphorus environment
  • An endophytic fungus that can enhance casuarina acid phosphatase activity under low phosphorus environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Isolation of endophytic fungi from casuarina

[0013] 1. Main equipment

[0014] Ultra-clean workbench SW-CJ-1FD, constant temperature incubator HH•B11-II, constant temperature culture oscillator zhwy-211B, ten-thousandth balance AR1140, automatic vertical sterilizer LMQ.C-4060, ultrapure water Machine P60-CW etc.

[0015] 2. Main Reagents and Media

[0016] Reagents: 15% sodium hypochlorite, 75% ethanol, primer PAGE 11-59bp OD 1-2, DNA electrophoresis loading buffer, GoodViewTM nucleic acid dye, 2×Tap PCR MasterMix, fungal DNA extraction kit, DNA purification and recovery kit.

[0017] Medium: (1) Modified Martin agar medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o 6 •H 2 O) 20.0g, Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0 g, magnesium sulfate (MgSO 4 •7H 2 O) 0.5g, agar 15.0g, ultrapure water 1000ml, pH 6.2~6.6.

[0018] (2) Improved Martin's liquid medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o ...

Embodiment 2

[0039] Bacterial solution preparation: The screened endophytic fungi (G3) were cultured in potato dextrose agar medium at 28°C for one week, then inserted into 60 mL potato dextrose liquid medium, and cultured in a constant temperature shaker for 72 h (28°C, 160 r min -1 ), calculate the number of spores by hemocytometer, and dilute the cultured bacterial solution to 5.5×10 by ten-fold dilution method with sterile water 6 pcs L -1 . After that, it was diluted with 40 mL of sterile water, and this concentration and dosage were used for inoculation in subsequent experiments.

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Abstract

The invention discloses an endophytic fungus capable of improving the activity of casuarina acid phosphatase in a low-phosphorus environment. The endophytic fungus Herpotrichia australis G3 has been registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on November 20, 2019, with the preservation number CGMCC No.18816. The strain is isolated and purified from the root of casuarina, which can significantly increase the acid phosphatase activity of casuarina, thereby enhancing the adaptability of seedlings in a low-phosphorus environment.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an endophytic fungus capable of enhancing the activity of casuarina acid phosphatase in a low-phosphorus environment. Background technique [0002] Acid phosphatase is an induced enzyme and hydrolase widely distributed in different tissues of plants. It releases inorganic phosphorus by degrading organic phosphorus compounds, and promotes the absorption, activation and reuse of phosphorus in plants. Synthesis of phosphatase inside and outside plant cells in phosphorus-deficient environment. The phosphatase secreted outside the cell will promote the activation of organic acids and improve the availability of phosphorus in the soil. The acid phosphatase secreted by plant roots can hydrolyze phospholipids in the soil, making the soil insoluble Phosphorus is activated for uptake and utilization by plants. Therefore, the acid phosphatase synthesized and secreted by plant leav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
CPCA01G7/06A01C11/02
Inventor 梁安洁李键吴承祯洪滔林勇明
Owner FUJIAN AGRI & FORESTRY UNIV
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