A method for constructing liquid chromatography fingerprints of chicken bone oxalamide components

Abstract

The invention belongs to the technical field of drug quality control, and in particular relates to a method for constructing a liquid chromatography fingerprint of chicken bone oxamide components. In the present invention, the fingerprints of the chicken bone oxamide components are obtained by means of a high-performance liquid chromatograph. The method has the characteristics of stable identification, good precision and reproducibility, high resolution, and easy operation. The obtained fingerprints of chicken bone oxamide components have even distribution of peaks, and the advantages of common peak characteristics are more obvious. It can be obtained from the chromatogram It can be used for the identification, quality analysis and evaluation of chicken bone oxamide components, as well as related preparations of chicken bone herbal medicines and amide-containing extracts. , Product identification, quality evaluation and control.

Description

technical field [0001] The invention belongs to the technical field of drug quality control, and in particular relates to a method for constructing a liquid chromatography fingerprint of chicken bone oxamide components. Background technique [0002] With the realization of the modernization of traditional Chinese medicine, it has gradually become a consensus that a complete quality control method of traditional Chinese medicine should be established, and many countries have accepted the quality control method of chromatographic fingerprint. The fingerprint of traditional Chinese medicine is a spectrum that characterizes and describes the chemical information of a certain type of chemical substance or each chemical substance in the chemical composition of traditional Chinese medicine in a graphical (image) manner from both qualitative and quantitative perspectives with the help of modern instrumental analysis methods. It can more comprehensively reflect the relative relations...

AI Technical Summary

Problems solved by technology

Previous studies have found that Abrusamide is rich in amide components, such as Abrusamide A, Abrusamide B, Abrusamide B, and N-trans-p-coumaroyltyramide acid, etc., the amide component has good anti-oxidation, anti-inflammatory, liver protection and other activities, and has obtained patent protection (ZL201210421480.X), and established an extraction and preparation method for this part (invention patent application 201610839080.9), but its quality evaluation The method has not obtained good separation conditions, especially the components of this type are isomers and conformational isomerism, which has always been a difficulty in separation and analysis

[0003] Moreover, the quality control of Jigucao under the 2015 edition of the "Chinese Pharmacopoeia" is based on trait identification and thin-layer chromatography of reference medicinal materials. The methods of quality evaluation and quality identification are relatively monotonous, and only rely on trait identification and TLC It is difficult to better evaluate Jigucao by chromatographic analysis, especially for other types of components and effective parts except abrin. There is no identification and determination method for active parts, and it is difficult to determine the quality of Jigucao medicinal materials and their preparations. Provide technical support and basis for control and evaluation

[0004] Therefore, for amides components, in order to better carry out problems such as its quality evaluation and application, the present invention establishes a method for constructing liquid chromatography fingerprints of chicken bone oxalamides components. The method has high resolution, high It has the characteristics of high precision, strong operability, good stability and reproducibility, etc., and overcomes the defect of poor separation under common analysis conditions. Identification, quality evaluation and control of related preparation products

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