Degradable microspheres simultaneously loaded with anti-cancer drug and active factor and provided with micro-nano structure, as well as preparation method and application of degradable microspheres
A micro-nano structure and active factor technology, applied in the field of biomedical materials, can solve the problems of not specifying the specificity of the morphology and structure of microspheres and nanoparticles, and not listing the results of cell experiments, so as to improve the quality of life and slow down the diffusion. , the effect of reducing side effects
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[0028] The present invention firstly provides a method for preparing degradable microspheres with micro-nano structure simultaneously loaded with anticancer drugs and active factors, comprising the following steps:
[0029] Step 1: using degradable polymers as raw materials to prepare polymer microspheres loaded with active factors; the degradable polymers include aliphatic polyester or polyester polyether copolymers;
[0030]Step 2: Using biomedical polymers as raw materials, prepare micro-nano particles loaded with anticancer drugs; the biomedical polymers include aliphatic polyesters, polyester polyether copolymers, polystyrene, polyacrylates, At least one of polymethacrylate, polyacrylonitrile or polyisobutylene;
[0031] Step 3: Composite polymer microspheres loaded with active factors and micro-nano particles loaded with anti-cancer drugs, so that micro-nano particles are loaded on the polymer microspheres, and a micro-nano structure loaded with anti-cancer drugs and act...
Embodiment 1
[0052] Example 1 Preparation of micro-nano structured porous PLGA microspheres loaded with paclitaxel and RGD
[0053] Prepare 2.5 mL of 10% (g / mL) PCL / CHCl 3 solution, then add 100 μg paclitaxel, above-mentioned solution and 5ml, 0.5% (g / mL) PVA aqueous solution are ultrasonic 1min under 200w to obtain emulsion, above-mentioned emulsion is added in the PVA aqueous solution of 100ml 0.5% (g / mL), under 700rpm Continue to stir for 5h until CHCl 3 After volatilization, the paclitaxel-embedded PCL micro-nanoparticles were obtained, washed and collected. The prepared PCL nanoparticles were further soaked in excess 0.1M NaOH solution for 30 min, washed and filtered repeatedly to remove excess NaOH on the surface of the nanoparticles.
[0054] Preparation of PLGA / CH 2 Cl 2 The solution is used as the oil phase (O), the solution is placed under the homogenizer and immersed in an ice-water bath to maintain the temperature, and the prepared inner water phase (W) is added to the oil ...
Embodiment 2
[0056] Example 2 Preparation of micro-nano structured PCL-PEG microspheres loaded with camptothecin and REDV
[0057] First prepare 2.5mL, 10% (g / mL) of PCL-PEG-NH 2 / CHCl 3 Solution, then add 100 μ g camptothecin, above-mentioned solution and 5ml, the PVA aqueous solution of 0.5% (g / mL) obtain emulsion under 200w supersonic 1min, above-mentioned emulsion is added in the PVA aqueous solution of 100ml 0.5% (g / mL), Continue stirring at 700rpm for 5h until CHCl 3 Get PCL-PEG-NH after volatilization 2 Micro-nanoparticles, washed and collected.
[0058] Preparation of PCL-PEG-COOH / CH 2 Cl 2 The solution is used as the oil phase (O), the solution is placed under the ultrasonic probe and immersed in an ice-water bath to maintain the temperature, and the prepared internal water phase (W) is injected into the oil phase. 1 ), and obtain emulsion (E 1 ), then E at room temperature 1 Add dropwise to the external aqueous phase (W 2 ), continue to stir for 2h after the dropwise add...
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