Method for simultaneously detecting four protein-bound uremic toxins in blood sample

Abstract

The invention discloses a method for simultaneously detecting four protein-bound uremic toxins in a blood sample. The method comprises the following steps of pretreating the blood sample, and carryingout ultra-high performance liquid chromatography-tandem mass spectrometry detection on the treated sample to be detected, wherein the pretreatment process of the blood sample comprises the followingsteps of mixing the blood sample with a precipitant, carrying out centrifugal separation, adding the separated supernatant into the formic acid-containing water, and uniformly mixing to obtain the sample to be detected, wherein the precipitant is a mixed solution of acetonitrile and ethanol, the volume ratio of acetonitrile to ethanol is 2: (0.9-1.1), and the four protein binding uremia toxins areCMPF, IS, PCS and HA. According to the invention, the detection time of the four protein-bound uremic toxins can be shortened.

Description

technical field [0001] The invention belongs to the technical field of detection of protein-bound uremic toxins, and relates to a method for simultaneously detecting four kinds of protein-bound uremic toxins in blood samples. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Protein-bound uremic toxins (protein-bound uremic toxins, PBUT) are an important class of uremic toxins, which are mainly produced by the decomposition of amino acids by intestinal bacteria and secreted by organic anion channels in the basolateral membrane of renal proximal tubular epithelial cells Enter the renal tubules, and finally excreted with urine, so the concentration of serum protein-bound urem...

AI Technical Summary

Problems solved by technology

The type of immunoassay detection is relatively single, and the antibodies used in the detection can also recognize other protein-bound uremic toxin derivatives, and the antibody-antigen reaction is usually less than 100%, so the results vary greatly and the specificity is poor, so it is reliable in clinical detection not tall

Due to the limited detection limit of liquid chromatography and the complex components of plasma / serum, magazines seriously affect the accuracy of test results during quantitative analysis, so it is not suitable for clinical popularization

[0006] Ultra-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) can avoid the above-mentioned defects, but the inventors of the present invention have found through research that the current detection time of various protein-bound uremic toxins using HPLC-MS / MS longer

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