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Preparation method of earthworm extract with skin aging resistance

An anti-aging skin extract technology, applied in the field of biochemistry, can solve the problems of heavy fishy smell of the extract and affect product development, etc., and achieve the effect of good sterilization effect, good application value and mild conditions

Inactive Publication Date: 2020-04-17
江西沐恩堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing preparation technology, on the one hand, these active ingredients and other small molecular substances such as vitamins and trace elements contained in the earthworm body are often ignored; on the other hand, the preparation of the extract has a strong fishy smell, which affects the subsequent development of the product

Method used

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  • Preparation method of earthworm extract with skin aging resistance
  • Preparation method of earthworm extract with skin aging resistance

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A preparation method of earthworm extract with anti-skin aging effect, comprising the following steps:

[0025] (1) Soak the fresh earthworms in pure water until there is no sediment, wash the earthworms, and soak them in salt water with a concentration of 0.02% by mass for 45 minutes, then remove the earthworms, and continue After soaking for 30 minutes, remove and drain; wherein the sterilizing solution is composed of Houttuynia cordata extract, wormwood extract, and allicin in a mass ratio of 3:2:1;

[0026] (2) After mashing the fresh earthworms treated in step (1), add water to adjust the solution to a content of 35%, adjust the pH of the solution to 6.5, stir and react at 30°C for 120min, then place it at a constant temperature at 36°C for 20min, and filter Obtain enzymatic hydrolyzate and filter residue;

[0027] (3) get the filter residue that step (2) obtains and add water to be adjusted into the solution that content is 35%, add the protease of 0.02 times of ...

Embodiment 2

[0030] A preparation method of earthworm extract with anti-skin aging effect, comprising the following steps:

[0031] (1) Soak the fresh earthworms in pure water until there is no sediment, wash the earthworms, soak them in salt water with a concentration of 0.025% by mass for 35 minutes, then remove the earthworms, and continue After soaking for 25 minutes, remove and drain; wherein the sterilizing solution is composed of Houttuynia cordata extract, mugwort extract, and allicin in a mass ratio of 3:1:1;

[0032] (2) After crushing the fresh earthworms treated in step (1), add water to make a solution with a content of 40%, adjust the pH of the solution to 6.7, stir and react at 35°C for 100min, then place it at a constant temperature at 36°C for 30min, and filter Obtain enzymatic hydrolyzate and filter residue;

[0033] (3) get the filter residue that step (2) obtains and add water to be adjusted into the solution that content is 40%, add the protease of 0.03 times of filte...

Embodiment 3

[0036] A preparation method of earthworm extract with anti-skin aging effect, comprising the following steps:

[0037] (1) Soak the fresh earthworms in pure water until there is no sediment, wash the earthworms, soak them in 0.03% salt water for 25 minutes, then remove the earthworms, and continue After soaking for 20 minutes, remove and drain; wherein the sterilizing solution is composed of Houttuynia cordata extract, wormwood extract, and allicin in a mass ratio of 2:2:1;

[0038] (2) After mashing the fresh earthworms treated in step (1), add water to make a solution with a content of 42%, adjust the pH of the solution to 6.8, stir and react at 38°C for 80min, then place it at a constant temperature at 36°C for 25min, and filter Obtain enzymatic hydrolyzate and filter residue;

[0039] (3) get the filter residue that step (2) obtains and add water to be adjusted into the solution that content is 38%, add the protease of 0.04 times of filter residue weight enzymolysis 45min...

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Abstract

The invention discloses a preparation method of earthworm extract with skin aging resistance. The method comprises the following steps: (1), soaking fresh earthworms in water until no silt exists, soaking the processed earthworms in saline water, continuing to soak the earthworms in a sterilizing solution, performing fishing out, and performing draining; (2), mashing the earthworms, adding water to prepare a solution, performing stirring reaction, then performing constant-temperature placement for 20-40min, and performing filtration to obtain enzymatic hydrolysate and filter residues; (3), adding water into the filter residues to prepare a solution, adding protease to perform enzymolysis, and performing centrifugal filtration to obtain filtrate; and (4), mixing the enzymatic hydrolysate obtained in the step (1) and the filtrate obtained in the step (2) to obtain an extracting solution, carrying out decoloration and deodorization treatment, and then filtering by using an ultrafiltrationmembrane to obtain an earthworm extract. The method is simple in process, mild in condition and free of pollution; the obtained earthworm extract has the capacity of removing superoxide anion free radicals and hydroxyl free radicals; growth and proliferation of human skin fibroblasts can be effectively promoted; and the earthworm extract can be applied to the fields of health care products and cosmetics and has an important application value.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a preparation method of an earthworm extract with anti-skin aging effect. Background technique [0002] Earthworm, commonly known as earthworm, is a high-quality animal protein resource, which belongs to commonly used traditional Chinese medicine. Antipyretic, anti-cerebral ischemia, anti-asthma, anti-bacterial, anti-oxidation, promotion of wound repair and cell regeneration. In addition, the polypeptide extract obtained by enzymatic hydrolysis of earthworms has physiological activities such as anti-oxidation, enhancing immunity, and antibacterial. [0003] At present, the use of earthworm extracts in the development of anti-aging skin products is still relatively small. In the preparation process of earthworm extract, in addition to mainly producing polypeptides, active ingredients such as amino acids and water-soluble small molecular fatty acids will also be produced. In ...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61Q19/08A61K35/62A61P17/18A23L33/00
CPCA61K8/987A61Q19/08A61K35/62A61P17/18A23L33/00A61K2800/805A23V2002/00A23V2200/318
Inventor 付爱东
Owner 江西沐恩堂生物科技有限公司
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