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A method for extracting and separating active substances of black cumin seed oil

A technology for Nigella sativa seeds and active substances is applied in the field of extraction and separation, which can solve the problems of inability to guarantee extraction efficiency and biological activity of extracts, and achieve good extraction effect, optimized extraction process, and good effect of solvent biological activity.

Active Publication Date: 2021-10-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The research on the extraction and separation methods of extracting Nigella seed oil from Nigella seeds mainly includes traditional pressing method, steam distillation method, supercritical fluid extraction method, etc. to optimize the extraction process of seed oil or volatile oil, but none of them can guarantee the extraction Efficiency and biological activity of the extract

Method used

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  • A method for extracting and separating active substances of black cumin seed oil
  • A method for extracting and separating active substances of black cumin seed oil
  • A method for extracting and separating active substances of black cumin seed oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Extraction of antioxidant active substances in black grain oil

[0042] (1) Preparation of low-molten solvent

[0043] The cholinergic acid and the acetylic acid were placed in a molar ratio of 1: 4 in a cover, and the water bath was heated to 70 ° C after vibration, and the uniform and stable colorless clarified transparent liquid was continuously shaped. After the preparation, it can be placed in a spare, and the liquid is still liquid under room temperature.

[0044] (2) Extraction of low coefficient melting solvent

[0045] The low-molten solvent water bath is heated to 50 to 60 ° C to reduce the viscosity of the low-molten solvent, which is also conducive to increasing extraction efficiency. In order to save energy more reasonable arrangements, the prepared low-melting solvent can be cooled to 50 to 60 ° C, which is directly applied to extraction. 1.5 ml of centrifuge tube was taken, 0.5 ml of black grotto oil was added, then a low-molten solvent was 0.5 ml....

Embodiment 2

[0048]Example 2: Preparation of Different Low Combinated Solvents

[0049] The choline choline is used as a hydrogen bond receptor, and the different hydrogen bond donor is formulated into different low-molten solvents, most of which cannot be used as a solvent at extraction temperature due to excessive viscosity or too high of excessive gas. For example, chlorine choline-sorbitol, also have other substances as a hydrogen bond receptor, such as a betaine of a hydrochloride, but the low-molten solvent prepared by the betaine of a hydrochloride is too high without being used as an extraction solvent, and therefore Chloride choline as a hydrogen bond receptor, formulated into different low-molten solvents to screen.

[0050] The low-molten solvent formed by chloride-acecyclosilic acid was selected from 1: 4, and choline-1,2 propylene glycol was prepared by a proportion of 1: 4. Chochloride - Madmic acid-formulated low-molten solvents made of 1: 1 as a proportion of low-melting solven...

Embodiment 3

[0066] Example 3: Optimization Experiment of Extraction Process of Low Common Solvent

[0067] The optimal extraction process is screened by a single factor experiment method, examining different low-melting solvents (chloride and acetyl propacionic acid molar ratio 1: 4, choline and 1,2-propylene glycol ratio 1: 4, chlorination Choline and glycerol molar ratio 1: 2, chloride and apple acid molar ratio 1: 1); low-melting solvent ratio (choline choline and acetyl propacionic acid molar ratio of 1: 2, 1: 4, 1: 6, 1: 8, 1: 10); Ultrasonic extraction time (10, 20, 30, 40, 50 minutes); extraction temperature (30, 40, 50, 60, 70 ° C); extract oil and low Follow solvent ratio (1: 1, 1: 2, 1: 4, 1: 6, 1: 8; 4: 1, 2: 1, 1: 1, 1: 2, 1: 4) Extraction of low-molten solvent The effects of the effects were removed by DPPH free radical removal activity as an indicator.

[0068] When a factor is a variable, the remaining factors is a low-molten solvent having a cholinergic acid and acetyl propyle...

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Abstract

The invention discloses a method for extracting and separating active substances of black cumin seed oil, which is to prepare a deep eutectic solvent with choline chloride and levulinic acid, and heat it for standby; adding the deep eutectic solvent into black cumin seed oil, Ultrasonic, extraction; centrifugation, take the DES phase. The extraction of Nigella seed oil by using deep eutectic solvent has many advantages that other organic solvents do not have. It has the advantages of good activity and broad application prospects.

Description

Technical field [0001] The present invention is belonging to the field of extraction separation, and more particularly to a method of extracting an isolated active substance from a black grain oil. Background technique [0002] Black grass is a born herbaceous plant, which is often referred to as black grass. The plant is native to Southwest Asia, North Africa and South Europe, which also planted in many Mediterranean and Middle East countries, such as Iran, Pakistan, India, Saudi Arabia, Syria and Turkey, have also distributed in Xinjiang in China. Its plants and seeds have been widely used as an edible herbal plant and condiment. It is also used for medical purposes. Black grill oil is widely used in traditional treatment of diseases such as rheumatism, acne, jaundice, indigestion, worm and skin disease. Studies on animals have shown that the oil has anti-hypertension, anti-diabetic, antibacterial, anti-cancer, diuretic, analgesic, anti-inflammatory and antioxidant. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61P39/06A61K131/00
CPCA61K36/71A61K2236/333A61P39/06
Inventor 刘学伏琳玥杨成
Owner JIANGNAN UNIV
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