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A strain of Chaetomium monocele, bacterial agent including Chaetomium monocele, preparation method and application thereof

A technology of chaetomes and fungal agents, applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of single-row chaetomes and their application, so as to promote the improvement of soil fertility and operation Simple and effective in promoting the effective degradation of straw

Active Publication Date: 2021-03-02
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, currently there is very little research on Chaetomium monocele, and there is no existing technology to apply it to the agricultural field

Method used

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  • A strain of Chaetomium monocele, bacterial agent including Chaetomium monocele, preparation method and application thereof
  • A strain of Chaetomium monocele, bacterial agent including Chaetomium monocele, preparation method and application thereof
  • A strain of Chaetomium monocele, bacterial agent including Chaetomium monocele, preparation method and application thereof

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preparation example Construction

[0028] The present invention also provides a preparation method for the bacterial agent described in the above scheme, comprising the following steps:

[0029] 1) inoculating the Chaetomium monolecularis in PD liquid medium, and culturing at 25-30°C and 120-180rpm for 7-10 days to obtain the first fermentation mixture;

[0030] 2) inoculating the fermentation mixture on the fermentation medium, culturing at 25-30°C until the mycelium completely covers the fermentation medium, to obtain the first bacterial agent;

[0031] The fermentation medium comprises the following ingredients in parts by weight: 65-75 parts of corn stalks, 20-30 parts of bean cake powder, 2-4 parts of sucrose and 90-110 parts of water.

[0032] The present invention inoculates the chaetomium monofilament in PD liquid medium, and cultures it at 25-30°C and 120-180rpm for 7-10 days to obtain the first fermentation mixture; the chaetomium monofilament is preserved in the PDA medium The present invention has ...

Embodiment 1

[0043] Embodiment 1 single column Chaetomium F1 is to the plate confrontation experiment of Fusarium

[0044] Inoculate 1 / 4 and 3 / 4 of the diameter of a 90 mm PDA plate with activated Trichomonas and Fusarium, respectively, within 1 week. The Fusarium solani used was Fusarium solani, and the inoculation block diameter was 0.5 cm. Place it in a dark incubator at 25°C for 3 to 4 days, and observe the growth rate of the colony and whether there is an obvious antagonistic effect.

[0045] For test results, see figure 1 ,Depend on figure 1 It can be seen that although the growth rate of Fusarium is slightly faster than that of Trichoraceae, the extension of Fusarium hyphae around Trichoraceae is obviously restricted, and the colony forms a clear gap.

Embodiment 2

[0046] Embodiment 2 A kind of the first bacteria agent that comprises Chaetomium monocele of the present invention

[0047] 1. Inoculate vigorously growing mycelium blocks on PDA medium into PD liquid medium, place on a constant temperature shaker at 28°C and 220rpm for 10 days, and a large number of millet-shaped yellow mycelium balls will appear to obtain a fermentation mixture;

[0048] 2. Under sterile conditions, take 20mL of fermentation mixture and add it to 100g of fermentation medium (straw-soybean cake powder-sucrose medium), shake it up immediately; place it at 28°C for static cultivation; wait until the mycelium solidifies the straw bean cake sucrose medium After agglomeration, put the culture medium horizontally in the Erlenmeyer flask, so that both sides of the mushroom cake can be in contact with the air; then place it in a 28°C incubator until the mycelium completely covers the straw bean cake sucrose culture medium, and smash it with a glass rod, that is, Get ...

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Abstract

The invention provides a strain of Chaetomium monocele, its preparation method and application including Chaetomium monocele, and belongs to the technical field of microbial inoculum; the preservation number of the Chaetomium monocele is: CGMCC No.18156. The chaetomium monolezee of the invention promotes the improvement of soil fertility by promoting the effective degradation of straw, increasing the content of organic carbon and soluble carbon and nitrogen in the soil, and resisting pathogenic bacteria. The chaetomium monolectus of the present invention can grow and reproduce by itself in the soil, and reach an ecologically stable balance with the indigenous microbial community without repeated addition. The chaetomium monorow of the invention is derived from farmland soil where straws have been returned to the field for a long time, and can realize green ecological agriculture to ensure the high quality and safety of food. The enlarged culture process of the chaetomium monorow of the present invention is simple to operate and low in cost, and can be prepared into a microbial agent for popularization and application in the agricultural field.

Description

technical field [0001] The invention relates to the technical field of microbial inoculum, in particular to a strain of Chaetomium monocele, the inoculum comprising Chaetomium monocele and its preparation method and application. Background technique [0002] Chaetomium globosa was used as a biocontrol fungus earlier in the field of agriculture. Genome information shows that it has a complete enzyme system for degrading cellulose and hemicellulose, which has important research value in the development of lignocellulose-degrading fungi (Hao Xiaoran , Niu Xueliang, Li Qiang, et al., 2013. Differences in the ability of Chaetomium globosa to degrade natural lignocellulose and enzyme gene analysis, Biotechnology Communications). However, there is very little research on Chaetomyces monolezee at present, and there is no existing technology to apply it to the agricultural field. Contents of the invention [0003] The object of the present invention is to provide a strain of Chaet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/00C05F17/20C09K17/14C12R1/645C09K101/00
CPCC05F11/00C05F17/00C09K17/14C09K2101/00C12N1/14C12N1/145C12R2001/645C05F11/08Y02W30/40
Inventor 李芳韩燕来王祎姜瑛
Owner HENAN AGRICULTURAL UNIVERSITY
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