Method for constructing liquid chromatography fingerprint spectrum of abrus cantoniensis hance amide components
A chicken bone oxalamide and liquid chromatography technology, applied in the field of drug quality control, can solve problems such as difficult separation and analysis, poor resolution, and provide technical support and evidence, achieving good precision and reproducibility, peaks Uniform distribution and high separation effect
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[0030] (1) Instruments and medicines: Agilent 1290 ultra-high performance liquid chromatography was used. Twenty-four batches of Jigucao herbs from different origins and harvesting periods were identified as the dried leaves of the leguminous plant Acacia genus Abrus cantoniensis Hance or Abrus mollis Hance. N-trans-p-coumaroyl tyrosine, gallinacein A, gallinacein B and gallinacein C reference substances (made by the New Drug Research and Development Center of Guangdong Pharmaceutical University). Methanol and acetonitrile are chromatographically pure, other reagents are analytically pure, and water is pure water. The concentration percentages of the reagents in this test are volume percentages.
[0031](2) Preparation of the test solution: Weigh 2 g of the herbal material Jigucao, weigh it accurately, place it in a conical flask, add 60 mL of 50% ethanol solution, heat and reflux 3 times, each time for 1 hour, filter, and combine the filtrates , concentrated under reduced p...
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