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Method for extracting and separating polysaccharide from codium fragile

A technology of pine algae and polysaccharides, applied in the direction of antiviral agents, etc., can solve the problems of difficult separation and low extraction rate of polysaccharides, and achieve the effect of large adsorption capacity, low viscosity of slurry, and easy filtration and separation

Inactive Publication Date: 2020-01-24
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems of low extraction rate and difficult separation of polysaccharides extracted from P. spinosa, the present invention provides a method for extracting and separating polysaccharides from P. spinosa. Water passes through fatty acid ether sodium sulfate and polysorbate-80 active polypeptide in advance to greatly promote cell wall The rupture and the release of polysaccharides reduce the extraction time and improve the extraction rate of polysaccharides, and the polysaccharides with anti-HPV activity are prepared by proper gradient elution in a strong anion exchange column

Method used

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  • Method for extracting and separating polysaccharide from codium fragile
  • Method for extracting and separating polysaccharide from codium fragile
  • Method for extracting and separating polysaccharide from codium fragile

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1) Dried P. chinensis at 40°C and crushed through a 60-mesh sieve for later use. Take pine algae powder, degrease by 85% ethanol reflux for 2 h, repeat twice, and then dry to obtain degreasing pine algae powder. Then carry out the treatment of destroying the cell wall: heat a small amount of water to 50 ° C, add 100 g of defatted pine algae powder and stir, add water in batches until the defatted pine algae powder just dissolves, mix 0.5 g of fatty acid ether sodium sulfate and 5 g of polysorbate- 80 was dissolved in ethanol whose volume was 5% of that of water, and the ethanol solution was added to the aqueous solution of degreased pine algae powder, stirred vigorously for 2 hours, centrifuged to obtain the water phase, filtered to obtain the filter cake, and dried for later use;

[0024] 2) Add about 20 times the volume of water to the dried pine algae, stir and extract with an electronic mixer in a water bath at 100 °C for 2 h, centrifuge, repeat the extraction of th...

Embodiment 2

[0031] 1) Take the degreased pine algae powder of Example 1, and carry out the treatment of destroying the cell wall: heat a small amount of water to 70°C, add 100g of the defatted pine algae powder and stir, add water in batches until the defatted pine algae powder just dissolves, and 2 1 g of fatty acid ether sodium sulfate and 3 g of polysorbate-80 were dissolved in ethanol whose volume was 20% of that of water, and the ethanol solution was added to the aqueous solution of degreased pine algae powder, stirred vigorously for 3 h, centrifuged to obtain the water phase, and filtered to obtain the filter cake , dry for later use;

[0032] 2) Add about 30 times the volume of water to the dried pine algae, stir and extract with an electronic mixer in a water bath at 80°C for 4 hours, centrifuge, repeat the extraction of the algae residue twice, combine the supernatants to concentrate and alcohol precipitation, and place in a refrigerator at 9°C Stand in the middle overnight, filt...

Embodiment 3

[0036] Take the degreased pine algae powder of Example 1, and carry out the treatment of destroying the cell wall: heat a small amount of water to 60 ° C, add 100 g of the defatted thorn pine algae powder and stir, add water in batches until the defatted thorn pine algae powder just dissolves, and 1 g Fatty acid ether sodium sulfate and 4 g polysorbate-80 were dissolved in ethanol whose volume was 10% of the volume of water, and the ethanol solution was added to the aqueous solution of degreased pine algae powder, stirred vigorously for 3 h, centrifuged to obtain the water phase, filtered to obtain the filter cake, Dry and reserve;

[0037] 2) Add about 20 times the volume of water to the dried pine algae, stir and extract with an electronic mixer in a water bath at 90 °C for 1 h, centrifuge, repeat the extraction of the algae residue twice, combine the supernatants to concentrate and alcohol precipitation, and store in a refrigerator at 3 °C Stand in the middle overnight, fil...

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Abstract

The invention relates to the technical field of polysaccharide extraction. Aiming at solving the problems of low polysaccharide extraction rate from codium fragile and difficult separation, the invention provides a method for extracting and separating polysaccharide from codium fragile. The method comprises the following steps: performing pretreatment comprising degreasing on codium fragile; thenperforming extracting on the codium fragile with hot water, performing alcohol precipitation, performing standing overnight, performing suction filtration, adding ethanol for washing, and carrying outdehydrating and drying to obtain a crude polysaccharide which is extracted from the codium fragile with hot water; and finally separating the crude polysaccharide which is extracted from the codium fragile with hot water by using a strong anion exchange chromatographic column: concretely performing gradient elution with an NaCl solution, collecting the polysaccharide by a fraction collector, performing pressure-reduced concentration on the eluent of the components, performing dialysis for desalination, and performing freeze drying to obtain the product polysaccharide. According to the method,cell wall rupture and polysaccharide release are greatly promoted through fatty acid ether sodium sulfate and polysorbate-80 active polypeptide in advance, so that the extraction time is reduced, thepolysaccharide extraction rate is reduced, and the polysaccharide with anti-HPV activity is prepared through proper gradient elution in the strong anion exchange chromatographic column.

Description

technical field [0001] The invention relates to the technical field of extracting polysaccharides, in particular to a method for extracting and separating polysaccharides from P. chinensis. Background technique [0002] Echinococcus is a perennial green algae belonging to the Chlorophyta. The algae are dendritic, mostly staghorn-shaped branches, cylindrical branches, and a few are flat. The interior is a sponge-shaped structure composed of intertwined tubular filaments, and the surface has closely arranged filaments to form a club-shaped capsule. The extraction of macromolecules containing sugars from P. chinensis has been studied for a long time. For example, love J et al. proved that the water extract of the algae contains glucan, mannan, highly sulfated and pyruvated galactan and Sulfated arabinan. However, the separation and purification of the extracted polysaccharides is still a difficult problem. In addition, the cell wall of this seaweed is a highly assembled stru...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P31/20
CPCA61P31/20C08B37/0003C08B37/006
Inventor 陈荫王腾董喆孙坤来赵玉勤王斌
Owner ZHEJIANG OCEAN UNIV
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