Extraction method of nostoc sphaeroids kutz polysaccharide

An extraction method and technology of kudzu, applied in the chemical field, can solve the problems of low extraction rate of kudzu polysaccharide, poor product solubility, turbidity, etc., and achieve the effects of good moisturizing effect, antioxidant capacity, and scientific and reasonable design.

Inactive Publication Date: 2020-01-21
上海植森生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the extraction rate of polysaccharides of Gexianmi in this process is low, the solubility of the product is poor, and the solution is easy to precipitate again and become turbid after a short period of storage.

Method used

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  • Extraction method of nostoc sphaeroids kutz polysaccharide

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Experimental program
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Effect test

Embodiment 1

[0029] A method for extracting polysaccharides from Pueraria lobata, comprising the following steps:

[0030] S1. Pre-crushing: pulverize the dried Gexian rice product with a pulverizer to obtain Gexian rice powder;

[0031] S2. Enzymatic hydrolysis: Weigh 50g of Pueraria lobata powder in the extraction tank, add 2500ml parts by weight of pure water to dissolve, pour into 0.25% alkaline protease after dissolution, and adjust the pH of the material solution to 8 with sodium hydroxide. Put it in a 52℃ water bath and stir for 1.5h; after enzymolysis, adjust the pH of the material solution to 6.0, increase the temperature to 85℃ and keep it for 0.5h; then add a high-temperature amylase solution with a volume fraction of 0.5%, and hydrolyze at 85℃ for 1h , To obtain the Gexianmi enzymatic hydrolysis mixture;

[0032] S3. Acidolysis and decolorization: Add 0.6% 6% dilute hydrochloric acid to the mixture of Gexianmi enzymatic hydrolysis, and stir evenly; then add 1000ppm ClO 2 The solution...

Embodiment 2

[0039] A method for extracting polysaccharides from Pueraria lobata, comprising the following steps:

[0040] S1. Pre-crushing: pulverize the dried Gexian rice product with a pulverizer to obtain Gexian rice powder;

[0041] S2. Enzymatic hydrolysis: Weigh 50g of Pueraria lobata powder in an extraction tank, add 2000ml parts by weight of pure water to dissolve, pour into 0.25% alkaline protease after dissolution, and adjust the pH of the material solution to 7.5 with sodium hydroxide. Put it in a 50℃ water bath and stir for 1 hour; after the enzymolysis, adjust the pH of the material solution to 6.0, raise the temperature to 85℃ and keep it for 0.4h; then add a high-temperature amylase solution with a volume fraction of 0.5%, and hydrolyze at 85℃ for 0.8h , To obtain the Gexianmi enzymatic hydrolysis mixture;

[0042] S3. Acidolysis and decolorization: Add 0.6% 6% dilute hydrochloric acid to the mixture of Gexianmi enzymatic hydrolysis, and stir evenly; then add 1000ppm ClO 2 The so...

Embodiment 3

[0049] A method for extracting polysaccharides from Pueraria lobata, comprising the following steps:

[0050] S1. Pre-crushing: pulverize the dried Gexian rice product with a pulverizer to obtain Gexian rice powder;

[0051] S2. Enzymatic hydrolysis: Weigh 50g of Pueraria lobata powder in the extraction tank, add 2500ml parts by weight of pure water to dissolve, pour into 0.25% alkaline protease after dissolution, adjust the pH of the material liquid to 8.5 with sodium hydroxide, Put it in a 55℃ water bath and stir for 2h; after the enzymolysis, adjust the pH of the material solution to 6.0, heat up to 85℃ and keep it for 0.6h; then add 0.5% high-temperature amylase solution and hydrolyze at 85℃ for 1.2h , To obtain the Gexianmi enzymatic hydrolysis mixture;

[0052] S3. Acidolysis and decolorization: Add 0.6% 6% dilute hydrochloric acid to the mixture of Gexianmi enzymatic hydrolysis, and stir evenly; then add 1000ppm ClO 2 The solution was stirred at 80°C for 2h to obtain a primar...

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Abstract

The invention belongs to the technical field of chemical engineering, and particularly relates to an extraction method of nostoc sphaeroids kutz polysaccharide. According to the extraction method of the nostoc sphaeroids kutz polysaccharide, a dry nostoc sphaeroids kutz product is subjected to pre-crushing, enzymolysis, acidolysis decoloration, centrifugal filtration, primary alcohol precipitation, redissolution, secondary alcohol precipitation and drying to obtain a nostoc sphaeroids kutz polysaccharide substance, which has certain viscosity and film-forming property, has good moisturizing and antioxidant capacity and can be applied to various cosmetics. The extraction method provided by the invention is scientific and reasonable in design, and solves the problems that the nostoc sphaeroides polysaccharide extraction rate is low, the product solubility is poor, the solution is easy to precipitate again after being stored for a short time and becomes turbid and the like in the traditional process.

Description

Technical field [0001] The invention belongs to the technical field of chemical engineering, and in particular relates to a method for extracting polysaccharides from Pueraria lobata. Background technique [0002] The scientific name of Ge Xian Mi is Nostoc globularis, and it is a member of the cyanobacteria. Gexianmi is mainly distributed in Hefeng County, Hubei Province, and is more common in paddy fields. The body of Gexianmi algae is spherical, composed of many single cells, and the outermost layer is a transparent colloid. Polysaccharide is the main component of Gexian rice, which accounts for 10% to 38% of the dry matter weight. Ge Xian Mi has a long history of edible and medicinal use in my country. Studies have found that Ge Xian Mi polysaccharide has anti-inflammatory, antioxidant, antibacterial, anti-tumor cell proliferation and immune regulation functions. At present, the health function of Gexianmi polysaccharide is receiving more and more attention and research. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 骆峰杨升平赖志华董婷婷
Owner 上海植森生物工程有限公司
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