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Construction method of HPLC specific chromatogram of daphne genkwa standard decoction and component content determination method of daphne genkwa standard decoction

A technology of characteristic map and construction method, which is applied in the construction field of HPLC characteristic map of genkwa standard decoction, can solve the problem of not establishing an effective method for controlling the quality of genkwa standard decoction, not yet discovering genkwa standard decoction, and high detection cost problems, to achieve the effect of ensuring effectiveness and safety, stable methods, and rapid quality control

Pending Publication Date: 2020-04-10
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The current quality control methods for this drug mostly use different chromatographic conditions to measure the content of one or two components, which has the disadvantages of cumbersome operation, long detection time, and high detection cost.
[0005] Daphne genkwa standard decoction is made by extracting, concentrating and drying the decoction pieces of a single Chinese herbal medicine. At present, there is no effective method for controlling the quality of Daphne genkwa standard decoction, and HPLC method has not been found in the prior art to simultaneously determine Daphne genkwa. Literature report on the determination of four chemical components in Daphne genkwa standard decoction by the characteristic map of flower standard decoction combined with one test and multiple evaluation method

Method used

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  • Construction method of HPLC specific chromatogram of daphne genkwa standard decoction and component content determination method of daphne genkwa standard decoction
  • Construction method of HPLC specific chromatogram of daphne genkwa standard decoction and component content determination method of daphne genkwa standard decoction
  • Construction method of HPLC specific chromatogram of daphne genkwa standard decoction and component content determination method of daphne genkwa standard decoction

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Experimental program
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Effect test

Embodiment 1

[0039] Construction method of HPLC characteristic chromatogram of Coriander standard decoction

[0040] 1. The chromatographic conditions for high performance liquid chromatography analysis are: use octadecylsilane-bonded silica gel as the filler, the column temperature is 35 °C, methanol is used as mobile phase A, and 0.1% phosphoric acid aqueous solution is used as mobile phase B. Gradient elution, the flow rate was 1 mL / min, the detection wavelength was 350 nm, and the injection volume was 10 μL.

[0041] 2. The gradient elution conditions are: 0~3min, the volume fraction of mobile phase A changes to 20%~43%, and the volume fraction of mobile phase B changes to 80%~57%; 3~25min, the volume fraction of mobile phase A changes The change is 43%~45%, and the volume fraction of mobile phase B changes to 57%~55%; 25~35min, the volume fraction of mobile phase A changes to 45%~60%, and the volume fraction of mobile phase B changes to 55% ~40%; 35~50min, the volume fraction of mobi...

Embodiment 2

[0102] Method for the determination of the content of the ingredients in the standard decoction of coriander flower

[0103] 1. The chromatographic conditions for high performance liquid chromatography analysis are: use octadecylsilane-bonded silica gel as the filler, the column temperature is 35 °C, methanol is used as mobile phase A, and 0.1% phosphoric acid aqueous solution is used as mobile phase B. Gradient elution, the flow rate was 1 mL / min, the detection wavelength was 350 nm, and the injection volume was 10 μL.

[0104] 2. The gradient elution conditions are: 0~3min, the volume fraction of mobile phase A changes to 20%~43%, and the volume fraction of mobile phase B changes to 80%~57%; 3~25min, the volume fraction of mobile phase A changes The change is 43%~45%, and the volume fraction of mobile phase B changes to 57%~55%; 25~35min, the volume fraction of mobile phase A changes to 45%~60%, and the volume fraction of mobile phase B changes to 55% ~40%; 35~50min, the vo...

Embodiment 3

[0132]Using HPLC characteristic chromatogram to identify the standard decoction of Coriander.

[0133] Coriander standard decoction is made in the laboratory.

[0134] 1. The chromatographic conditions for high performance liquid chromatography analysis are: use octadecylsilane-bonded silica gel as the filler, the column temperature is 35 °C, methanol is used as mobile phase A, and 0.1% phosphoric acid aqueous solution is used as mobile phase B. Gradient elution, the flow rate was 1 mL / min, the detection wavelength was 350 nm, and the injection volume was 10 μL.

[0135] 2. The gradient elution conditions are: 0~3min, the volume fraction of mobile phase A changes to 20%~43%, and the volume fraction of mobile phase B changes to 80%~57%; 3~25min, the volume fraction of mobile phase A changes The change is 43%~45%, and the volume fraction of mobile phase B changes to 57%~55%; 25~35min, the volume fraction of mobile phase A changes to 45%~60%, and the volume fraction of mobile ph...

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Abstract

The invention relates to a construction method of a HPLC specific chromatogram of a daphne genkwa standard decoction and a component content determination method of the daphne genkwa standard decoction, and the component content determination method of the daphne genkwa standard decoction comprises the following steps: (1) respectively sucking a reference substance solution and a to-be-determineddaphne genkwa standard decoction sample solution, injecting the solutions into a high performance liquid chromatograph, and determining corresponding peak areas; (2) calculating the content of genkwanin through an external standard method; and (3) calculating the contents of apigenin-7-O-beta-glucuronide, apigenin and hydroxygenkwanin through a quantitative analysis of multiple components by single marker. According to the method, the content of genkwanin is calculated through an external standard method, and then the content of apigenin-7-O-beta-glucuronide, apigenin and hydroxygenkwanin is calculated through a quantitative analysis method, so that the operation steps of content determination of four components can be effectively simplified, the determination time is shortened, the working efficiency is improved, and quantitative analysis of chemical components of the genkwanin standard decoction is realized.

Description

technical field [0001] The invention belongs to the field of identification of traditional Chinese medicines, and particularly relates to a method for constructing an HPLC characteristic spectrum of a daisy flower standard decoction and a method for determining the content of its components. Background technique [0002] Coriander is the dried flower bud of Corianderaceae. Picked in spring when the flowers are not open, remove impurities, dry, wash and scrape off the rough skin while fresh, wash and dry; this product has the functions of purging water and drinking, detoxifying and killing insects. It is traditionally used to treat edema and accumulation of phlegm. and dyspnea cough embolism. Coriander is also known as Southern Coriander, Coriander Strip, Medicinal Fish Grass, etc. It was first recorded in "Shen Nong's Materia Medica" and listed as the lower grade. Modern medical research shows that the main medicinal components of fenugreek are flavonoids, which have vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/86
CPCG01N30/06G01N30/34G01N30/8679G01N30/8686G01N30/88G01N2030/047G01N2030/884
Inventor 马瑞瑞官永河罗宇琴陈向东魏梅孙冬梅李国卫王碧君程学仁
Owner GUANGDONG YIFANG PHARMA
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