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High density culturing method of alexandrium tamarense

A technology of Alexandrium tama, high-density culture, applied in the fields of botanical equipment and methods, algae products, unicellular algae, etc., to achieve the effect of high cell density

Inactive Publication Date: 2006-01-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to provide a method for cultivating Alexandrium tamarensis at a high density, so as to solve people's large demand for Alexandrium tamaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Add 2.2 L of medium to a 3 L airlift photobioreactor. Nitrogen (NaNO 3 ) concentration and phosphorus (NaH 2 PO 4 ) concentration is the same as f / 2 enriched artificial seawater, the initial nitrogen (NaNO 3 ) concentration is 0.0882mmol / L, phosphorus (NaH 2 PO 4 ) concentration is 0.036mmol / L. The culture temperature was 22°C, the light source was provided by white fluorescent lamp, and the light intensity was 60 μmol photon m -2 ·s -1 , the illumination is continuous illumination. Insert the seed solution of Alexandrium tamarense, and the inoculation density is 500cells ml -1 . After inoculation, let it stand for 4 days and then pass in sterile air with an air flow rate of 150ml per minute -1 . On the 6th day after inoculation (the nitrogen concentration at this time is 0.028mmol / L, and the phosphorus concentration is 0.021mmol / L), add 0.80mmol / L NaNO 3 , the cell density can reach 20850cells ml on the 14th day -1 ;The highest density of 46630 cells ml wa...

Embodiment 2

[0048] Add 2.2 liters of culture medium in 3 liters of air-lift photobioreactor, culture temperature, photoperiod, inoculation density are the same as embodiment 1. The light intensity is 100μmol·photon·m -2 ·s -1 , the starting medium is the same as in Example 1. Insert the seed solution of Alexandrium tamarense. After the inoculation, keep the aseptic air culture at an interval of 1 hour, and the ventilation rate is 30ml / min. Change the sterile air flow to 150ml min after 4 days of culture -1 . On the 6th day after inoculation (the nitrogen concentration at this time is 0.031mmol / L, and the phosphorus concentration is 0.023mmol / L), add 0.80mmol / L NaNO 3 , the cell density can reach 22850cells ml on the 14th day -1 ;The highest density of 44530 cells ml was reached on the 18th day -1 .

Embodiment 3

[0050] Add 2.2 liters of culture medium in 3 liters of air-lift photobioreactor, culture temperature, light intensity, light cycle, seeding density are the same as embodiment 1. The starting medium is the same as in Example 1. Insert the seed solution of Alexandrium tamarense. After inoculation, let it stand for culture for 5 days, then pass through sterile air, the air flow rate is 100ml per minute -1 . On the 10th day after inoculation (the nitrogen concentration at this time was 0.0026mmol / L, and the phosphorus concentration was 0.009mmol / L) at the same time, 0.80mmol / L NaNO 3 and 0.072mmol / L NaH 2 PO 4 , the cell density can reach 18860cells ml on the 15th day -1 ; reached the highest density of 55600 cells ml on the 17th day -1 .

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Abstract

The invention relates to tiny algae cultivating field, especially the high density cultivating method of Tama Alexander algae. In the initial stages, static laying and mildness whisking would be adopted to make the carbon source and other alimentation in the culture medium proliferate for a period of time. Taking aerate after the cell density reaching a certain extent, thus, injure of cell would be avoid. The gas could have a certain ratio CO2 to supply carbon source that the algae cell needs for growing. And a certain PH value would be sustained. At the initial stages, suitable N, P consistence would be supplied to make the cell grow fast. And at the late stages, N, P should be added to relief the lack of N, P, and achieve high cell density.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and in particular relates to a high-density cultivation method of Alexander algae. Background technique [0002] Alexandrium tamarense, a marine dinoflagellate that produces neuroparalytic toxin (PSP), is a common red tide algae. Toxic red tides caused by it often occur in Japan, Europe and the northeastern coast of North America. Tamar Alexandrium species or its cysts have been found in Jiaozhou Bay and the South China Sea in my country, and the red tide density has been reached in shrimp ponds in Xiamen. Researchers at home and abroad have conducted a lot of research on the environmental and nutritional factors and toxin production rules that cause the algal red tide, such as literature: [0003] 1. Parker NS, Negri AP, Frampton DMF, Rodolfi L, Tredici MR, Blackburn SI. Growth of thetoxic dinoflagellate Alexandrium minutum (Dinophyceae) using high biomass culturessystems. J. Appl. Phycol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12A01H13/00
Inventor 胡晗华石岩峻丛威康瑞娟蔡昭铃
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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