Phosphate-solubilizing penicillium PSF and applications thereof
A technology of Penicillium phosphate solubilization and Penicillium phosphate, applied in the field of Penicillium phosphate solubilization PSF strains, can solve the problems of rapid degradation of phosphorus-solubilizing ability, high strain elimination rate, lack of available phosphorus, etc. Phosphorus deficiency, the effect of promoting growth
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Embodiment 1
[0031] A kind of Penicillium phospholyticum PSF provided by the invention
[0032] 1. Origin of strains
[0033] The samples come from the salinized soil of the Yellow River Delta;
[0034] Selected from the salinized soil of the Yellow River Delta, weighed 10g of the soil sample and placed it in 90mL of sterile water, and oscillated at a speed of 3000 rpm for 3 minutes to make a soil suspension;
[0035] 2. Isolation of strains
[0036] Dilute the soil suspension by 10-fold gradient, and then pipette 10 -4 、10 -5 、10 -6 Suspend 200 μL of the three gradient soils, spread them on the PVK inorganic phosphorus solid medium respectively, then place the inoculated PVK inorganic phosphorus solid medium upside down in a biochemical incubator, and cultivate for 7 days at 28°C. Fifteen strains of phosphorus-dissolving fungi were screened out, among which the strain number PSF had the strongest phosphorus-dissolving ability, and the phosphorus-dissolving circle formed on PVK solid ...
Embodiment 2
[0049] Solubilization Effect of Penicillium phospholyticum PSF on Various Insoluble Phosphates
[0050] This strain is mainly for four insoluble phosphates namely Ca 5 (PO 4 ) 3 OH, AlPO 4 , Zn 3 (PO 4 ) 2 and FePO 4 Phosphate-dissolving effect;
[0051] Preparation of Penicillium phospholyticum PSF bacterial suspension: according to volume fraction ratio, add 5 parts of pbs buffer solution to 1 part of Penicillium phospholyticum PSF, mix evenly, make Penicillium phospholyticum PSF bacterial suspension, set aside;
[0052] Ca5(PO 4 )3OH、AlPO 4 , Zn 3 (PO 4 ) 2 、FePO 4 The dissolution situation, take the preparation of Ca5(PO4)3OH phosphate detection solution as an example:
[0053] Add 50mL PVK liquid medium, 0.5g Ca 5 (PO 4 ) 3 OH, 500 μL of Penicillium phospholyticum PSF bacterial suspension, cultured on a shaker at a speed of 200 r / min for 5 to 7 days at a temperature of 28 ° C, to obtain Ca 5 (PO 4 ) 3 OH phosphate detection solution;
[0054] Accordin...
Embodiment 3
[0060] Preparation of Fermentation Broth of Penicillium phospholyticum PSF
[0061] Inoculate Penicillium phospholyticum PSF on PDA medium, culture at 28°C for 3 days, inoculate the agar block with a diameter of 7mm containing PSF mycelia by punching method, place it in PVK liquid medium at 28°C, and shake The rotational speed was 180 rpm, and the shaking flask was fermented for 96 hours to obtain the Penicillium phospholyticum PSF fermentation broth, which was set aside.
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