A method for in vitro preservation of the germplasm resources of the succulent plant of the genus S.
A technology for in vitro preservation and succulent plants, which is applied in the field of in vitro preservation of the germplasm resources of the succulent plant succulents of the succulent plant, can solve the problems of damage to the succulent plants of the succulent plant and the high cost of storage in liquid nitrogen, and reduce the cost and difficulty of preservation. Convenient transportation and storage, low storage cost
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Embodiment 1
[0026] The blue mirror provided by Shaoxing Cat's Claw Garden was used as the source of germplasm.
[0027] Carry out germplasm in vitro preservation test according to technical route of the present invention, detailed steps are as follows:
[0028] A method for in vitro preservation of germplasm resources of the succulent plant Blue Mirror of the genus Tilapia, characterized in that it comprises the following steps,
[0029] (1) Preparation of culture medium: Put the special tank for in vitro preservation of blue mirror germplasm resources into a sterilizing pot for high temperature and high pressure sterilization. Prepare the induction medium, the medium composition is MS+BA 1.0mg / L+TDZ 0.3mg / L. Prepare the differentiation medium, the medium composition is MS+BA 1.0mg / L+TDZ 0.5mg / L. Prepare the suppression medium, the composition of the suppression medium is 1 / 4MS+BA0.2 mg / L+NAA0.02 mg / L+2.5g / L mannitol. A proliferation medium was prepared, and the composition of the prol...
Embodiment 2
[0034] Example 2 is the same as Example 1 except for the inhibition of MS concentration in the culture medium. Example 2 The concentration of MS in the inhibition medium is 1 / 2 MS.
Embodiment 3
[0042] Example 3 is the same as Example 1 except for the inhibitor concentration in the inhibition medium. Example 3 The inhibitor concentration in the inhibition medium is 1.0 g / L mannitol.
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