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High-strength polysaccharide-nano-laponite composite microsphere and preparation method thereof

A technology of composite microspheres and lithium algae, which is applied in the direction of microsphere preparation, microcapsule preparation, chemical instruments and methods, etc., can solve the problems of low mechanical strength, inability to withstand high pressure and can only be operated at low flow rates, etc. Improved mechanical strength, wide application space, and improved separation efficiency

Active Publication Date: 2020-03-31
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention overcomes the defects of low mechanical strength of the current polysaccharide microspheres and cannot withstand high pressure and can only be operated at low flow rates. The inorganic nano-lithium algae is used as a physical cross-linking agent, which is tightly combined with the polysaccharide molecular chain through hydrogen bonding to form a uniform The network structure, the nano-lithiumite becomes the physical cross-linking point, supplemented by the subsequent simple chemical cross-linking, and the high-strength polysaccharide-nano-lithium composite microspheres are prepared. The field of rapid separation and purification of molecules has a wide range of applications

Method used

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  • High-strength polysaccharide-nano-laponite composite microsphere and preparation method thereof
  • High-strength polysaccharide-nano-laponite composite microsphere and preparation method thereof
  • High-strength polysaccharide-nano-laponite composite microsphere and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1) Weigh agarose (0.6g), lepidolite (0.06g) and 10ml of ultrapure water into a 100ml round bottom flask as the water phase, and heat with magnetic stirring at 90°C until the solution is clear and transparent. In a 500ml three-neck flask equipped with mechanical stirring, add liquid paraffin (150ml), petroleum ether (50ml), Span 80 (7.2g) and Tween (0.8g) and heat up to 80°C as the oil phase. Add the above-prepared water phase into the oil phase for emulsification at 350 rpm for 40 min to obtain a white W / O emulsion.

[0028] 2) Slowly add a mixed solution of 4ml of crosslinking agent epichlorohydrin and 2ml of 45% NaOH dropwise to the above emulsion, further carry out crosslinking reaction at 80°C, and wash microspheres with petroleum ether, ethanol and deionized water successively after reacting for 8h. spheres to obtain agarose-nanometer lithium composite microspheres with an average particle size of 85 μm. After crosslinking, the composite microspheres were stored i...

Embodiment 2

[0030] 1) Weigh dextran T20 (1.0g), lepidolite (0.03g) and 25ml of ultrapure water into a 100ml round bottom flask as the water phase, and magnetically stir until the solution is clear and transparent. In a 250ml three-neck flask equipped with mechanical stirring, add olive oil (75ml) and PO 500 (2.25g) and heat up to 20°C as the oil phase. Add the above-prepared water phase into the oil phase for emulsification at 1000 rpm for 10 min to obtain a white W / O emulsion.

[0031] 2) Slowly add a mixed solution of 5ml of cross-linking agent ethylene glycol diglycidyl ether and 3ml of 65% KOH dropwise to the above-mentioned emulsion, and further carry out cross-linking reaction at 10°C. After 24 hours of reaction, use petroleum ether, ethanol and deionized The microspheres are washed with water to obtain dextran-nanometer lithium composite microspheres with an average particle size of 23 μm. After crosslinking, the composite microspheres were stored in 20% ethanol at 4°C.

Embodiment 3

[0033] 1) Weigh agarose (1.0g), lithium diatomaceous earth (0.1g) and 25ml of ultrapure water into a 100ml round bottom flask as the water phase, and heat with magnetic stirring at 90°C until the solution is clear and transparent. In a 250ml three-neck flask equipped with mechanical stirring, add chlorobenzene (75ml) and Span 80 (3g) and heat up to 60°C as an oil phase. Add the above-prepared water phase into the oil phase for emulsification at 450 rpm for 100 min to obtain a white W / O emulsion.

[0034] 2) Slowly add a mixed solution of 6ml of cross-linking agent epichlorohydrin and 3ml of 40% NaOH dropwise to the above emulsion, further carry out cross-linking reaction at 60°C, and wash the microgel with petroleum ether, ethanol and deionized water successively after reacting for 2 hours. spheres to obtain agarose-nanometer lithium composite microspheres with an average particle size of 125 μm. After crosslinking, the composite microspheres were stored in 20% ethanol at 4°C. ...

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Abstract

The invention relates to the field of organic-inorganic composite material preparation and especially relates to a high-strength polysaccharide-nano-laponite composite microsphere and preparation method thereof. The nano-laponite has abundant hydroxyl on the surface, so that through the hydrogen bonds, the nano-laponite and the polysaccharide macromolecules are combined to achieve great physical crosslinking effect; and with following chemical crosslinking, the polysaccharide-nano-laponite composite microsphere with excellent performances is prepared. A protein adsorption test and a pressure-flow speed curve test prove that the composite microsphere has good biocompatibility, wherein operation flow speed is increased by more than two times than that of single polysaccharide microspheres. The composite microsphere has extensive application potential in the field of enzyme immobilization, cell culture, and quick separation and purification of biomacromolecules.

Description

technical field [0001] The invention relates to the field of preparation of organic-inorganic composite materials, in particular to a high-strength polysaccharide-nanometer lithium composite microsphere and a preparation method thereof. Background technique [0002] With the rapid development of biotechnology, people have synthesized a large number of biological macromolecules such as proteins and nucleic acids through cell fusion and DNA recombination. These active substances often exist in the form of complex mixtures, and their large-scale separation and purification is a major challenge we face. Chromatography is an effective means to separate and purify biomacromolecules, and choosing a bioseparation medium with high column efficiency, large column capacity, fast separation speed and low activity loss is the key to ensure separation efficiency. In order to avoid the loss of biomacromolecular activity during the separation process, the separation medium is required to h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/14B01J20/24B01J20/28B01J20/30
CPCB01J13/14B01J20/24B01J20/10B01J20/28016
Inventor 曲剑波李丹丹陈梦琦彭文舒蔺洋洋李刚锋朱冰琪李静张晓云
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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