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Application of abscisic acid in promoting spore production of arbuscular mycorrhizal fungi

A technology for arbuscular mycorrhizal fungi and abscisic acid, which is applied in the application field of abscisic acid in promoting spore production of arbuscular mycorrhizal fungi, can solve the problems of insufficiency, the spore production of arbuscular mycorrhizal fungi needs to be improved, etc. Simple operation, increased spore production, and the effect of increasing production

Inactive Publication Date: 2020-04-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is carried out under non-sterile conditions, and the obtained spores of arbuscular mycorrhizal fungi carry miscellaneous bacteria, which cannot meet the requirements of many scientific researches. In addition, the spore production of arbuscular mycorrhizal fungi still needs to be improved

Method used

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  • Application of abscisic acid in promoting spore production of arbuscular mycorrhizal fungi
  • Application of abscisic acid in promoting spore production of arbuscular mycorrhizal fungi

Examples

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Effect test

Embodiment 1

[0030] Example 1 The impact of abscisic acid on the spore production of arbuscular mycorrhizal fungi under non-symbiotic conditions

[0031] In this example, the model strain of arbuscular mycorrhizal fungi (Rhizocystis heteromorpha DAOM197198) is selected to study the impact of abscisic acid on the spore production of arbuscular mycorrhizal fungi under non-symbiotic conditions. The specific experimental methods and experimental results are respectively as follows:

[0032] 1. Experimental method

[0033] The manufacturing method of the two-compartment petri dish in this embodiment is as follows: a 1.2cm×8.3cm aluminum plate is glued to the middle of a 9cm petri dish with glass glue to make a uniform two-compartment petri dish, and the two compartments are respectively the root compartment (RC) and spore chamber (SC), the root chamber is used to cultivate tomato hairy roots, and the spore chamber is used to inoculate and cultivate spores of arbuscular mycorrhizal fungi. Afte...

Embodiment 2

[0048] The impact of embodiment 2 abscisic acid on the production of arbuscular mycorrhizal fungus spores under symbiotic conditions

[0049] 1. Experimental method

[0050] The culture dish used in this example is an ordinary glass culture dish, and the formula of the MSR medium used is the same as that of the MSR medium in Example 1.

[0051] (1) Configure the MSR medium according to the above formula, add 10g / L of sucrose, adjust the pH of the solution to 5.5, and distribute it in conical flasks; add 2.5g / L of plant gel in each conical flask, After sealing the mouth, sterilize at 121°C for 15 minutes under high temperature and high pressure;

[0052] 2) Weigh 0.0132g of abscisic acid powder, put it in a 10mL centrifuge tube, dissolve it with 100μL of 1M NaOH, dilute to 5mL, and configure 10 -2 The abscisic acid solution of M was sterilized by filtration with a 0.22 μm inorganic phase polyethersulfone (PES) filter membrane, and the abscisic acid solution was diluted to 10 ...

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Abstract

The invention discloses an application of abscisic acid in promoting spore production of arbuscular mycorrhizal fungi. According to the invention, abscisic acid is added into spores of the arbuscularmycorrhizal fungi, the abscisic acid can directly act on the spores of the arbuscular mycorrhizal fungi, the spore yield of the arbuscular mycorrhizal fungi is remarkably improved, and the spore yieldof the arbuscular mycorrhizal fungi under a symbiotic condition can also be remarkably improved by adding the abscisic acid. The abscisic acid can be widely used for promoting spore production of thearbuscular mycorrhizal fungi. The invention further provides a preparation and a method for promoting spore production of the arbuscular mycorrhizal fungi. According to the method, the arbuscular mycorrhizal fungi can be propagated under an aseptic condition, the propagation efficiency is high, the sporulation quantity of the arbuscular mycorrhizal fungi can be remarkably increased, and the method is easy and convenient to operate, low in cost and short in period and has wide application and popularization prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to the use of abscisic acid in promoting sporulation of arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) is a biotrophic, soil-dwelling symbiotic fungus belonging to the Glomeromycotina subphylum, which can establish a symbiotic relationship with the roots of most land plants. After establishing a symbiotic relationship between arbuscular mycorrhizal fungi and plants, it can greatly promote the absorption of mineral nutrients by the roots of crops, such as phosphorus and nitrogen, and improve the stress resistance of crops, such as improving the crops' resistance to nutrient deficiency, drought, heavy metal poisoning, pH stress, Tolerance of diseases and insect pests, etc., improves the growth potential of crops, thereby increasing the yield of crops and improving the quality of crops. Therefore, arbuscular mycorrhizal fung...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/38C12R1/645
CPCC12N1/38C12N3/00
Inventor 姚青刘晓迪冯曾威朱红惠
Owner SOUTH CHINA AGRI UNIV
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