A bacteriostatic fusion Weissella mp4 bacterial strain and its application

A technology of Weissella and bacteriostasis, which is applied in the field of fusion of Weissella MP4 strains, can solve problems affecting sustainable and healthy agricultural development, environmental pollution, and reduced control efficiency, and achieve good market application prospects

Active Publication Date: 2020-05-26
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Botrytis cinerea and Fusarium oxysporum reproduce very quickly and produce a large number of conidia, so they are easy to mutate and produce drug resistance. Resistance to various fungicides such as fungicides, carbamates, etc., not only leads to the reduction of the control effect, but also leads to the continuous increase of the amount of pesticides used
Excessive use of chemical agents not only causes excessive pesticide residues in agricultural products, endangers the health of consumers, but also causes environmental pollution, destroys ecological balance, and affects the sustainable and healthy development of agriculture.

Method used

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  • A bacteriostatic fusion Weissella mp4 bacterial strain and its application
  • A bacteriostatic fusion Weissella mp4 bacterial strain and its application
  • A bacteriostatic fusion Weissella mp4 bacterial strain and its application

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Embodiment 1

[0025] 1. Isolation and screening of strains

[0026] On June 15, 2017, 30 grape root samples were collected in Daze Mountain, Qingdao. The samples were transferred to a sterilized mortar for grinding, diluted with sterile water to 10ml, and then diluted to 10ml with sterile water. -3 -10 -4 Take 200ul of PDA-coated plates (200g / l potato, 20g / l glucose, 20g / l agar, natural pH), and culture them statically at 26°C.

[0027] Pick a single colony and inoculate a single bacterial colony in MRS (peptone 10.0g / l, beef extract 10.0g / l, yeast extract 5.0g / l, glucose 20.0g / l, triammonium citrate 2.0g / l , sodium acetate 5.0g / l, MgSO 4 0.1g / l, MnSO 4 0.05g / l, K 2 HPO 4 2.0g / l, pH 6.5) in liquid culture medium at 26°C for 48h, then centrifuged at 12,000rpm at 4°C, and the supernatant was filtered through a filter with a diameter of 0.22um for later use.

[0028] The filtrate was added to the MRS medium according to different proportions, and the conidia of Botrytis cinerea and Fus...

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Abstract

The invention discloses a fused Weissella MP4 strain with antibacterial effect and its application. The present invention obtains the MP4 strain with inhibitory effect on plant wilt caused by Botrytis cinerea and Fusarium oxysporum through screening. The specific taxonomy named fusion Weissella MP4 Weissella confuse MP4, preserved in China Center for Type Culture Collection, its preservation number is CCTCC NO: M 2019701. The present invention proves that the MP4 bacterial strain obtained by the present invention has a stronger inhibitory effect on Botrytis cinerea and Fusarium oxysporum through experiments on Botrytis cinerea and Fusarium oxysporum spores, germ tube elongation and isolated leaves, and can It is prepared as a biocontrol agent for preventing and treating Botrytis cinerea and Fusarium wilt, which has a good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a fusion Weissella MP4 strain with antibacterial effect and application thereof. Background technique [0002] Botrytis cinerea is an important plant pathogenic fungus, which is widely distributed in the world and is known as the second most destructive plant pathogen. crops, can cause fruit and vegetable botrytis. The disease can occur not only during the growing season of the host plant, but also during the storage of agricultural products, so the damage is serious. Especially in recent years, the large-scale promotion of protected cultivation methods has led to wide sources of infection and rapid spread of the fungus. Botrytis cinerea is becoming more and more serious, and its occurrence and prevalence often cause serious economic losses. According to reports, the occurrence of Botrytis cinerea in northern facilities is generally serious, and the economic loss c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 王光远高明明梁文星宋丽敏
Owner QINGDAO AGRI UNIV
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