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Plasminogen measuring kit and preparation method thereof

A technology of plasmin and kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection accuracy and sensitivity, and achieve the effects of convenient instrument measurement, stable environment, and increased turbidity

Inactive Publication Date: 2020-02-07
浙江爱康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The detection of plasminogen is mainly immunoturbidimetric method. At present, for example, the patent with the notification number CN103743911B discloses a fibronectin assay kit for measuring the content of fibronectin in serum and its use method, which overcomes the traditional The kit has the disadvantages of low sensitivity and low detection accuracy, and another kit and preparation method are proposed below

Method used

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  • Plasminogen measuring kit and preparation method thereof
  • Plasminogen measuring kit and preparation method thereof
  • Plasminogen measuring kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Reagent R1 includes the following raw materials:

[0050] 100mmol / L citrate buffer;

[0051] 10mmol / L sodium azide;

[0052] PEG 6000 at 0.8 mmol / l;

[0053] 2g / L stachyose;

[0054] 1.6g / L sodium fructose diphosphate;

[0055] 0.25g / L sodium hexametaphosphate;

[0056] 50mmol / L of sodium chloride;

[0057] 5g / L bovine serum albumin BSA;

[0058] The reagent R2 comprises the following raw materials:

[0059] 100mmol / L citrate buffer;

[0060] 0.85% (by mass volume ratio) of latex particles;

[0061] 10mmol / L sodium azide;

[0062] 1.8mg / mL goat anti-human plasminogen antibody;

[0063] 5g / L bovine serum albumin BSA.

[0064]The preparation method is as follows: it includes the following steps: step S1, weighing each component of the reagent R1, stirring evenly, standing still, filtering and removing impurities; Add other components. Step S2, weighing each component of the reagent R2, stirring evenly, standing still, filtering and removing impurities; in the...

Embodiment 2

[0071] Accuracy verification test: the plasminogen detection reagent of embodiment 1 is used as an experimental group, and a kind of plasminogen detection reagent with good accuracy and good stability that has been recognized on the market is used as a control group for detection. Ten clinical serum samples were tested, and the test results are shown in Table 1. The results showed that the correlation coefficient of the two kits was 0.9979, indicating that the correlation between the two kits is relatively good. It proves that the added and changed components of the kit of the present invention will not affect its accuracy, and the kit still maintains a good accuracy.

[0072] Table 1 is the experimental result of the accuracy of reagents of the present invention and matched group reagents;

[0073]

Embodiment 3

[0075] Linear correlation verification test: Select a high-value sample with a plasminogen content of 420 mg / L, serially dilute it with normal saline, and prepare 7 samples with different concentrations, the concentrations are 420 mg / dL, 350 mg / dL, and 280 mg / dL. dL, 210mg / dL, 140mg / dL, 70mg / dL, 0mg / dL. The reagents of Example 1 and the control group were used for detection respectively. The samples of each concentration were measured three times, and the average values ​​were taken respectively. The detection results are shown in Table 2.

[0076] Theoretical concentration (mg / L) Embodiment 1 reagent (mg / L) Control group reagent (mg / L) 0 -0.21 0.19 70 72.34 74.93 140 134.63 149.86 210 223.26 218.94 280 288.49 273.51 350 355.97 341.73 420 431.74 436.94 Correlation coefficient R 0.9995 0.9982

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Abstract

The invention relates to a plasminogen measuring kit and a preparation method thereof. the measuring kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the raw materials of80-120 mmol / L of citrate buffer solution, 8-15 mmol / L of preservative, 0.5-1.0mmol / L of surfactant, 0.8-3g / L of stachyose, 0.8-3g / L of fructose diphosphate, 0.05-0.5g / L of sodium hexametaphosphate, 40-60mmol / L of sodium chloride and 4-6g / L of bovine serum albumin (BSA); and the reagent R2 comprises the raw materials of 80-120 mmol / L of citrate buffer solution, 0.5-1% of latex particles (by mass volume), 8-15mmol / L of sodium azide, 1.8mg / ml of goat anti-human plasminogen antibody and 4-6g / L of bovine serum albumin (BSA). The invention provides a fibronectin measuring kit with high sensitivity and high detection precision and wide application range, and a preparation method thereof.

Description

Technical field: [0001] The invention relates to the technical field of medical detection reagents, in particular to a plasminogen assay kit and a preparation method thereof. Background technique: [0002] Plasmin (PL) is produced by PLG under the action of its activator (PA), and is the most direct factor leading to fibrin degradation. Under physiological conditions, PL, PLG, t-PA, etc. are combined on the surface of vascular endothelial cells. Once a small amount of fibrin is formed, PLG is activated into PL, which degrades fibrin locally to avoid thrombus formation and ensure blood flow. unobstructed. [0003] Immunoscattering turbidimetry is that when light of a certain wavelength is irradiated along the horizontal axis and encounters antigen-antibody complex particles when passing through the solution, the light is refracted by the particles and deflected, and the angle of light deflection is related to the wavelength of the emitted light and the antigen-antibody compl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573G01N2333/96411
Inventor 黄益峰
Owner 浙江爱康生物科技有限公司
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