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Creatine kinase isozyme assay kit

A creatine kinase and kit technology, which is applied to the determination/inspection of microorganisms, measuring devices, instruments, etc., can solve the problems of affecting the precision of detection and prolong the service life of reagents, and achieve high accuracy, high sensitivity and high accuracy. and the effect of increased precision

Pending Publication Date: 2020-04-24
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention can be used for automatic biochemical analyzer detection, easy to operate, can still ensure the accuracy of reagent determination after long-term storage of CK and its isozyme determination reagents, and can significantly prolong the service life of reagents, but due to the existence of ionic liquids There are many kinds of ions in the detection kit, so it will affect the precision of detection to a certain extent

Method used

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  • Creatine kinase isozyme assay kit

Examples

Experimental program
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Embodiment 1

[0064] The components and concentrations of the creatine kinase isoenzyme assay kit of the present embodiment are as follows:

[0065]

[0066]

Embodiment 2

[0068] The components and concentrations of the creatine kinase isoenzyme assay kit of the present embodiment are as follows:

[0069]

Embodiment 3

[0071] The components and concentrations of the creatine kinase isoenzyme assay kit of the present embodiment are as follows:

[0072]

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Abstract

The invention discloses a creatine kinase isozyme assay kit, belonging to the technical field of biological detection. The kit comprises a reagent R1 and a reagent R2 which are independent of each other. The reagent R1 comprises the following components: imidazole acetate, D-glucose, magnesium acetate L, hexokinase, N-acetylcysteine, adenosine diphosphate (ADP), an oxidized coenzyme II (NADP+), sodium azide, ethylenediamine tetraacetic acid (EDTA), glucose-6-phosphate dehydrogenase, adenosine 5'-disodium monophosphate (AMP), and diadenosine pentaphosphate (P1P5-diAP). The reagent R2 comprisesthe following components: creatine phosphate, sodium azide, and an anti-human CK-M antibody. In the implementation process, the stability, accuracy and precision of the kit are obviously improved by controlling the concentration ratio of all the components within a range disclosed by the invention, and the kit is simple to operate, low in preparation cost and suitable for promotion and application.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a creatine kinase isozyme assay kit. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) is a key enzyme in cell energy metabolism, and its activity measurement is the diagnostic basis for clinical skeletal muscle diseases and myocardial diseases. CK is composed of M and B subunits. There are three subunits (isoenzyme) combinations CK-MM, CK-MB, and CK-BB. The muscle type (MM) mainly exists in various muscle cells, and the brain type (BB) It mainly exists in brain cells, and the hybrid type (MB) mainly exists in cardiomyocytes. Creatine kinase MB isoenzyme (CK-MB) is the most specific enzyme in the myocardial enzyme spectrum. [0003] Creatine kinase isoenzymes are of great significance in clinical diagnosis. When various lesions including muscle atrophy and myocardial infarction occur, the level of creatine kinase in human serum increases rapidly. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/48C12Q1/32G01N33/573
CPCC12Q1/485C12Q1/32G01N33/573G01N2333/9123G01N2333/91215G01N2333/904
Inventor 商忆文林耀文
Owner 浙江夸克生物科技有限公司
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