Artificial cultivation method of Maowo fungi
A technology of artificial cultivation and chrysalis, which is applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve problems such as unsatisfactory effect, low output, and no successful artificial cultivation of chrysalis
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Embodiment 1
[0026] Carry out the artificial cultivation of chrysalis as follows:
[0027] (1) In the saline-alkali thatched grass field, select the wild Pseudomonas spp. strains with typical characteristics and good growth, and use the tissue separation method to obtain multiple mycelium blocks near the junction of the fruiting body stipe and gills, each of which is the size of a mung bean grain , immediately used in the inoculation operation of step (2) as the original species of chrysalis;
[0028] (2) Get 600 g of chopped saline-alkali fresh grass roots, add 2000 g of water and boil until the water boils and maintain a slight boil for 30 minutes, filter, and the resulting filtrate is the grass root extract; add 200 g of potato slices, 20 g of glucose, and agar to the grass root extract 20g, boil on high heat until the water boils and keep boiling on medium heat for 20min, filter with four layers of gauze, and obtain 1000ml of filtrate after cooling, which is the coriander root-PDA comp...
Embodiment 2
[0033] After the fruiting body described in Example 1, that is, the fruiting body of the chrysanthemum, continue to control the temperature of the greenhouse at 24-28° C. and the relative humidity of 85-90%, keep proper ventilation and shading, and the fruiting body of the chrysanthemum can be harvested from the harvest. The cultivation substrate at the end of the 4-6cm wide thin soil belt continues to grow and develops to a mature state that can be harvested; 4 crops can be harvested continuously; together with the first crop, the cultivation bed per square meter The average total yield of M. scutellaria was 1.35kg in total.
[0034] Repeat the preparation steps of the autoclaved bag material in step (3) of Example 1, and prepare 6 bags of sterilized bag material in total for the following use when needed.
Embodiment 3
[0036]Carry out the operation of each step synchronously with embodiment 1 and artificially cultivate the chrysalis, the difference comprises: step (1) selects the wild chrysoplastum bacterial strain with typical character and growing way in saline-alkali-free thatched grass land, adopts tissue separation method to obtain fruiting bodies The mycelium pieces near the joints of the stipe-gills are used as the original species of the chrysalis; step (2) adopts the fresh chrysalis root of the saline-alkali-free land to prepare the chrysanthemum root-PDA composite medium.
[0037] As a result, the preparation effect of the mother species and cultivars of the chrysalis in steps (2), (3), and the growth and output effects of the chrysalis in steps (4), (5) are slightly better than that of Example 1; The average yield of the first crop of Mushrooms in the cultivation bed is 0.51kg. After harvesting the first batch, continue to control the temperature of the greenhouse at 24-28°C and th...
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