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Intermediate rhizobium strain with phosphorus-dissolving property and application thereof

An intermediate rhizobia and strain technology, applied in the application, bacteria, organic fertilizer and other directions, can solve the problem of rare research and development reports on phosphorus-solubilizing bacteria, and achieve the effects of improving growth conditions, high-efficiency phosphorus-solubilizing properties, and increasing effective phosphorus content

Active Publication Date: 2020-01-21
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, my country has developed phosphate-solubilizing bacteria fertilizers suitable for certain soils and crops, and has been applied in the production of melons and fruits, vegetables, peanuts, Chinese medicinal materials, wheat and other crops in some areas, but it is suitable for acidic red soil and promoting corn. There are relatively few reports on the research and development of phosphate-solubilizing bacteria that increase production

Method used

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  • Intermediate rhizobium strain with phosphorus-dissolving property and application thereof
  • Intermediate rhizobium strain with phosphorus-dissolving property and application thereof
  • Intermediate rhizobium strain with phosphorus-dissolving property and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Screening and Identification of Mesorhizobium.sp Strain YTR-M3

[0025] 1. Screening process

[0026] (1) Weigh 1g of rhizosphere soil from the high-fertilizer treatment of Jiangxi Yingtan Red Soil Experiment Station's long-term positioning test site and put it into a centrifuge tube, add 9mL of 0.9% normal saline to make 10 -1 The bacterial suspension was placed in a shaking box at 180r / min for 10 minutes, and then left to stand for 1 hour after taking it out.

[0027] (2) Draw 5 μL of the static soil suspension under sterile conditions and add 45 μL sterile water to dilute to 10 -2 bacterial suspension. The bacterial suspension was diluted to 10 -3 、10 -4 、10 -5 , respectively take 5mL 10 -5 The bacterial suspension was added to 50mL and 500mL of LB medium to make 10 -6 and 10 -7 culture medium, the 10 -6 and 10 -7 Add 250 μL of culture solution to a 96-well plate, and add three plates for each concentration. Finally, the 96-well plate was placed in a micro...

Embodiment 2

[0031] Phosphorus Solubilizing Properties of Mesorhizobium.sp Strain YTR-M3

[0032] (1) The strain YTR-M3 was stored in a glycerol tube, and after activation, it was streaked on LB solid medium and stored for later use.

[0033] (2) Prepare Montina organophosphate (lecithin), aluminum phosphate, and iron phosphate medium plates. Each plate is divided into 6 areas, and 6 μL points are drawn in the center of each area in turn. Cultivate for 4 days, observe and record the diameter of the phosphorus-dissolving circle (unit D / cm), the diameter of the colony (unit d / cm), and calculate the D / d ratio to determine the phosphorus-dissolving ability and efficiency of the strain for different insoluble phosphorus. The formula of the culture medium is as follows: ①Mengkina organic phosphorus (lecithin) bacterial culture medium (1L) glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 0.03g, MnSO 4 ·H 2 O 0.03g, KCl 0.3g, CaCO 3 1.0g, lecithin 0.3g, agar 2...

Embodiment 3

[0038] Corn pot experiment

[0039] 1. A total of 3 treatments were set up in the experiment, which were control, wood vinegar (wood vinegar compound water-soluble fertilizer containing amino acids), wood vinegar + bacterial agent (YTR-M3), and 4 replicates for each treatment.

[0040] 2. Dosage of soil and fertilizer: about 500g of soil in each 10×10(cm) pot, add 15mL of wood vinegar (1:800 dilution) to each pot as base fertilizer. Then apply every other week as a root drip and foliar spray.

[0041] 3. Seedling cultivation: 1) Disinfect seeds, select appropriate amount of seeds, first disinfect with 70% alcohol for 1 min, then disinfect with 1% sodium hypochlorite for 15 min, and finally rinse with sterile water five times. Put the sterilized seeds on sterile water, place them in a light incubator at 28° C. and wait for them to germinate; 2) select seedlings with consistent growth and transplant them into red soil on the fifth day after the seeds germinate.

[0042] 4. Add...

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Abstract

The invention discloses an intermediate rhizobium strain with phosphorus-dissolving property and application thereof. The intermediate rhizobium strain is a Mesorhizobium. sp YTR-M3 strain which is preserved in the China general microbiological culture collection center with the preservation number CGMCC No.17532 on April 9, 2019. A strain of high-efficiency phosphate-dissolving bacteria is screened from soil of a long-term organic fertilization location test plot of Yingtan Red Soil Ecological Experiment Station of the Chinese Academy of Sciences, the strain of the high-efficiency phosphate-dissolving bacteria is identified, the fact that the strain YTR-M3 has high-efficient phosphorus-dissolving property is proved through experiments, by being mixed use with wood vinegar liquid, the effective phosphorus content in red soil is significantly increased, and the growth condition of corn is improved.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to an intermediate rhizobia strain YTM-3 and its application in phosphorus solubilization. Background technique [0002] Phosphorus is the second largest inorganic nutrient element necessary for plants, and it participates in many important enzymatic reactions and metabolic regulation in plants. Therefore, phosphorus is of great significance to the growth and metabolism of plants. The red soil in the southeastern hilly area is mainly distributed in subtropical and tropical regions, including Hunan, Jiangxi, Zhejiang, Fujian, Guangxi, Guangdong, Qiong and southern Hubei, Jiangsu, and Anhui, with an area of ​​about 1.13 million km 2 , accounting for 11.8% of the total land area of ​​the country. Phosphorus exists in various chemical forms in red soil, among which inorganic phosphorus accounts for 35%-70% of total phosphorus, and organic phosphorus acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/20A01P21/00C05G3/80C12R1/01C09K101/00
CPCA01N63/00C05F11/00C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 蒋瑀霁孙波郑洁张伟陈利军
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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