Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of cultivation method of industrialized vase-cultivated Pseudomonas dispora

A technique for cultivating Pseudomonas bisporus and its cultivation method, which is applied in the field of cultivation of industrial bottle-cultured Pseudomonas bisporus, can solve problems such as inconsistent quality, low biotransformation rate and yield, and high strain contamination rate, and achieve production The effect of low cost, short production cycle and simple cultivation method

Active Publication Date: 2021-09-24
SOUTH CHINA AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the cultivation of Ouderia bisporus is still in the traditional production mode, and the traditional production mode has the problems of high strain contamination rate, inconsistent quality, low biotransformation rate and yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of cultivation method of industrialized vase-cultivated Pseudomonas dispora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Fermentation cultivation material: take the cottonseed husks of the cultivation raw material, add water and mix well, fully stir with a mixer in the process of adding water to make it fully absorb water, then add an appropriate amount of lime water and mix well to make its pH value 8.0, Build piles and ferment for 8 days to remove gossypol in the cottonseed husks, increase the water holding capacity of the material and soften the material.

[0046] Preparation of solid medium: 85.2% of fermented cottonseed hulls, 10.2% of wheat bran, 2.6% of glucose, 0.3% of magnesium sulfate, 1.2% of potassium dihydrogen phosphate, and 0.5% of calcium carbonate; the water content is 60% to 75%, and the pH is natural Weighing the solid culture medium according to the ratio of parts by mass, adding water and mixing well, fully stirring with a mixer during adding water to make it fully absorb water, adjusting the water content of the solid culture medium to 68%, and pH 8.5.

[0047] (...

Embodiment 2

[0077] (1) Fermentation cultivation material: take the cottonseed husks of the cultivation raw material, add water and mix well, fully stir with a mixer in the process of adding water to make it fully absorb water, then add an appropriate amount of lime water and mix well to make its pH value 8.5, Build piles and ferment for 9 days to remove gossypol in the cottonseed husks, increase the water holding capacity of the material, and soften the material.

[0078] Preparation of solid medium: 85.2% of fermented cottonseed hulls, 10.2% of wheat bran, 2.6% of glucose, 0.3% of magnesium sulfate, 1.2% of potassium dihydrogen phosphate, and 0.5% of calcium carbonate; the water content is 60% to 75%, and the pH is natural Weighing the solid culture medium according to the ratio of parts by mass, adding water and mixing well, fully stirring with a mixer during adding water to make it fully absorb water, adjusting the water content of the solid culture medium to 70%, and pH 8.5.

[0079] ...

Embodiment 3

[0109] (1) Fermentation cultivation material: take the cottonseed husks of the cultivation raw material, add water and mix well, fully stir with a mixer in the process of adding water to make it fully absorb water, then add an appropriate amount of lime water and mix well to make its pH value 9.0, Build piles and ferment for 10 days to remove gossypol in the cottonseed hulls, increase the water holding capacity of the material, and soften the material.

[0110] Preparation of solid medium: 85.2% of fermented cottonseed hulls, 10.2% of wheat bran, 2.6% of glucose, 0.3% of magnesium sulfate, 1.2% of potassium dihydrogen phosphate, and 0.5% of calcium carbonate; the water content is 60% to 75%, and the pH is natural Weighing the solid culture medium according to the ratio of parts by mass, adding water and mixing well, fully stirring with a mixer during adding water, making it fully absorb water, adjusting the water content of the solid culture medium to 75%, and pH 9.0.

[0111]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for cultivating Pseudomonas bisporus in industrialized bottles. The cultivation method of the present invention comprises the following steps: Cultivation of cultivars: using bottle planting method, cultivating the inoculated Pseudomonas bisporus in the dark, adjusting the temperature according to the feed-to-food ratio of mycelia to obtain cultivars; kink mycelia: scratch Bacteria, open the bottle and place it upside down on a sterilized wet cloth, culture in the dark under controlled temperature, humidity and carbon dioxide concentration conditions, adjust the temperature and humidity after the mycelium recovers, and continue to grow in the dark until the mycelia of Oddryopsis bisporus kinks form Primordia; Budding reminder: Under the conditions of controlling temperature, humidity and carbon dioxide concentration, cultivate the primordia in the dark until the mushroom buds grow to 0.3-0.7cm; Procreation: Cultivate the mushroom buds under the conditions of controlling temperature, humidity, carbon dioxide concentration and light, so as to To achieve the effect of thinning buds and promoting the accumulation of mushroom body biomass, harvest. The cultivation method of the present invention has a short production cycle, and the produced Ouderia bisporus has good quality, stable quality and consistent development.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for cultivating industrialized bottle-cultivated Ouderia bisporus. Background technique [0002] Hymenopellis raphanipes belongs to a new type of edible fungus, belonging to Basidiomycota, Agaricales, pHysalacriceae, and Oudemansiella. At present, there are relatively few reports and studies on O. bisporus, and even fewer studies on cultivation. At present, the cultivation of Ouderia bisporus is still in the traditional production mode, and the traditional production mode has the problems of high strain contamination rate, inconsistent quality, low biotransformation rate and yield. Therefore, it is necessary to explore a new cultivation mode with the characteristics of high yield, high quality and short growth cycle. Contents of the invention [0003] The primary purpose of the present invention is to overcome the shortcomings and deficiencies of th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 莫美华袁征超苏楚亮杨昱仪卢意薛玲娜陈导道姜莹李文
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com