A strain of Chaetomium monocele, bacterial agent including Chaetomium monocele, preparation method and application thereof
A technology of chaetomes and fungal agents, applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of single-row chaetomes and their application, so as to promote the improvement of soil fertility and operation Simple and effective in promoting the effective degradation of straw
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[0028] The present invention also provides a preparation method for the bacterial agent described in the above scheme, comprising the following steps:
[0029] 1) inoculating the Chaetomium monolecularis in PD liquid medium, and culturing at 25-30°C and 120-180rpm for 7-10 days to obtain the first fermentation mixture;
[0030] 2) inoculating the fermentation mixture on the fermentation medium, culturing at 25-30°C until the mycelium completely covers the fermentation medium, to obtain the first bacterial agent;
[0031] The fermentation medium comprises the following ingredients in parts by weight: 65-75 parts of corn stalks, 20-30 parts of bean cake powder, 2-4 parts of sucrose and 90-110 parts of water.
[0032] The present invention inoculates the chaetomium monofilament in PD liquid medium, and cultures it at 25-30°C and 120-180rpm for 7-10 days to obtain the first fermentation mixture; the chaetomium monofilament is preserved in the PDA medium The present invention has ...
Embodiment 1
[0043] Embodiment 1 single column Chaetomium F1 is to the plate confrontation experiment of Fusarium
[0044] Inoculate 1 / 4 and 3 / 4 of the diameter of a 90 mm PDA plate with activated Trichomonas and Fusarium, respectively, within 1 week. The Fusarium solani used was Fusarium solani, and the inoculation block diameter was 0.5 cm. Place it in a dark incubator at 25°C for 3 to 4 days, and observe the growth rate of the colony and whether there is an obvious antagonistic effect.
[0045] For test results, see figure 1 ,Depend on figure 1 It can be seen that although the growth rate of Fusarium is slightly faster than that of Trichoraceae, the extension of Fusarium hyphae around Trichoraceae is obviously restricted, and the colony forms a clear gap.
Embodiment 2
[0046] Embodiment 2 A kind of the first bacteria agent that comprises Chaetomium monocele of the present invention
[0047] 1. Inoculate vigorously growing mycelium blocks on PDA medium into PD liquid medium, place on a constant temperature shaker at 28°C and 220rpm for 10 days, and a large number of millet-shaped yellow mycelium balls will appear to obtain a fermentation mixture;
[0048] 2. Under sterile conditions, take 20mL of fermentation mixture and add it to 100g of fermentation medium (straw-soybean cake powder-sucrose medium), shake it up immediately; place it at 28°C for static cultivation; wait until the mycelium solidifies the straw bean cake sucrose medium After agglomeration, put the culture medium horizontally in the Erlenmeyer flask, so that both sides of the mushroom cake can be in contact with the air; then place it in a 28°C incubator until the mycelium completely covers the straw bean cake sucrose culture medium, and smash it with a glass rod, that is, Get ...
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