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Antifungal pharmaceutical composition consisting of periplaneta americana extract and antifungal drug

An antifungal drug, the technology of Periplaneta americana, applied in the field of medicine, can solve the problems of fungal drug resistance and decline in clinical cure rate, and achieve the effect of reducing pain

Inactive Publication Date: 2020-02-07
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs currently used clinically for the treatment of vulvovaginal candidiasis, such as azole antifungal drugs, traditional Chinese medicine lotion, etc., have been used in large quantities for a long time, causing the fungus to develop drug resistance, and the clinical cure rate has declined.

Method used

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  • Antifungal pharmaceutical composition consisting of periplaneta americana extract and antifungal drug
  • Antifungal pharmaceutical composition consisting of periplaneta americana extract and antifungal drug
  • Antifungal pharmaceutical composition consisting of periplaneta americana extract and antifungal drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of Periplaneta americana extract and its antifungal activity test in vitro;

[0040] The Periplaneta americana, bacterial strains and reagents used in the examples are all commercially available.

[0041] 1. Preparation of Periplaneta americana Extract

[0042] 1.1 Raw material powder preparation

[0043] Adults of Periplaneta americana were collected and inactivated at -80°C, washed and air-dried with 75% ethanol, and stored in a -80°C refrigerator for later use. Weigh an appropriate amount of adult Periplaneta americana, grind it into powder in liquid nitrogen, freeze it in a -80°C refrigerator, then freeze-dry it in a vacuum freeze dryer to form a powder, mix it thoroughly and store it in a 4°C refrigerator for later use .

[0044] 1.2 Preparation of petroleum ether extract

[0045] 1.2.1 Take 500g of Periplaneta americana powder, put it in a conical flask, add 1000mL of petroleum ether, sonicate for 15min, and soak for 24h. After stirring with a gla...

Embodiment 2

[0079] Anti-Candida albicans activity of Kangfuxin liquid combined with fluconazole in vitro;

[0080] The Kangfuxin solution, bacterial strains and reagents used in the examples are all commercially available.

[0081] 1. Determination of antibacterial activity of Kangfuxin solution combined with fluconazole by microdilution method

[0082] Micro-dilution method, on a 96-well culture plate, the positive drug fluconazole is stored at a concentration of 50 mg / mL, diluted to an initial concentration of 1 mg / mL, Kangfuxin solution is used as the original solution, and diluted with Sabouraud’s liquid medium during the experiment , the positive drug and Kangfuxin solution are used in combination, the weight ratio of fluconazole to Kangfuxin solution is (1:1); the drug is diluted 5-fold, with 6 concentration gradients, and 3 replicate wells for each concentration gradient. Add 100 μL of different concentrations of liquid medicine and 100 μL of Candida albicans suspension to each we...

Embodiment 3

[0095] Experiment of Kangfuxin liquid combined with fluconazole against Candida albicans infection in vivo

[0096] Kangfuxin solution, bacterial strains and reagents used in the examples are all commercially available

[0097] 1. Grouping of animals

[0098] 150 SPF female Balb / c mice (4-6 weeks old, body weight 20±2g, animal qualification certificate number: SCXK (Dian) K2015-0002) were adaptively fed for 1 week and divided into 5 groups according to the random number table method. Groups, 30 rats in each group, were the control group, the fungal infection model group, the Kangfuxin liquid treatment group, the fluconazole treatment group, and the combined administration treatment group. The experimental period is 14 days. The mice in each group are fed with normal diet and raised in a clean animal room at 24±2°C with 12 hours of alternating light and dark. Before the experiment, each mouse is vaginally lavaged with sterile PBS and the lavage fluid is cultured. , select the...

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Abstract

The invention discloses an antifungal pharmaceutical composition consisting of a periplaneta americana extract and an antifungal drug. The pharmaceutical composition has the antifungal effect obviously superior to that of single use of periplaneta americana and fluconazole, and has better application prospects. The antifungal pharmaceutical composition provides a new thought for development and utilization of medicinal resources of the periplaneta americana, provides a new candidate drug and therapeutic scheme for clinical treatment of vulva vaginal candidiasis, and is expected to relieve painof vast female patients. The antifungal pharmaceutical composition has great significance in reasonably and sustainably developing and utilizing the natural drug resource of periplaneta americana.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an antifungal pharmaceutical composition composed of an extract of Periplaneta americana and an antifungal medicine. Background technique [0002] Traditional Chinese medicine believes that vulvovaginal candidiasis is mostly caused by damp heat and worms, and insects eat into the yin, and it belongs to the evil of exogenous dampness, turbidity and poison. The main cause of disease is deficiency of spleen-qi, stagnation of liver-qi, soaking of damp-qi, compulsion of heat-qi, and injury of the veins. In addition, due to inadvertent health care and unclean bathing utensils, it can be infected with damp toxins, directly invading the genitals, invading the two channels of the Ren channel, causing the Ren channel to become unstable, the channel to lose appointment, and dirty fluid to flow out. The drugs currently used clinically to treat vulvovaginal candidiasis, such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61K38/12A61P31/10A61P15/02A61K31/4196A61K31/496A61K31/506A61K31/7048A61K31/513A61K31/137A61K31/5375
CPCA61K31/137A61K31/4196A61K31/496A61K31/506A61K31/513A61K31/5375A61K31/7048A61K35/64A61K38/12A61P15/02A61P31/10A61K2300/00
Inventor 王睿睿杨亚超倪广惠张祎刘一丹苏刘艳李俊
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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