Ph and reducing agent-responsive carrier and preparation method based on pseudorotaxane molecular structure

A technology of molecular structure and quasi-rotaxane, applied in the field of material science, can solve the problems of high release baseline, the inability of nanocarriers to achieve multiple responses, and the long time required to achieve the effect of improving species

Active Publication Date: 2020-10-16
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention solves the technical problems that nanocarriers in the prior art cannot realize multiple responses, have a high release baseline under neutral conditions, are explosive during the preparation process, and require a long time

Method used

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  • Ph and reducing agent-responsive carrier and preparation method based on pseudorotaxane molecular structure
  • Ph and reducing agent-responsive carrier and preparation method based on pseudorotaxane molecular structure
  • Ph and reducing agent-responsive carrier and preparation method based on pseudorotaxane molecular structure

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Experimental program
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Effect test

Embodiment 1

[0041] In the present invention, a multiple-response (pH, reducing agent) drug-carrying container based on the pseudorotaxane supramolecular structure comprises the following steps:

[0042] (1) Disperse 200mg of hollow silicon oxide material in xylene and heat to 100°C, then add 72.72mg of coupling agent KH590 (mercaptopropyltrimethoxysilane), and carry out surface modification reaction for 12h;

[0043] (2) 150 mg of the product obtained in step (1) was dispersed in 15% methanol aqueous solution with pH = 4.5, 75 mg of 2,2-dithiobipyridine was added, and disulfide bond exchange reaction was carried out at room temperature, and the reaction time was 4 hours;

[0044] (3) Take 100 mg of the product obtained in step (2) and disperse it in 15% methanol aqueous solution with pH = 4.5, add 100 mg of cysteine ​​to react, and carry out disulfide bond exchange reaction at room temperature for 0.25 h;

[0045] (4) The product obtained in step (3) was dispersed in 6 g of high-concentra...

Embodiment 2

[0050] In the present invention, a multiple-response (pH, reducing agent) drug-carrying container based on the pseudorotaxane supramolecular structure comprises the following steps:

[0051] (1) Disperse 200mg of hollow zirconia material in xylene and heat to 110°C, then add 30.3mg of mercaptopropyltributoxysilane coupling agent, and carry out surface modification reaction for 16h;

[0052] (2) 150 mg of the product obtained in step (1) was dispersed in 15% methanol aqueous solution with pH = 3.5, 75 mg of 2,2-dithiobipyridine was added, and disulfide bond exchange reaction was carried out at room temperature, and the reaction time was 2 hours;

[0053] (3) Take 100 mg of the product obtained in step (2) and disperse it in 15% methanol aqueous solution with pH = 3.5, add 100 mg of cysteine ​​to react, and carry out disulfide bond exchange reaction at room temperature for 30 min;

[0054] (4) The product obtained in step (3) was dispersed in 6 g of high-concentration rhodamine ...

Embodiment 3

[0058] In the present invention, a multiple-response (pH, reducing agent) drug-carrying container based on the pseudorotaxane supramolecular structure comprises the following steps:

[0059] (1) Disperse 200mg of hollow silica material in toluene and heat to 80°C, then add 150mg of mercaptopropyldimethoxysilane, and carry out surface modification reaction for 24 hours;

[0060] (2) 150 mg of the product obtained in step (1) was dispersed in 15% methanol aqueous solution with pH=5.5, 75 mg of 2,2-dithiobipyridine was added, and disulfide bond exchange reaction was carried out at room temperature, and the reaction time was 6 hours;

[0061] (3) Take 100 mg of the product obtained in step (2) and disperse it in 15% methanol aqueous solution with pH = 5.5, add 100 mg of cysteine ​​to react, and carry out disulfide bond exchange reaction at room temperature for 2 h;

[0062] (4) The product obtained in step (3) is dispersed in 6 g of high-concentration rhodamine drug aqueous soluti...

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Abstract

The invention relates to a pH and reducing agent responsive carrier based on a pseudorotaxane molecular structure and a preparation method, and belongs to the field of material science. The carrier comprises a hollow mesoporous inorganic oxide and pseudorotaxane molecules, wherein the pseudorotaxane molecules comprise chain molecules and cyclic molecules having a lyophobic cavity, one ends of thechain molecules are silane couplers, the other ends of the chain molecules are cysteine residues, and the silane couplers and the cysteine residues are connected through disulfide bonds; the silane couplers are connected with the hollow mesoporous inorganic oxide through a covalent bond, and the cyclic molecules having the lyophobic cavity form a molecule protective screen of the hollow mesoporousinorganic oxide; and the cysteine residues can enable the cyclic molecules having the lyophobic cavity to come off under the condition of acidity or alkalinity, and the disulfide bonds can crack under the action of a reducing agent. The carrier disclosed by the invention can realize medicine release in acid and alkaline environment and reducing environment.

Description

technical field [0001] The invention belongs to the field of material science, and in particular relates to a pH- and reducing agent-responsive carrier based on the molecular structure of a pseudorotaxane and a preparation method thereof. Background technique [0002] Nano-drug delivery systems have gradually become the most promising platform for chemotherapy drug delivery, thanks to their targeted drug delivery in cancer treatment, which not only greatly reduces the damage caused by chemotherapy to normal tissues, but also enhances the effect of chemotherapy drugs on cancer cells. organizational lethality. An ideal nano-drug delivery system for cancer treatment must not only have the most basic ability to respond in cancer cells, but also need to be able to enrich near cancer cells, deeply penetrate cancer cells, and be effectively taken up by cancer cells. In addition to the application in anti-cancer treatment, the environment-responsive drug-loading system can also be ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K31/5377A61P35/00
CPCA61K31/5377A61K47/6923A61K47/6951A61P35/00
Inventor 付朝阳杨珊珊陈展陈天奇
Owner HUAZHONG UNIV OF SCI & TECH
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