Method for promoting degradation of agricultural waste by thermobifida fusca

A thermophilic fungus and thermal cracking technology, applied in the field of microorganisms, can solve the problems such as the inability to degrade lignocellulose by a single enzyme and increase the cost.

Inactive Publication Date: 2020-03-31
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many heat-resistant enzymes with excellent performance have been cloned, it is impossible for a single enzyme to complete the degradation of lignocellulose, and the heterologous expression of a series of enzymes will also increase the cost

Method used

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  • Method for promoting degradation of agricultural waste by thermobifida fusca
  • Method for promoting degradation of agricultural waste by thermobifida fusca
  • Method for promoting degradation of agricultural waste by thermobifida fusca

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment one, material and method

[0032] 1. Materials and strains

[0033] Cellulose, cellobiose, glucose, xylooligosaccharide, xylose and NaCNBH3 were all purchased from Shanghai Sangon Biotechnology Co., Ltd. (Ltd.Shanghai, China). Xylan, 7-amino-1,3-naphthalene disulfonic acid monohydrate, trichloroacetic acid, dithiothreitol (DTT), iodoacetamide, and trypsin were purchased from Sigma Chemical Co. (St.Louis, MO, USA). All other chemicals used were of analytical grade.

[0034] Thermomyces lanuginosus (Thermomyces lanuginosus) can be conventional Thermomyces lanuginosus in the field, such as the Thermomyces lanuginosus in the Chinese invention patent with patent application number: 201810018260.X. Thermobifidafusca DSM10635 was purchased from the German Culture Collection.

[0035] 2. Solid state fermentation and protein extraction

[0036] Thermomyces lanuginosus and Thermosporium tan were cultured in PDA and Chapei medium at 55°C for 2 days as strains. Mix...

Embodiment 2

[0045] Embodiment 2. Cultivating Thermomyces lanuginosus and Brown Thermophilia on corn stalk medium

[0046] Selecting corn stover as a carbon source to mimic natural habitats. When Thermomyces lanuginosus was cultured alone on the solid medium of corn stover, white mycelia could be detected, while Thermospora lanuginosus could not grow alone on the solid medium of corn stover ( figure 1 A).

[0047] More white mycelia and four bands of xylanase secreted by T. tanensis ( figure 2 Middle bands 2-5) and four bands of cellulase secreted by Thermobista spp. ( figure 2 Middle bands 6-9); that is to say, when Thermomyces lanuginosus and Thermomyces lanuginosus are co-cultured, they can secrete a large amount of xylanase and cellulase ( figure 2 ).

[0048] In corn stover liquid medium, both Thermomyces lanuginosus and Thermospora brownus can secrete xylanase, but compared with pure culture, the xylanase activity of the two co-cultured was not significant. Increase( figure ...

Embodiment 3

[0050] Growth and enzyme secretion characteristics of embodiment three, thermomyces lanuginosus and brown thermophilic bacterium when cultured alone

[0051] Xylan and cellulose are polymers of xylose or glucose and are the most abundant components of plant material. With xylan and cellulose-related substrates (1% xylose, 1% xylooligosaccharides (xylooligosaccharides), 1% xylan, 1% glucose, 1% cellobiose and 1% microcrystalline cellulose ( cellulose), mass / volume) as a carbon source for pure culture. Thermomyces lanuginosus grew on all substrates, but 1% xylose (mass / volume) and 1% xylo-oligosaccharides (mass / volume) inhibited the growth of Thermomyces lanuginosus ( image 3 A).

[0052] The concentration gradient of xylooligosaccharides and their mixtures with cellulose were used to culture Thermomyces lanuginosus and Thermobispodium browni, respectively. For pure cultures of Thermomyces lanuginosus, as the concentration of xylooligosaccharides increased from 0.1% to 1% (...

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Abstract

The invention provides a method for promoting the utilization of a xylan-containing substrate by thermobifida fusca. The thermobifida fusca generates xylanase to decompose xylan to produce xylo-oligosaccharide and xylose. The method comprises a step of controlling the content of xylo-oligosaccharide in a reaction system. Besides, the invention further provides a method for improving the utilization efficiency of a xylan-containing substrate by the thermobifida fusca. The method includes a step of culturing the thermobifida fusca by utilizing a substrate containing xylan; and the thermobifida fusca can produce xylanase and cellulase. And the method further comprises a step of adding thermomyces lanoginosus in the culture process.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for promoting the growth of the brown thermophilic bacterium, in particular to a method for promoting the brown thermophilic bacterium to degrade lignocellulose and promoting the use of brown thermophilic bacterium to agricultural waste Methods. Background technique [0002] Lignocellulosic biomass is the major photosynthetically fixed carbon on Earth, and efficient lignocellulosic degradation has been demonstrated to be key to maintaining the global carbon cycle and bioindustrial development. Wheat, corn, rice, etc., as the main food crops, produce more than 2.2 billion tons of agricultural waste every year. The rational use of these agricultural wastes can not only reduce the pollution caused by incineration, but also turn waste into treasure. as a renewable energy source. Therefore, biodegradation of agricultural waste biomass is a green and potential agricultural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/22C12N9/24C12N9/42C12R1/645
CPCC12N1/14C12N1/22C12N9/2437C12N9/248C12N9/2482C12Y302/01008
Inventor 王禄山史泽露张丽丽陈冠军高培基
Owner SHANDONG UNIV
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