Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea

A technology of Bacillus Velez and Bacillus, applied in the field of microbiology, can solve the problem that no one isolates the strain of Bacillus Velez, and achieve the effects of not being easy to die, reducing the rate of diarrhea, and reducing diarrhea

Active Publication Date: 2020-04-10
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past two years, a few studies have found that Bacillus Velez can also be isolated from the intestines of some specific animals, such as the Chinese patent "Bacillus Velez and Its Application as an Inhibitor of Aquatic Pathogens" with publication number CN108676756B ", the Bacillus Velez is isolated from the intestinal tract of tilapia; the Chinese patent "A Strain of Bacillus Velez and Its Application" with the publication number CN110452848A, the Bacillus Velez is obtained from the feces of sika deer It has been isolated, but it has not been reported to apply Bacillus velaisi to pig breeding, and no one has isolated a strain of Bacillus velaisi suitable for use in pig breeding

Method used

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  • Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea
  • Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea
  • Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Isolation and identification of Bacillus sp. strain LL2018013

[0038] Step 1) Screening of Bacillus strains

[0039] Take the contents of the small intestine, cecum and large intestine of Diqing Tibetan pigs in sterilized saline (w / v=1%), shake fully, bathe in water at 80°C for 10 minutes, and after gradient dilution, transfer to LB solid medium for culture. After culturing at 35°C for 12 hours, a single colony with typical characteristics of Bacillus was picked, purified and cultured to obtain Bacillus.

[0040] Step 2) screening of antagonistic strains

[0041] Use the bacillus obtained in step 1) to prepare the bacteria solution to be tested, use Escherichia coli K88, Salmonella typhimurium and Staphylococcus aureus as indicator bacteria, prepare a suspension with 0.9% saline and adjust the concentration, and add it to the agar In the culture medium, prepare about 10 indicator bacteria 5 CFU / mL agar plate, after cooling, use the gel punching method to ...

Embodiment 2

[0058] Embodiment 2 weaned piglet feeding experiment

[0059] Preparation of A1 fresh bacterial solution: Inoculate the above-mentioned Bacillus veleus strain LL2018013 into LB liquid medium at a volume percentage of 0.1%, and culture at 30-35° C. for 20-24 hours to obtain A1 fresh bacterial solution.

[0060] Feeding experiment: 40 piglets weaned on 7 days of Diqing Tibetan pig were selected and randomly divided into two groups, half male and half female. 8 CFU / L), the control group was fed milk replacer, and the test period was 21 days, including 7 days in the pre-test and 14 days in the main test to observe the morbidity and mortality.

[0061] Diarrhea rate = number of diarrhea episodes / (total number of test piglets × positive test days) × 100%

[0062] Table 3 Diarrhea rate and survival rate of weaned piglets

[0063]

Embodiment 3

[0064] Example 3 Breeding pig intestinal flora abundance test

[0065] 1) Prepare A1 dry bacteria powder:

[0066] Inoculate the Bacillus veleus strain LL2018013 described in Example 1 into LB liquid medium according to 0.1% by volume, cultivate at 35°C for 20h, and then centrifuge at 10000rpm to obtain the thallus; add an appropriate amount of purified water to the above thalline and mix to obtain Mud, mixed with protective agent, then add appropriate amount of purified water, spray dry with embedding material to obtain dry bacterial powder;

[0067] Among them, the protective agent components and proportions are: 15% skimmed milk powder, 5% glycerin, 8% R3 resistant starch, 2% vitamin E, 3% maltodextrin, 3% agar powder, and the rest is potato resistant Starch; the embedding material is potato resistant starch, pectin, sodium hydroxymethylcellulose, and carrageenan, and the embedding material is prepared into a 5% aqueous solution according to the ratio of 1:1:1:1 for later ...

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Abstract

The invention relates to bacillus velezensis and an application thereof in prevention and treatment of porcine diarrhea. The invention provides a bacillus velezensis strain LL2018013. The bacillus velezensis is added into a weaned piglet milk replacer, wherein the addition amount is 107 to 109 CFU / L; and the bacillus velezensis is added into a feed for pigs at growth periods, wherein the additionamount is 106 to 108 CFU / g. The bacillus velezensis provided by the invention can improve the diversity of pig intestinal flora, reduces inflammatory factors through regulation of the flora abundance,promotes nutrient digestion and absorption, improves oxidation resistance of a body, improves immunity, increases the resisting capability to viruses, reduces generation of diarrhea, can inhibit common pig intestinal pathogens like escherichia coli K88, salmonella typhimurium and staphylococcus aureus, reduces the rate of diarrhea, improves the daily gain of pigs, can inhibit molds in a feed, improves the metabolic efficiency of the body to lipids, improves digestion and absorption of the pigs to feed nutritional ingredients, and has the trend of reducing chemical residues in excrement at thesame time.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Bacillus velei and its application in the prevention and treatment of swine diarrhea. Background technique [0002] At present, with the improvement of the level of animal husbandry in my country, the livestock and poultry breeding industry has played an important role in my country's agricultural economic industry. With the development of the industry, antibiotics, hormones and other drugs have brought contradictory status quo. On the one hand, they bring rich production benefits, such as: promoting growth, increasing feed remuneration, and reducing morbidity; on the other hand, they destroy the balance of flora and inhibit Immune response, antibiotic residues, environmental pollution caused by antibiotics in animal excrement, deterioration of soil environment, and a series of reactions. [0003] Pigs are the most widely raised livestock in my country, and pig diar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23K10/18A23K50/60A23K50/30C12R1/07
CPCA23K10/18A23K50/30A23K50/60C12N1/04C12N1/20C12N1/205C12R2001/07
Inventor 刘韶娜赵彦光张斌相德才赵智勇
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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