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Method for measuring content of atractylis in joint arthralgia-relieving pill

A determination method, the technology of Kebi pills, is applied in the field of quality inspection of traditional Chinese medicine, and can solve problems such as the determination of atractylodes components

Inactive Publication Date: 2020-02-14
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing quality inspection method of Joint Kebi Pills does not measure the content of Atractylodes atractylodes. The content determination project is very necessary

Method used

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  • Method for measuring content of atractylis in joint arthralgia-relieving pill
  • Method for measuring content of atractylis in joint arthralgia-relieving pill
  • Method for measuring content of atractylis in joint arthralgia-relieving pill

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Effect test

Embodiment 1

[0072] Embodiment 1 of the present invention: a method for determining the content of atractylodes in joint Kebi pills is as follows:

[0073] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use acetonitrile: 0.5% phosphoric acid aqueous solution = 40:60 as mobile phase; detection wavelength is 254nm, flow rate is 1.0mL / min , the column temperature is 35°C; the number of theoretical plates should not be less than 5000 based on the peak of Atractylodes atractylodis;

[0074] (2) Preparation of reference substance solution: take an appropriate amount of Atractylodes atractylodis reference substance, accurately weighed, add methanol to make 1mL solution containing 0.1mg, and set aside;

[0075] (3) Preparation of the test solution: Accurately weigh 2.0g of Joint Kebi Pills, accurately add 70% ethanol 100mL, weigh, ultrasonically extract for 1h, make up the weight with ethanol, filter; accurately measure and continue fil...

Embodiment 2

[0077] Embodiment 2 of the present invention: a method for determining the content of atractylodes in Joint Kebi Pills is as follows:

[0078] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; acetonitrile: water = 40:60 is used as mobile phase; detection wavelength is 254nm, flow rate is 1.0mL / min, column temperature The temperature is 35°C; the number of theoretical plates should not be less than 5000 based on the peak of Atractylodes atractylodes;

[0079] (2) Preparation of reference substance solution: take an appropriate amount of Atractylodes atractylodis reference substance, accurately weighed, add methanol to make 1mL solution containing 0.1mg, and set aside;

[0080] (3) Preparation of the test solution: Accurately weigh 2.0 g of Joint Kebi Pills, accurately add 100 mL of methanol, weigh it, extract by ultrasonic or reflux for 1 h, make up the weight with methanol, and filter; accurately measure 50 mL of ...

Embodiment 3

[0082] Embodiment 3 of the present invention: a method for determining the content of atractylodes in joint Kebi pills is as follows:

[0083] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol:0.05% glacial acetic acid=60:40 is used as mobile phase; column temperature is 40°C; detection wavelength is 270nm; The flow rate is 1.5mL min -1 ;The number of theoretical boards should not be less than 5000 based on the calculation of Atractylodes atractylodes;

[0084] (2) Preparation of reference substance solution: take an appropriate amount of Atractylodes atractylodis reference substance, accurately weighed, add methanol to make 1mL solution containing 0.1mg, and set aside;

[0085] (3) Preparation of the test solution: Accurately weigh 2.0 g of Joint Kebi Pills, accurately add 100 mL of methanol, weigh it, extract by ultrasonic or reflux for 1 h, make up the weight with methanol, and filter; accurately measu...

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Abstract

The invention discloses a method for measuring content of atractylis in a joint arthralgia-relieving pill. The method is a high performance liquid chromatography method which takes an atractylis reference substance as a reference and takes methanol, water, 0.01-5% phosphoric acid aqueous solution, 0.01-5% glacial acetic acid and 0.01-0.5% formic acid aqueous solution in a ratio of (50-70): (50-30)or acetonitrile, water, 0.01-5% phosphoric acid aqueous solution, 0.01-5% glacial acetic acid and 0.01-0.5% formic acid aqueous solution in a ratio of (20-50): (80-50) as a mobile phase. The method disclosed by the invention is high in specificity, high in precision, high in repeatability, high in recovery rate, high in stability and accurate in measurement result, the aim of effectively controlling the quality of medicines is achieved, and the stability of the product quality and the safety and effectiveness of clinical medication are ensured.

Description

technical field [0001] The invention relates to a method for determining the content of atractylodes in Joint Kebi Pills, belonging to the technical field of traditional Chinese medicine quality detection. Background technique [0002] Joint Kebi Pills have the effects of expelling wind and cold, activating collaterals and relieving pain. For arthritis, sore limbs, poor stretching and other diseases. Modern pharmacological experiments have proved that Joint Kebi Pills have antispasmodic effect on rabbit ileum; it has analgesic, sedative and anti-inflammatory effects at the same time: Pharmacological studies have shown that Joint Kebi Pills can significantly prolong the time caused by hot plate method in mice. The pain reaction time of the animals is shown to have obvious analgesic effect. [0003] Atractylodes atractylodis has anti-inflammatory, antibacterial, antiviral and other effects. Modern pharmacological research shows that it is one of the active ingredients of tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N2030/027
Inventor 杨淙李苹孔令忠欧凌云
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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