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Artificial cultivation method of Maowo fungi

A technology of artificial cultivation and chrysalis, which is applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve problems such as unsatisfactory effect, low output, and no successful artificial cultivation of chrysalis

Inactive Publication Date: 2020-03-24
CANGZHOU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chrysalis generally parasitizes the base of thatch plants, solitary or in clusters, and it is easy to grow after continuous light rain in summer, but due to the limitation of natural resource conditions such as ambient temperature, humidity, climate, and thatch land conditions, the output is small and unstable, which is difficult to meet People's needs, especially the dry mushroom products have become treasures for relatives and friends
[0003] At present, the chrysalis grows wildly, and the breeding and cultivation of the chrysalis through the artificial breeding method of conventional edible fungus is often not ideal; so far, there is no report on the successful artificial cultivation of the chrysalis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Carry out the artificial cultivation of chrysalis as follows:

[0027] (1) In the saline-alkali thatched grass field, select the wild Pseudomonas spp. strains with typical characteristics and good growth, and use the tissue separation method to obtain multiple mycelium blocks near the junction of the fruiting body stipe and gills, each of which is the size of a mung bean grain , immediately used in the inoculation operation of step (2) as the original species of chrysalis;

[0028] (2) Get 600 g of chopped saline-alkali fresh grass roots, add 2000 g of water and boil until the water boils and maintain a slight boil for 30 minutes, filter, and the resulting filtrate is the grass root extract; add 200 g of potato slices, 20 g of glucose, and agar to the grass root extract 20g, boil on high heat until the water boils and keep boiling on medium heat for 20min, filter with four layers of gauze, and obtain 1000ml of filtrate after cooling, which is the coriander root-PDA comp...

Embodiment 2

[0033] After the fruiting body described in Example 1, that is, the fruiting body of the chrysanthemum, continue to control the temperature of the greenhouse at 24-28° C. and the relative humidity of 85-90%, keep proper ventilation and shading, and the fruiting body of the chrysanthemum can be harvested from the harvest. The cultivation substrate at the end of the 4-6cm wide thin soil belt continues to grow and develops to a mature state that can be harvested; 4 crops can be harvested continuously; together with the first crop, the cultivation bed per square meter The average total yield of M. scutellaria was 1.35kg in total.

[0034] Repeat the preparation steps of the autoclaved bag material in step (3) of Example 1, and prepare 6 bags of sterilized bag material in total for the following use when needed.

Embodiment 3

[0036]Carry out the operation of each step synchronously with embodiment 1 and artificially cultivate the chrysalis, the difference comprises: step (1) selects the wild chrysoplastum bacterial strain with typical character and growing way in saline-alkali-free thatched grass land, adopts tissue separation method to obtain fruiting bodies The mycelium pieces near the joints of the stipe-gills are used as the original species of the chrysalis; step (2) adopts the fresh chrysalis root of the saline-alkali-free land to prepare the chrysanthemum root-PDA composite medium.

[0037] As a result, the preparation effect of the mother species and cultivars of the chrysalis in steps (2), (3), and the growth and output effects of the chrysalis in steps (4), (5) are slightly better than that of Example 1; The average yield of the first crop of Mushrooms in the cultivation bed is 0.51kg. After harvesting the first batch, continue to control the temperature of the greenhouse at 24-28°C and th...

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Abstract

The invention provides an artificial cultivation method of Maowo fungi, which comprises the following steps: (1) selecting a wild Maowo fungi strain, and separating to obtain an Maowo fungi sporocarpas an Maowo fungi stock; (2) preparing a Imperata cylindrica root-PDA composite culture medium, placing the Imperata cylindrica root-PDA composite culture medium in a sterile culture container, inoculating of stock seeds, and culturing mother seeds; (3) uniformly mixing crushed fresh Imperata cylindrica, cottonseed hull, wheat bran and lime, bagging, sterilizing, cooling, inoculating of the Maowofungi mother strain, and culturing to obtain a Maowo fungi cultivar; (4) uniformly mixing fresh Imperata cylindrica, cottonseed hull, wheat bran and lime, adding water, piling and fermenting to prepare a culture medium; paving the culture medium on a greenhouse ground culture bed, sowing Maowo fungi cultivar, covering a film, and controlling greenhouse conditions to enable Maowo fungi to grow; (5)after Maowo fungi hyphae grow all over, removing the film, covering with strip-shaped fine soil belts, and spraying water on the soil covering layer until the soil covering layer is wet and thoroughly watered; and controlling greenhouse conditions, and harvesting fruiting bodies when the fruiting bodies of the Maowo fungi are flat so as to obtain Maowo fungi products.

Description

Background technique [0001] The large area of ​​saline-alkaline wasteland in Bohai Bay is mainly dominated by Imperata praecox, and the associated plants include reeds, bicolor limonium, velvetleaf, radish, zoysia, sinensis limonium, apocynum, foxtail, thistle, tamarisk etc., with a total coverage of about 70%; the Imperata coronata community is the last herbaceous vegetation in the succession from saline meadow vegetation to woody plants. In the saline-alkali thatched land, there is a unique edible fungus - the chrysalis. The local people fight to pick it in the rain during the season when the chrysalis grows. They either eat it fresh or dry it in the sun for storage. [0002] Mycelia is composed of two parts: mycelium and fruiting body. The mycelium is the vegetative organ, which is white and filamentous; The diameter of the cover is about 0.5-2cm. The chrysalis generally parasitizes the base of thatch plants, solitary or in clusters, and it is easy to grow after continuou...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20A01G18/40
CPCA01G18/00A01G18/20A01G18/40
Inventor 孔德平王增池阎旭东
Owner CANGZHOU ACAD OF AGRI & FORESTRY SCI
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