Construction method of hyperglycemic animal model

Abstract

The present invention discloses a construction method of a hyperglycemic animal model. SPF grade C57BL / 6 mice are selected, the mice are intraperitoneally injected with (38-42) mg / kg BW streptozotocinsolution for 5 consecutive days, and fasting for 3-5 h is conducted before injection; fasting for 18-22 h is conducted after 3 days and then intraperitoneal injection of (95-105) mg / kg BW streptozotocin solution is conducted once; and on the 3rd day after all injections are completed, fasting blood glucose value is measured and the mice with the blood glucose value greater than 12 mmol / L are typeI hyperglycemic animals. The suitable streptozotocin solution injection method is designed, administrations in multiple times are conducted, different sizes of injection dose, time interval and fasting time are used, high-fat and high-sugar feed and STZ combined induction is not needed, animals are high in survival rate, modeling period is short, modeling success rate is high, and the animal model has a stable blood glucose value and relatively low cost, and can be used as a research model for hypoglycemic drugs and pathogenesis of diabetes.

Description

technical field [0001] The invention relates to the field of biological model construction, in particular to a method for constructing a hyperglycemia animal model. Background technique [0002] The high incidence of diabetes and many complications have brought a heavy burden to human health and social development. The current treatment methods mainly use drugs or insulin injections to increase the secretion of insulin to lower blood sugar. The side effects of drugs are relatively high and the cost is high. Therefore, research on the treatment of diabetes has become a key research content for scientists. Among them, the animal model is an indispensable and important means and method for the study of diabetes, which will help to further reveal the pathogenesis and intervention measures of diabetes, and test and screen various hypoglycemic drugs. [0003] Studying the treatment of diabetes requires better animal models. At present, most diabetes models are genetically enginee...

AI Technical Summary

Problems solved by technology

The preparation of diabetic animal models is related to the way and dose of STZ injection. Large-dose injection can directly cause extensive destruction of pancreatic β-cells, resulting in a diabetic model, but there are problems of high mortality and poor molding rate; low-dose STZ can only It can destroy the function of a part of pancreatic islet β cells, causing the peripheral tissue to be insensitive to insulin. It needs to be fed with high-calorie feed at the same time. The method of this drug combined with high-fat induction has a long experimental period

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