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A method for fast sporulation of Cordyceps militaris

A technology of Cordyceps militaris and Cordyceps militaris, which is applied in botany equipment and methods, horticulture, agriculture, etc. It can solve the problems of long culture time on solid medium, difficulty in collecting ascospores, and inability to obtain them for a long time, so as to achieve short culture period and land occupation Small, non-polluting effect

Active Publication Date: 2022-04-29
ALPHA FUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Microcirculation spore production, using soil leaching solution as the basic medium, can quickly obtain conidia, but ascospores are not easy to collect and easy to pollute, and there are seasonal restrictions. They can only be collected during the Cordyceps harvest season, and cannot be obtained for a long time
[0004] Li Wenjia et al. provided a method for solid sporulation, in which the peat soil solid medium has a large sporulation capacity, which shows that the soil can promote the production of conidia, but the solid medium takes a long time to cultivate, and it takes 60-90 days, which is easy. Pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for fast sporulation of Mortierella sinensis, comprising the following steps:

[0040] S1 activation culture of Mortierella sinensis: inoculate Mortierella sinensis on PDA solid medium and cultivate for 45-60 days;

[0041] S2 medium preparation

[0042] S21 Extraction of soil leachate: Take 500g of soil and add 500ml of water, boil for 30min, filter, and sterilize at 121°C. The soil was taken from the common soil in the Cordyceps breeding area.

[0043] Preparation of S22 basic nutrient solution: Potassium dihydrogen phosphate 0.15g, magnesium sulfate 0.15g, calcium chloride 0.06g, water 1000ml, sterilized at 121°C for 30min;

[0044] S23 Mix the soil leach solution and the basic culture solution in a volume ratio of 1:10 to 1:20 to obtain a culture solution.

[0045] S3 inoculation: Cut the activated Mortierella sinensis into small bacterial blocks with a radius of 2 mm, and insert them into plates with culture medium. Each plate is connected with 15 ml of...

Embodiment 2

[0050] A method for fast sporulation of Mortierella sinensis, comprising the following steps:

[0051] S1 activation culture of Mortierella sinensis: inoculate Mortierella sinensis on PDA solid medium and culture for 45-60 days;

[0052] S2 medium preparation

[0053] S21 Extraction of soil leachate: Take 300-500g of soil and add 500ml of water, boil for 25-35min, filter, and sterilize at 121°C. The soil was taken from the common soil in the Cordyceps breeding area.

[0054] Preparation of S22 basic nutrient solution: After mixing 0.13-0.16g of potassium dihydrogen phosphate, 0.14-0.16g of magnesium sulfate, 0.04-0.07g of calcium chloride and 1000ml of water, sterilize at 121°C for 30min;

[0055] S23 Mix the soil leach solution and the basic culture solution in a volume ratio of 1:10 to 1:20 to obtain a culture solution.

[0056] S3 inoculation: Cut the activated Mortierella sinensis into 2mm pieces and insert them into plates with culture medium. Each plate is connected w...

Embodiment 3

[0072] A method for fast sporulation of Mortierella sinensis, comprising the following steps:

[0073] S1 activation culture of Mortierella sinensis: inoculate Mortierella sinensis on PDA solid medium and cultivate for 45 days;

[0074] S2 medium preparation

[0075] S21 Extraction of soil leachate: Take 500g of soil and add 500ml of water, boil for 25min, filter, and sterilize at 121°C. The soil was taken from the common soil in the Cordyceps breeding area.

[0076] Preparation of S22 basic nutrient solution: Mix 0.16g of potassium dihydrogen phosphate, 0.14g of magnesium sulfate, 0.07g of calcium chloride and 1000ml of water, and then sterilize at 121°C for 30min;

[0077] S23 Mix the soil leach solution and the basic culture solution at a volume ratio of 1:100 to obtain a culture solution.

[0078] S3 inoculation: cutting the activated Mortierella sinensis into 2.5mm bacterial blocks, and inserting them into plates with culture medium, each plate was connected with 16ml ...

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PUM

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Abstract

The present invention relates to a kind of Cordyceps militaris, be specifically related to a kind of method of fast sporulation of Cordyceps militaris, it comprises the following steps: Inoculate the strain of Cordyceps militaris into the culture solution containing soil leachate, cultivate in the dark until mycelium grows, filter Remove the mycelia to obtain conidia. The extraction of the soil leachate comprises the following steps: mixing the soil with distilled water, boiling until sterilization, and filtering to obtain the soil leachate. The method of the invention has short cultivation period, is not easy to be polluted, has wide sources of materials, is low in cost, and has no seasonal restrictions, and provides a stable source of conidia for infecting bat moth larvae.

Description

technical field [0001] The invention relates to a kind of Cordyceps militaris, in particular to a method for rapid sporulation of Cordyceps militaris. Background technique [0002] At present, the conidia of Cordyceps sinensis strains are obtained by PDA solid culture, solid fermentation culture and microcirculation spore production. [0003] Microcirculation spore production, using soil leachate as the basic medium, can quickly obtain conidia, but ascospores are not easy to collect and are easily polluted, and there are seasonal restrictions. They can only be collected during the Cordyceps harvest season, and cannot be obtained for a long time. [0004] Li Wenjia et al. provided a method for solid sporulation, in which the peat soil solid medium has a large sporulation capacity, which shows that the soil can promote the production of conidia, but the solid medium takes a long time to cultivate, and it takes 60-90 days, which is easy. Pollution. [0005] Mortierella sinens...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/40A01G18/20
Inventor 高益槐王忠吴鸿雪
Owner ALPHA FUJIAN BIOTECH
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