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Method for building triple negative breast cancer living tumor tissue mouse orthotopic transplantation model

A triple-negative breast cancer and tumor tissue technology, applied in animal husbandry and other directions, can solve problems such as poor effect, and achieve the effect of increasing tumor formation rate, increasing estrogen level, and early time.

Pending Publication Date: 2020-04-21
南京普恩瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after long-term in vitro culture of human tumor cell system, its oncological biological behavior, gene expression level and tumor heterogeneity are quite different from those of tumor tissue, which is not good for predicting the results of clinical drug efficacy experiments.

Method used

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  • Method for building triple negative breast cancer living tumor tissue mouse orthotopic transplantation model

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Embodiment 1

[0021] The method for establishing the mouse orthotopic transplantation model of living tumor tissue of triple-negative breast cancer, the steps include: (1) subcutaneously implant 1 piece of estradiol sustained-release tablet in the neck of NCG female mouse after skin surface disinfection, the estradiol sustained-release tablet The dose of diol sustained-release tablet is 0.36mg / tablet, and the sustained-release time is 60 days; (2) on the day of transplantation, the living tumor tissue of triple-negative breast cancer of a patient with clinical triple-negative breast cancer was selected through surgery, and then soaked in green In the preservation solution of streptomycin double antibody, transport at 4°C at low temperature, wash 3 times with sterile preservation solution after delivery to the laboratory, and then bluntly separate the capsule of triple-negative breast cancer living tumor tissue under a microscope, and remove it. For adipose tissue and necrotic tissue, cut the...

Embodiment 2

[0023] The method for establishing the mouse orthotopic transplantation model of living tumor tissue of triple-negative breast cancer, the steps include: (1) subcutaneously implant 1 piece of estradiol sustained-release tablet in the neck of NCG female mouse after skin surface disinfection, the estradiol sustained-release tablet The dose of diol sustained-release tablet is 0.36mg / tablet, and the sustained-release time is 60 days; (2) on the day of transplantation, the living tumor tissue of triple-negative breast cancer of a patient with clinical triple-negative breast cancer was selected through surgery, and then soaked in green In the preservation solution of streptomycin double antibody, transport at 4°C at low temperature, wash 3 times with sterile preservation solution after delivery to the laboratory, and then bluntly separate the capsule of triple-negative breast cancer living tumor tissue under a microscope, and remove it. For adipose tissue and necrotic tissue, cut the...

Embodiment 3

[0025] The method for establishing the mouse orthotopic transplantation model of living tumor tissue of triple-negative breast cancer, the steps include: (1) subcutaneously implant 1 piece of estradiol sustained-release tablet in the neck of NCG female mouse after skin surface disinfection, the estradiol sustained-release tablet The dose of diol sustained-release tablet is 0.36mg / tablet, and the sustained-release time is 60 days; (2) on the day of transplantation, the living tumor tissue of triple-negative breast cancer of a patient with clinical triple-negative breast cancer was selected through surgery, and then soaked in green In the preservation solution of streptomycin double antibody, transport at 4°C at low temperature, wash 3 times with sterile preservation solution after delivery to the laboratory, and then bluntly separate the capsule of triple-negative breast cancer living tumor tissue under a microscope, and remove it. For adipose tissue and necrotic tissue, cut the...

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Abstract

The invention discloses a method for building a triple negative breast cancer living tumor tissue mouse orthotopic transplantation model. The method comprises the following steps of (1) subcutaneouslyembedding an estradiol sustained-release tablet in the neck of an immunodeficient mouse; (2) selecting triple negative breast cancer living tumor tissues of a clinical triple negative breast cancer patient through an operation, and then cutting the triple negative breast cancer living tumor tissues into inoculation tumor blocks with the volume being 4 to 15 mm<3>; (3) performing orthotopic transplantation on the inoculation tumor blocks into a mammary gland fat pad of the immunodeficient mouse; and (4) raising the immunodeficient mouse for 30 to 80 days, and building the triple negative breast cancer living tumor tissue mouse orthotopic transplantation model. The method has the advantages that the estrogen level in a female mouse model body can be improved; and the growth requirement of the triple negative breast cancer tissues is met, so that the tumor nodule emergence time is early, the model building time is shortened, the tumor forming rate is improved by nearly 4 times, and the model building method is simple and feasible.

Description

technical field [0001] The invention relates to a method for establishing a tumor tissue orthotopic model, in particular to a method for establishing a mouse orthotopic transplantation model of triple negative breast cancer living tumor tissue. Background technique [0002] Clinically, breast cancer has become the first female malignant tumor, and its treatment methods and research are also constantly improving. Among them, triple-negative breast cancer is a special subtype of breast cancer, accounting for about 10.0% to 20.8% of the pathological types of breast cancer. The prognosis of this disease is poor and the risk of death is high. treatment guidelines. [0003] Because triple-negative breast cancer lacks expression of ER, PR, and HER2, endocrine therapy and HER2-targeted therapy do not work for this cancer. Although triple-negative breast cancer is relatively sensitive to chemotherapy, only about 20% of patients usually have a good chemotherapy effect through conven...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2267/03A01K2227/105
Inventor 朱燕萍王志强郝艳鹏李丹丹朱宁颖
Owner 南京普恩瑞生物科技有限公司
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