Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of Pseudomonas dispora strain and its breeding method

A technology of A. bisporus and a strain, which is applied in the field of A. bisporus strains and their breeding, can solve problems such as less research on A. bisporus, and achieves consistent development, stable quality and high quality Good results

Active Publication Date: 2021-06-25
SOUTH CHINA AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some strains of the genus Ouderia minor have been domesticated and cultivated domestically (Li Chuanhua et al., 2011). There are few related studies on German mushroom

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Pseudomonas dispora strain and its breeding method
  • A kind of Pseudomonas dispora strain and its breeding method
  • A kind of Pseudomonas dispora strain and its breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Strain isolation

[0046] Choose mushrooms without umbrellas and stalks without cracks, and discard young mushrooms eaten by ants and fruit fly larvae. After wiping the fruiting bodies of young mushrooms with sterile paper or cotton, use sterile tweezers to pick out the internal hyphae, place them in PDA medium containing streptomycin, and cultivate them at 26°C for 7 days. Colonies with high whiteness, uniform density everywhere, and no bacterial contamination around.

[0047] (2) strain purification

[0048] Pick the aerial hyphae in the target colony from the colonies selected in step (1) and insert them into fresh PDA medium to purify the strains, and transfer the strains after 3 times of purification into inclined test tubes, Cultivate at 24°C to 26°C until the slope is covered with hyphae to obtain pure strains for strain preservation. Mycelial morphology and culture characteristics such as figure 1 shown.

[0049] figure 1 A shows that the mycelium of P...

Embodiment 2

[0057] (1) Fermented cultivation material

[0058] Weigh cottonseed husks, the raw material for cultivation, add water and mix well, fully stir with a mixer during adding water to make it fully absorb water, then add an appropriate amount of lime water and mix well to make the pH value 8.0, build a heap and ferment for 8 days, remove The gossypol in the cottonseed shell increases the water holding capacity of the material and softens the material.

[0059] Preparation of solid medium: 85.2% of fermented cottonseed hulls, 10.2% of wheat bran, 2.6% of glucose, 0.3% of magnesium sulfate, 1.2% of potassium dihydrogen phosphate, and 0.5% of calcium carbonate; the water content is 60% to 75%, and the pH is natural Weighing the solid culture medium according to the ratio of parts by mass, adding water and mixing well, fully stirring with a mixer during adding water to make it fully absorb water, adjusting the water content of the solid culture medium to 68%, and pH 8.5.

[0060] (2)...

Embodiment 3

[0092] The pure mycelia obtained in the step (2) of Example 1 was inoculated onto the medium I (malt extract 5g / L, maltose 10g / L, yeast extract 2.5g / L, ammonium sulfate 0.5g / L, sulfuric acid Magnesium 1.7g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, agar 20g / L, distilled water to 1000mL, pH natural), at 20°C, 22°C, 24°C, 26°C , 28°C and 30°C for 7 days at a constant temperature and protected from light, measure the diameter of the colony, and calculate the daily average growth rate. The result is as Figure 7 As shown, it can be seen that Oddya bisporus can grow in the temperature range of 20°C to 30°C, and the growth rate is the fastest at 26°C, and the optimum growth temperature range is 24°C to 26°C, which is lower than the temperature range of 26°C. The hyphae of O. bisporus grow slowly at 22℃ and higher than 28℃.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a strain of Pseudomonas bisporus and a breeding method thereof. The name of the strain of the present invention is Hymenopellis raphanipes MesAdm 1, the preservation number is GDMCC No: 60886, and it was preserved on October 29, 2019 in Building 59, Compound No. 100, Xianlie Middle Road, Guangzhou City. Culture Collection Center. The Pseudomonas bisporus strain of the present invention has a fast growth rate of 8.2000±0.2830 mm / d, can produce mushrooms without covering soil, has a yield as high as 17-22 flowers per bottle, and has a short growth period of up to 58 days. The sporocarp of the Pseudomonas bisporus strain of the present invention is umbrella-shaped, the length of the stipe is 15-23 cm, the diameter of the stipe is 0.5-1.5 cm, the diameter of the cap is 4.0-12 cm, the cap is uniform and moderate, the quality is good, the quality is stable, and the growth unanimous.

Description

technical field [0001] The invention relates to the technical field of edible fungus breeding, in particular to a Pseudomonas bisporus strain and a breeding method thereof. Background technique [0002] Hymenopellis raphanipes belongs to Basidiomycota, Agaricales, pHysalacriceae, Oudemansiella. In recent years, some strains of the genus Ouderia minor have been domesticated and cultivated domestically (Li Chuanhua et al., 2011). There are few related studies on the mushroom. Contents of the invention [0003] The primary purpose of the present invention is to provide a strain of Pseudomonas bisporus. [0004] Another object of the present invention is to provide a breeding method for the above-mentioned Pseudomonas bisporus strain. [0005] The object of the present invention is achieved through the following technical solutions: a strain of Pseudomonas bisporus, called Hymenopellis raphanipes MesAdm 1, and the preservation number is GDMCC No: 60886, which was released o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02A01H15/00C12R1/645
CPCA01H15/00C12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 莫美华王越袁征超苏楚亮杨昱仪卢意薛玲娜查丽燕张振伟文华枢侯跃辉宋舒晴
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com